Literature summary for 5.1.3.B12 extracted from

Chen, X.; Wang, W.; Xu, J.; Yuan, Z.; Yuan, T.; Zhang, Y.; Liang, C.; He, M.; Guo, Y.
Production of D-psicose from D-glucose by co-expression of D-psicose 3-epimerase and xylose isomerase (2017), Enzyme Microb. Technol., 105, 18-23 .

Search for more literature for 5.1.3.B12

Application

Application	Comment	Organism
synthesis	the enzyme from Agrobacterium tumefaciens can be used for production of D-psicose in a coexpression system with xylose isomerase gene from Escherichia coli. Method optimization and evaluation, overview	Agrobacterium tumefaciens

Cloned(Commentary)

Cloned (Comment)	Organism
DNA and amino acid sequence determination and analysis of the D-psicose 3-epimerase gene, recombinant expression in Escherichia coli strain BL21(DE3), co-expression with xylose isomerase gene from Escherichia coli strain MG1665 from plasmid pET-28a-DPE-xylA	Agrobacterium tumefaciens

Protein Variants

Protein Variants	Comment	Organism
additional information	for production of D-psicose from D-glucose in an enzymatic process, the xylose isomerase gene from Escherichia coli strain MG1665 and the D-psicose 3-epimerase gene from Agrobacterium tumefaciens CGMCC 1.1488 are coexpressed in Escherichia coli strain BL21(DE3). After 24 h incubation with the dual enzyme system at 40°C, the sugar conversion ratio from D-glucose to D-psicose reaches 10%. The optimal conditions are 50°C, pH 7.5 with Co2+ and Mg2+. The D-psicose yields from sugarcane bagasse and microalgae hydrolysate are 1.42 and 1.69 g/L, respectively. Co2+ is strictly required. Method optimization and evaluation, overview	Agrobacterium tumefaciens

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Co2+	highly activating, addition of Mg2+ to media containing Co2+ can further improve the conversion rate by 1.24fold, while Mg2+ alone is not activating	Agrobacterium tumefaciens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
D-fructose	Agrobacterium tumefaciens	-	D-psicose	-	r
D-fructose	Agrobacterium tumefaciens CGMCC 1.1488 / C58 / ATCC 33970	-	D-psicose	-	r
additional information	Agrobacterium tumefaciens	the xylose isomerase gene from Escherichia coli strain MG1665 and the D-psicose 3-epimerase gene from Agrobacterium tumefaciens CGMCC 1.1488 are coexpressed in Escherichia coli strain BL21(DE3). After 24 h incubation with the dual enzyme system at 40°C, the sugar conversion ratio from D-glucose to D-psicose reaches 10%. The optimal conditions are 50°C, pH 7.5 with Co2+ and Mg2+. The D-psicose yields from sugarcane bagasse and microalgae hydrolysate are 1.42 and 1.69 g/L, respectively	?	-	?
additional information	Agrobacterium tumefaciens CGMCC 1.1488 / C58 / ATCC 33970	the xylose isomerase gene from Escherichia coli strain MG1665 and the D-psicose 3-epimerase gene from Agrobacterium tumefaciens CGMCC 1.1488 are coexpressed in Escherichia coli strain BL21(DE3). After 24 h incubation with the dual enzyme system at 40°C, the sugar conversion ratio from D-glucose to D-psicose reaches 10%. The optimal conditions are 50°C, pH 7.5 with Co2+ and Mg2+. The D-psicose yields from sugarcane bagasse and microalgae hydrolysate are 1.42 and 1.69 g/L, respectively	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Agrobacterium tumefaciens	A9CH28	-	-
Agrobacterium tumefaciens CGMCC 1.1488 / C58 / ATCC 33970	A9CH28	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-fructose	-	Agrobacterium tumefaciens	D-psicose	-	r
D-fructose	-	Agrobacterium tumefaciens CGMCC 1.1488 / C58 / ATCC 33970	D-psicose	-	r
additional information	the xylose isomerase gene from Escherichia coli strain MG1665 and the D-psicose 3-epimerase gene from Agrobacterium tumefaciens CGMCC 1.1488 are coexpressed in Escherichia coli strain BL21(DE3). After 24 h incubation with the dual enzyme system at 40°C, the sugar conversion ratio from D-glucose to D-psicose reaches 10%. The optimal conditions are 50°C, pH 7.5 with Co2+ and Mg2+. The D-psicose yields from sugarcane bagasse and microalgae hydrolysate are 1.42 and 1.69 g/L, respectively	Agrobacterium tumefaciens	?	-	?
additional information	the xylose isomerase gene from Escherichia coli strain MG1665 and the D-psicose 3-epimerase gene from Agrobacterium tumefaciens CGMCC 1.1488 are coexpressed in Escherichia coli strain BL21(DE3). After 24 h incubation with the dual enzyme system at 40°C, the sugar conversion ratio from D-glucose to D-psicose reaches 10%. The optimal conditions are 50°C, pH 7.5 with Co2+ and Mg2+. The D-psicose yields from sugarcane bagasse and microalgae hydrolysate are 1.42 and 1.69 g/L, respectively	Agrobacterium tumefaciens CGMCC 1.1488 / C58 / ATCC 33970	?	-	?

Synonyms

Synonyms	Comment	Organism
DPEase	-	Agrobacterium tumefaciens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
40	-	assay at	Agrobacterium tumefaciens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	assay at	Agrobacterium tumefaciens

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Release 2023.1 (January 2023)