Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | - |
Alcaligenes faecalis | |
dithiothreitol | activates | Alcaligenes faecalis |
Cloned (Comment) | Organism |
---|---|
- |
Alcaligenes faecalis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
H2O2 | - |
Alcaligenes faecalis | |
iodoacetamide | - |
Alcaligenes faecalis | |
N-Chlorosuccinimide | - |
Alcaligenes faecalis | |
p-chloromercuriphenylsulfonate | - |
Alcaligenes faecalis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
Maleate | - |
Alcaligenes faecalis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27971 | - |
2 * 27971, calculation from nucleotide sequence | Alcaligenes faecalis |
28000 | - |
2 * 28000, SDS-PAGE | Alcaligenes faecalis |
60000 | - |
gel filtration | Alcaligenes faecalis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Alcaligenes faecalis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Alcaligenes faecalis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Maleate | r, reverse reaction is quite weak | Alcaligenes faecalis | Fumarate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 28000, SDS-PAGE | Alcaligenes faecalis |
dimer | 2 * 27971, calculation from nucleotide sequence | Alcaligenes faecalis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
42 | 45 | - |
Alcaligenes faecalis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | 50 | about 50% of maximal activity at 35°C and 50°C | Alcaligenes faecalis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
36.7 | - |
Maleate | - |
Alcaligenes faecalis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Alcaligenes faecalis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 9.3 | about 50% of maximal activity at pH 6.5 and at pH 9.3 | Alcaligenes faecalis |
7 | 9 | more than 75% of maximal activity at pH 7.0 and pH 9.0 | Alcaligenes faecalis |