Literature summary for 5.2.1.1 extracted from
Liu, X.; Zhao, Q.; Ren, J.; Dong, W.; Wu, Q.; Zhu, D.
N-terminal truncation of a maleate cis-trans isomerase from Rhodococcus jostii RHA1 results in a highly active enzyme for the biocatalytic production of fumaric acid (2013), J. Mol. Catal. B, 93, 44-50.
No PubMed abstract available
Application
Application |
Comment |
Organism |
synthesis |
whole cells of Escherichia coli expressing the enzyme mutant MaiR-D48 catalyze the isomerization of maleic acid to fumaric acid at 1 M substrate concentration showing its potential for industrial use |
Rhodococcus jostii |
Cloned(Commentary)
Cloned (Comment) |
Organism |
gene ro00478, recombinant expression of wild-type and mutants in Escherichia coli Rosetta2 (DE3) pLysS |
Rhodococcus jostii |
Protein Variants
Protein Variants |
Comment |
Organism |
additional information |
generation of three catalytically active mutants MaiR-D41/42-304AA, MaiR-D48/49-304AA and MaiR-D52/53-304AA without the hydrophilic and hydrophobic clusters |
Rhodococcus jostii |
KM Value [mM]
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Comment |
Organism |
Structure |
0.1496 |
- |
Maleate |
pH 8.0, 40°C, recombinant enzyme mutant MaiR-D48/49-304AA |
Rhodococcus jostii |
|
Natural Substrates/ Products (Substrates)
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
Maleate |
Rhodococcus jostii |
whole cells of recombinant Escherichia coli expressing enzyme mutant MaiR-D48 catalyze the isomerization of maleic acid to fumaric acid at 1 M substrate concentration |
Fumarate |
- |
r |
|
Organism
Organism |
UniProt |
Comment |
Textmining |
Rhodococcus jostii |
Q0SJH2 |
gene ro00478 |
- |
Oxidation Stability
Oxidation Stability |
Organism |
enzyme mutant MaiR-D48 shows high oxidative stability |
Rhodococcus jostii |
Purification (Commentary)
Purification (Comment) |
Organism |
recombinant mutant MaiR-D48/49-304AA 2.7fold from Escherichia coli strain Rosetta 2 (DE3) by ammonium sulfate fractionation, anion exchange chromatography, ultratfiltration, and gel filtration |
Rhodococcus jostii |
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Comment |
Organism |
106.8 |
- |
purified enzyme mutant MaiR-D48/49-304AA, pH 8.0, 40°C |
Rhodococcus jostii |
Substrates and Products (Substrate)
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
Maleate |
- |
Rhodococcus jostii |
Fumarate |
- |
r |
|
Maleate |
whole cells of recombinant Escherichia coli expressing enzyme mutant MaiR-D48 catalyze the isomerization of maleic acid to fumaric acid at 1 M substrate concentration |
Rhodococcus jostii |
Fumarate |
- |
r |
|
Synonyms
Synonyms |
Comment |
Organism |
maleate cis-trans isomerase |
- |
Rhodococcus jostii |
Temperature Optimum [°C]
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
40 |
- |
recombinant mutant MaiR-D48/49-304AA |
Rhodococcus jostii |
Temperature Range [°C]
Temperature Minimum [°C] |
Temperature Maximum [°C] |
Comment |
Organism |
25 |
60 |
activity range, recombinant mutant MaiR-D48/49-304AA |
Rhodococcus jostii |
Temperature Stability [°C]
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Comment |
Organism |
40 |
- |
purified recombinant mutant MaiR-D48/49-304AA, 30 min, completely stable |
Rhodococcus jostii |
55 |
60 |
purified recombinant mutant MaiR-D48/49-304AA, 30 min, over 95% activity at 55°C and about 50% activity at 60°C remaining |
Rhodococcus jostii |
Turnover Number [1/s]
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Comment |
Organism |
Structure |
96.7 |
- |
Maleate |
pH 8.0, 40°C, recombinant enzyme mutant MaiR-D48/49-304AA |
Rhodococcus jostii |
|
pH Optimum
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
8 |
- |
recombinant mutant MaiR-D48/49-304AA |
Rhodococcus jostii |
pH Range
pH Minimum |
pH Maximum |
Comment |
Organism |
6 |
9 |
activity range, recombinant mutant MaiR-D48/49-304AA |
Rhodococcus jostii |
kcat/KM [mM/s]
kcat/KM Value [1/mMs-1] |
kcat/KM Value Maximum [1/mMs-1] |
Substrate |
Comment |
Organism |
Structure |
650 |
- |
Maleate |
pH 8.0, 40°C, recombinant enzyme mutant MaiR-D48/49-304AA |
Rhodococcus jostii |
|