Application | Comment | Organism |
---|---|---|
pharmacology | the enzyme is partially validated as a potential therapeutic target by siRNA knockdown of the AMACR gene, overview | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(24S,25R)-(3R,7R,12R)-trihydroxy-24-fluoro-5beta-cholestan-26-oyl-CoA | competitive inhibition | Rattus norvegicus | |
(2R,3S)-3-fluoro-2-methylhexadecanoyl-CoA | competitive inhibition | Rattus norvegicus | |
(2S,3R)-3-fluoro-2-methylhexadecanoyl-CoA | competitive inhibition | Rattus norvegicus | |
(R)-ibuprofenoyl-CoA | competitive inhibition | Rattus norvegicus | |
(S)-ibuprofenoyl-CoA | competitive inhibition | Rattus norvegicus | |
2-difluoromethylpentadecanoyl-CoA | competitive inhibition | Rattus norvegicus | |
2-methylmyristoyl CoA | competitive inhibition | Rattus norvegicus | |
2-methyloctanoyl CoA | competitive inhibition | Rattus norvegicus | |
2-trifluoromethyltetradecanoic acid | competitive inhibition | Rattus norvegicus | |
2-trifluoromethyltetradecanoyl-CoA | competitive inhibition | Rattus norvegicus | |
alpha-trifluoromethyltetradecanoic acid | inhibits growth of cancer cell lines PC3, CWR22 Rv1, and Du145 in a dose-dependent manner due to AMACR inhibition | Rattus norvegicus | |
beta-fluorinated branched chain alpha-methylacyl coenzyme A esters analogues | reversible competitive inhibitors, presence of beta-fluorine on the alpha-methyl group or the acyl chain results in a significant lowering of the Ki value compared with nonfluorinated analogs, and this is attributed to a lowering of the pKa of the alpha-proton, facilitating enolization and binding, competitive | Rattus norvegicus | |
additional information | design of mechanism-based irreversible inhibitors, 2-methylmyrist-2-enoyl CoA is not an inhibitor, mechanism, overview | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Rattus norvegicus | 5739 | - |
peroxisome | - |
Rattus norvegicus | 5777 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2S)-2-methylacyl-CoA | Rattus norvegicus | natural substrates are branched chain alpha-methylacyl coenzyme A esters | (2R)-2-methylacyl-CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(25S)-trihydroxycoprostanoyl-CoA | - |
Rattus norvegicus | (25R)-trihydroxycoprostanoyl-CoA | - |
? | |
(2S)-2-methylacyl-CoA | - |
Rattus norvegicus | (2R)-2-methylacyl-CoA | - |
? | |
(2S)-2-methylacyl-CoA | natural substrates are branched chain alpha-methylacyl coenzyme A esters | Rattus norvegicus | (2R)-2-methylacyl-CoA | - |
? | |
(S)-Pristanoyl-CoA | - |
Rattus norvegicus | (R)-Pristanoyl-CoA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
alpha-methylacyl-CoA racemase | - |
Rattus norvegicus |
AMACR | - |
Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Rattus norvegicus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | inhibition kinetics | Rattus norvegicus | |
0.0009 | - |
2-trifluoromethyltetradecanoyl-CoA | pH 7.0, 37°C | Rattus norvegicus | |
0.0013 | - |
(2R,3S)-3-fluoro-2-methylhexadecanoyl-CoA | pH 7.0, 37°C | Rattus norvegicus | |
0.0023 | - |
(2S,3R)-3-fluoro-2-methylhexadecanoyl-CoA | pH 7.0, 37°C | Rattus norvegicus | |
0.0038 | - |
(24S,25R)-(3R,7R,12R)-trihydroxy-24-fluoro-5beta-cholestan-26-oyl-CoA | pH 7.0, 37°C | Rattus norvegicus | |
0.0054 | - |
(R)-ibuprofenoyl-CoA | pH 7.0, 37°C | Rattus norvegicus | |
0.0192 | - |
(S)-ibuprofenoyl-CoA | pH 7.0, 37°C | Rattus norvegicus | |
0.02 | - |
2-difluoromethylpentadecanoyl-CoA | pH 7.0, 37°C | Rattus norvegicus | |
0.045 | - |
2-methyloctanoyl CoA | pH 7.0, 37°C | Rattus norvegicus | |
0.137 | - |
2-methylmyristoyl CoA | pH 7.0, 37°C | Rattus norvegicus |