Cloned (Comment) | Organism |
---|---|
gene nanE, recombinant expression in Escherichia coli strain BL21(DE3) | Staphylococcus aureus |
Crystallization (Comment) | Organism |
---|---|
purified recombinant enzyme, sitting drop vapour diffusion method, mixing of 0.0015 ml of 10 mg/ml protein in 20 mM Tris-HCl, pH 8.0, with 0.0015 ml of crystallization solution containing 1 M trisodium citrate, 0.1 M sodium cacodylate, pH 6.5, 1 day, 8°C, X-ray diffraction structure determination and analysis at 1.84 A resolution, molecular replacement using monomeric enzyme structure, PDB ID 1y0e, as search template | Staphylococcus aureus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-acetyl-D-mannosamine-6-phosphate | Staphylococcus aureus | - |
N-acetyl-D-glucosamine-6-phosphate | - |
r | |
N-acetyl-D-mannosamine-6-phosphate | Staphylococcus aureus USA300 | - |
N-acetyl-D-glucosamine-6-phosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Staphylococcus aureus | A8YZE2 | methicillin-resistant | - |
Staphylococcus aureus USA300 | A8YZE2 | methicillin-resistant | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli strain BL21(DE3) by anion exchange and hydrophobic interaction chromatography, followed by gel filtration, and ultrafiltration | Staphylococcus aureus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-acetyl-D-mannosamine-6-phosphate | - |
Staphylococcus aureus | N-acetyl-D-glucosamine-6-phosphate | - |
r | |
N-acetyl-D-mannosamine-6-phosphate | - |
Staphylococcus aureus USA300 | N-acetyl-D-glucosamine-6-phosphate | - |
r |
Synonyms | Comment | Organism |
---|---|---|
N-acetylmannosamine-6-phosphate 2-epimerase | - |
Staphylococcus aureus |
nanE | - |
Staphylococcus aureus |
General Information | Comment | Organism |
---|---|---|
metabolism | the Nan-Nag cluster of genes encodes enzymes that scavenge sialic acid from the host, transport it into the bacterial cell and degrade it via a series of reactions into fructose-6-phosphate. This pathway has been documented in several bacterial pathogens. The N-acetylmannosamine-6-phosphate 2-epimerase NanE is part of the sialic acid catabolism | Staphylococcus aureus |
additional information | structure comparison with the enzyme structure, PDB ID 1y0e, overview | Staphylococcus aureus |