Cloned (Comment) | Organism |
---|---|
gene agtu, subcloning in Escherichia coli strain ER2566, expression of recombinant His-tagged enzyme mutant I33L-S213C in Agrobacterium tumefaciens at high level, optimal at optimal at 60x02C, pH 8.5, 4 g/l cells, and 700 g/l D-fructose. The recombinant cells produced 230 g/l D-psicose after 40 min, with a conversion yield of 33% w/w, a volumetric productivity of 345 g/l/h, and a specific productivity of 86.2 g/g/h | Agrobacterium tumefaciens |
Protein Variants | Comment | Organism |
---|---|---|
I33L-S213C | site-directed mutagenesis, structure homology modeling and substrate docking the double-site variant I33L-S213C DPEase on the crystal structure of DPEase from Agrobacterium tumefaciens, PDB Id 2HK0, as a template. Production of D-psicose from D-fructose by whole recombinant cells and its crude enzyme under optimized conditions, overview | Agrobacterium tumefaciens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose | Agrobacterium tumefaciens | - |
D-psicose | - |
r | |
D-fructose | Agrobacterium tumefaciens C58 / ATCC 33970 | - |
D-psicose | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Agrobacterium tumefaciens | A9CH28 | - |
- |
Agrobacterium tumefaciens C58 / ATCC 33970 | A9CH28 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Agrobacterium tumefaciens by nickel affinity chromatography, and dialysis | Agrobacterium tumefaciens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose | - |
Agrobacterium tumefaciens | D-psicose | - |
r | |
D-fructose | - |
Agrobacterium tumefaciens C58 / ATCC 33970 | D-psicose | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 33000, recombinant His-tagged mutant enzyme, SDS-PAGE | Agrobacterium tumefaciens |
Synonyms | Comment | Organism |
---|---|---|
DPEase | - |
Agrobacterium tumefaciens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
recombinant His-tagged mutant enzyme in vivo in recombinant cells | Agrobacterium tumefaciens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 55 | half-lives of whole recombinant cells at 50°C and 55°C are 125 and 177% higher than those of its double-site variant enzyme, respectively. The thermostability of whole cells is higher than that of enzyme | Agrobacterium tumefaciens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
recombinant His-tagged mutant enzyme in vivo in recombinant cells | Agrobacterium tumefaciens |
General Information | Comment | Organism |
---|---|---|
additional information | structure homology modeling and substrate docking the double-site variant I33L-S213C DPEase based on the crystal structure of DPEase from Agrobacterium tumefaciens, PDB Id 2HK0, as a template | Agrobacterium tumefaciens |