Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | - |
Alcaligenes faecalis | |
cyanide | KCN, 1 mM, 20% inhibition | Alcaligenes faecalis | |
EDTA | 1 mM, 7% inhibition | Alcaligenes faecalis | |
Hg2+ | - |
Alcaligenes faecalis | |
iodoacetate | 1 mM 73% inhibition | Alcaligenes faecalis | |
PCMB | 0.1 mM, complete inhibition; inhibition is overcome by the addition of sulfhydryl compounds | Alcaligenes faecalis | |
Sodium periodate | 1 mM, complete inhibition | Alcaligenes faecalis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.8 | - |
Maleate | - |
Alcaligenes faecalis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Alcaligenes faecalis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Alcaligenes faecalis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Alcaligenes faecalis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Maleate | reverse reaction is not observed | Alcaligenes faecalis | Fumarate | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
15 min, stable below | Alcaligenes faecalis |
40 | - |
15 min, about 25% loss of activity | Alcaligenes faecalis |
50 | - |
15 min, complete loss of activity | Alcaligenes faecalis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.3 | - |
- |
Alcaligenes faecalis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 9.3 | pH 7: about 60% of maximal activity, pH 9.3: about 40% of maximal activity | Alcaligenes faecalis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 7 | stable over this range in presence of dithiothreitol for a few weeks. In absence of dithiothreitol the activity is markedly decreased, especially in the alkaline region | Alcaligenes faecalis |
6 | - |
most stable in acetate buffer at this pH | Alcaligenes faecalis |