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Literature summary for 5.1.99.5 extracted from

  • Las Heras-Vazquez, F.J.; Martinez-Rodriguez, S.; Mingorance-Cazorla, L.; Clemente-Jimenez, J.M.; Rodriguez-Vico, F.
    Overexpression and characterization of hydantoin racemase from Agrobacterium tumefaciens C58 (2003), Biochem. Biophys. Res. Commun., 303, 541-547.
    View publication on PubMed
Search for more literature for 5.1.99.5

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Agrobacterium tumefaciens

General Stability

General Stability Organism
enzyme is active after 10 freeze-thawing cycles Agrobacterium tumefaciens

Inhibitors

Inhibitors Comment Organism Structure
Co2+ slight inhibition Agrobacterium tumefaciens
Cu2+ strong inhibition Agrobacterium tumefaciens
Hg2+ strong inhibition Agrobacterium tumefaciens
additional information not inhibitory: EDTA, Mn2+, dithiothreitol Agrobacterium tumefaciens
Ni2+ slight inhibition Agrobacterium tumefaciens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.23
-
 L-isobutylhydantoin pH 7.5, 40°C Agrobacterium tumefaciens
4.26
-
 D-ethylhydantoin pH 7.5, 40°C Agrobacterium tumefaciens
4.45
-
 L-ethylhydantoin pH 7.5, 40°C Agrobacterium tumefaciens
4.47
-
 D-5-methylthioethylhydantoin pH 7.5, 40°C Agrobacterium tumefaciens
4.58
-
 D-isobutylhydantoin pH 7.5, 40°C Agrobacterium tumefaciens
4.71
-
 D-benzylhydantoin pH 7.5, 40°C Agrobacterium tumefaciens
5.41
-
 L-5-methylthioethylhydantoin pH 7.5, 40°C Agrobacterium tumefaciens
5.56
-
 L-benzylhydantoin pH 7.5, 40°C Agrobacterium tumefaciens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32000
-
 4 * 32000, SDS-PAGE Agrobacterium tumefaciens
100000
-
 gel filtration Agrobacterium tumefaciens

Organism

Organism UniProt Comment Textmining
Agrobacterium tumefaciens
-
-
-
Agrobacterium tumefaciens C58 / ATCC 33970
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein Agrobacterium tumefaciens

Storage Stability

Storage Stability Organism
-20°C, pH 8.0, 20% glycerol, stable for at least 6 months Agrobacterium tumefaciens
4°C, pH 8.0, stable for 8 weeks Agrobacterium tumefaciens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-5-methylthioethylhydantoin
-
 Agrobacterium tumefaciens D-5-methylthioethylhydantoin
-
 r
L-5-methylthioethylhydantoin
-
 Agrobacterium tumefaciens C58 / ATCC 33970 D-5-methylthioethylhydantoin
-
 r
L-benzylhydantoin slow racemization Agrobacterium tumefaciens D-benzylhydantoin
-
 r
L-benzylhydantoin slow racemization Agrobacterium tumefaciens C58 / ATCC 33970 D-benzylhydantoin
-
 r
L-ethylhydantoin
-
 Agrobacterium tumefaciens D-ethylhydantoin
-
 r
L-ethylhydantoin
-
 Agrobacterium tumefaciens C58 / ATCC 33970 D-ethylhydantoin
-
 r
L-isobutylhydantoin
-
 Agrobacterium tumefaciens D-isobutylhydantoin
-
 r
L-isobutylhydantoin
-
 Agrobacterium tumefaciens C58 / ATCC 33970 D-isobutylhydantoin
-
 r
additional information preference for hydantoins with short rather than long aliphatic side chains or hydantoins with aromatic rings Agrobacterium tumefaciens ?
-
 ?
additional information preference for hydantoins with short rather than long aliphatic side chains or hydantoins with aromatic rings Agrobacterium tumefaciens C58 / ATCC 33970 ?
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 32000, SDS-PAGE Agrobacterium tumefaciens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
 Agrobacterium tumefaciens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Agrobacterium tumefaciens