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Literature summary for 5.1.99.6 extracted from

  • Niehaus, T.D.; Richardson, L.G.; Gidda, S.K.; Elbadawi-Sidhu, M.; Meissen, J.K.; Mullen, R.T.; Fiehn, O.; Hanson, A.D.
    Plants utilize a highly conserved system for repair of NADH and NADPH hydrates (2014), Plant Physiol., 165, 52-61.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.1.99.6

Cloned(Commentary)

Cloned (Comment) Organism
expression of N-terminally His-tagged enzyme in Escherichia coli Zea mays
expression of N-terminally His-tagged enzyme in Escherichia coli Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0006
-
 (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide pH and temperature not specified in the publication Arabidopsis thaliana
0.00115
-
 (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate pH and temperature not specified in the publication Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
 Zea mays 9507
-
chloroplast
-
 Arabidopsis thaliana 9507
-
cytoplasm
-
 Zea mays 5737
-
cytoplasm
-
 Arabidopsis thaliana 5737
-
mitochondrion
-
 Zea mays 5739
-
mitochondrion
-
 Arabidopsis thaliana 5739
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide Zea mays i.e. NADHX (6R)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide
-
 r
(6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide Arabidopsis thaliana i.e. NADHX (6R)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide
-
 r
(6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate Zea mays i.e. NADPHX (6R)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate
-
 r
(6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate Arabidopsis thaliana i.e. NADPHX (6R)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate
-
 r

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q9LTX3
-
-
Zea mays
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant N-terminally His-tagged enzyme from Escherichia coli by nickel affinity chromatography Zea mays
recombinant N-terminally His-tagged enzyme from Escherichia coli by nickel affinity chromatography Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining
seedling
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide i.e. NADHX Zea mays (6R)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide
-
 r
(6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide i.e. NADHX Arabidopsis thaliana (6R)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide
-
 r
(6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate i.e. NADPHX Zea mays (6R)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate
-
 r
(6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate i.e. NADPHX Arabidopsis thaliana (6R)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate
-
 r

Synonyms

Synonyms Comment Organism
epimerase-PPOX
-
 Zea mays
epimerase-PPOX
-
 Arabidopsis thaliana

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.26
-
 (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate pH and temperature not specified in the publication Arabidopsis thaliana
0.38
-
 (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide pH and temperature not specified in the publication Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
additional information the hydratase is dependent on ATP, but the epimerase ist not Zea mays
additional information the hydratase is dependent on ATP, but the epimerase ist not Arabidopsis thaliana

General Information

General Information Comment Organism
malfunction a truncated form of the Arabidopsis epimerase lacking the PPOX domain is also fully active in the interconversion Arabidopsis thaliana
additional information the epimerase homologue is fused to the vitamin B6 salvage enzyme pyridoxine 5'-phosphate oxidase, PPOX. Plant epimerase homologs have an extra C-terminal domain that belongs to the pyridoxine/pyridoxamine phosphate oxidase (PPOX) family Zea mays
additional information the epimerase homologue is fused to the vitamin B6 salvage enzyme pyridoxine 5'-phosphate oxidase, PPOX. Plant epimerase homologs have an extra C-terminal domain that belongs to the pyridoxine/pyridoxamine phosphate oxidase (PPOX) family Arabidopsis thaliana
physiological function NADH and NADPH undergo spontaneous and enzymatic reactions that produce R and S forms of NAD(P)H hydrates, which are not electron donors and inhibit various dehydrogenases. The highly conserved epimerase repairs the hydrates formed from NADH and NADPH in plants together with a highly conserved dehydratase targeted to multiple compartments Zea mays
physiological function NADH and NADPH undergo spontaneous and enzymatic reactions that produce R and S forms of NAD(P)H hydrates, which are not electron donors and inhibit various dehydrogenases. The highly conserved epimerase repairs the hydrates formed from NADH and NADPH in plants together with a highly conserved dehydratase targeted to multiple compartments Arabidopsis thaliana