Literature summary for 5.2.1.1 extracted from

Scher, W.; Jacoby, W.B.
Maleate isomerase (1969), J. Biol. Chem., 244, 1878-1882.

Search for more literature for 5.2.1.1

Activating Compound

Activating Compound	Comment	Organism	Structure
mercaptans	required	Pseudomonas fluorescens

Inhibitors

Inhibitors	Comment	Organism	Structure
Bromoacetate	-	Pseudomonas fluorescens

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
74000	-	sedimentation velocity experiments	Pseudomonas fluorescens

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas fluorescens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Pseudomonas fluorescens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
24.4	-	-	Pseudomonas fluorescens

Storage Stability

Storage Stability	Organism
-20°C, overnight, 10% loss of activity	Pseudomonas fluorescens
4°C, 20% glycerol, 10 mM thioglycerol, 3 months, 70% loss of activity	Pseudomonas fluorescens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
Maleate	reverse reaction is not observed	Pseudomonas fluorescens	Fumarate	-	?

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
30	-	Maleate	-	Pseudomonas fluorescens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8.4	-	-	Pseudomonas fluorescens

pH Range

pH Minimum	pH Maximum	Comment	Organism
6.7	9.4	-	Pseudomonas fluorescens

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Release 2023.1 (January 2023)