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Literature summary for 5.1.3.B12 extracted from

  • Park, C.S.; Park, C.S.; Shin, K.C.; Oh, D.K.
    Production of d-psicose from D-fructose by whole recombinant cells with high-level expression of D-psicose 3-epimerase from Agrobacterium tumefaciens (2016), J. Biosci. Bioeng., 121, 186-190 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene agtu, subcloning in Escherichia coli strain ER2566, expression of recombinant His-tagged enzyme mutant I33L-S213C in Agrobacterium tumefaciens at high level, optimal at optimal at 60x02C, pH 8.5, 4 g/l cells, and 700 g/l D-fructose. The recombinant cells produced 230 g/l D-psicose after 40 min, with a conversion yield of 33% w/w, a volumetric productivity of 345 g/l/h, and a specific productivity of 86.2 g/g/h Agrobacterium tumefaciens

Protein Variants

Protein Variants Comment Organism
I33L-S213C site-directed mutagenesis, structure homology modeling and substrate docking the double-site variant I33L-S213C DPEase on the crystal structure of DPEase from Agrobacterium tumefaciens, PDB Id 2HK0, as a template. Production of D-psicose from D-fructose by whole recombinant cells and its crude enzyme under optimized conditions, overview Agrobacterium tumefaciens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-fructose Agrobacterium tumefaciens
-
D-psicose
-
r
D-fructose Agrobacterium tumefaciens C58 / ATCC 33970
-
D-psicose
-
r

Organism

Organism UniProt Comment Textmining
Agrobacterium tumefaciens A9CH28
-
-
Agrobacterium tumefaciens C58 / ATCC 33970 A9CH28
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Agrobacterium tumefaciens by nickel affinity chromatography, and dialysis Agrobacterium tumefaciens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-fructose
-
Agrobacterium tumefaciens D-psicose
-
r
D-fructose
-
Agrobacterium tumefaciens C58 / ATCC 33970 D-psicose
-
r

Subunits

Subunits Comment Organism
? x * 33000, recombinant His-tagged mutant enzyme, SDS-PAGE Agrobacterium tumefaciens

Synonyms

Synonyms Comment Organism
DPEase
-
Agrobacterium tumefaciens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
recombinant His-tagged mutant enzyme in vivo in recombinant cells Agrobacterium tumefaciens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50 55 half-lives of whole recombinant cells at 50°C and 55°C are 125 and 177% higher than those of its double-site variant enzyme, respectively. The thermostability of whole cells is higher than that of enzyme Agrobacterium tumefaciens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
recombinant His-tagged mutant enzyme in vivo in recombinant cells Agrobacterium tumefaciens

General Information

General Information Comment Organism
additional information structure homology modeling and substrate docking the double-site variant I33L-S213C DPEase based on the crystal structure of DPEase from Agrobacterium tumefaciens, PDB Id 2HK0, as a template Agrobacterium tumefaciens