Literature summary for 5.1.99.4 extracted from

Yevglevskis, M.; Lee, G.L.; Threadgill, M.D.; Woodman, T.J.; Lloyd, M.D.
The perils of rational design - unexpected irreversible elimination of fluoride from 3-fluoro-2-methylacyl-CoA esters catalysed by alpha-methylacyl-CoA racemase (AMACR; P504S) (2014), Chem. Commun. (Camb.), 50, 14164-14166 .

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KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.021	-	3-fluoro-2-methyldecanoyl-CoA	anti-enantiomer, pH not specified in the publication, temperature not specified in the publication	Homo sapiens
0.277	-	(R)-2-methyldecanoyl-CoA	pH not specified in the publication, temperature not specified in the publication	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(R)-2-methyldecanoyl-CoA	-	Homo sapiens	(S)-2-methyldecanoyl-CoA	-	?
3-fluoro-2-methyldecanoyl-CoA	-	Homo sapiens	E-2-methyldec-2-enoyl-CoA + HF	-	ir
additional information	rearrangement of the double bond of S- and R-E-2-methyldec-3-enoyl-CoA is not catalyzed	Homo sapiens	?	-	?

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.031	-	(R)-2-methyldecanoyl-CoA	pH not specified in the publication, temperature not specified in the publication	Homo sapiens
0.076	-	3-fluoro-2-methyldecanoyl-CoA	anti-enantiomer, pH not specified in the publication, temperature not specified in the publication	Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
0.112	-	(R)-2-methyldecanoyl-CoA	pH not specified in the publication, temperature not specified in the publication	Homo sapiens
3.61	-	3-fluoro-2-methyldecanoyl-CoA	anti-enantiomer, pH not specified in the publication, temperature not specified in the publication	Homo sapiens

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Release 2023.1 (January 2023)