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Literature summary for 5.1.3.9 extracted from

  • North, R.; Kessans, S.; Griffin, M.; Watson, A.; Fairbanks, A.; Dobson, R.
    Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of N-acetylmannosamine-6-phosphate 2-epimerase from methicillin-resistant Staphylococcus aureus (2014), Acta Crystallogr. Sect. F, 70, 650-655 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene nanE, recombinant expression in Escherichia coli strain BL21(DE3) Staphylococcus aureus

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant enzyme, sitting drop vapour diffusion method, mixing of 0.0015 ml of 10 mg/ml protein in 20 mM Tris-HCl, pH 8.0, with 0.0015 ml of crystallization solution containing 1 M trisodium citrate, 0.1 M sodium cacodylate, pH 6.5, 1 day, 8°C, X-ray diffraction structure determination and analysis at 1.84 A resolution, molecular replacement using monomeric enzyme structure, PDB ID 1y0e, as search template Staphylococcus aureus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N-acetyl-D-mannosamine-6-phosphate Staphylococcus aureus
-
N-acetyl-D-glucosamine-6-phosphate
-
r
N-acetyl-D-mannosamine-6-phosphate Staphylococcus aureus USA300
-
N-acetyl-D-glucosamine-6-phosphate
-
r

Organism

Organism UniProt Comment Textmining
Staphylococcus aureus A8YZE2 methicillin-resistant
-
Staphylococcus aureus USA300 A8YZE2 methicillin-resistant
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Escherichia coli strain BL21(DE3) by anion exchange and hydrophobic interaction chromatography, followed by gel filtration, and ultrafiltration Staphylococcus aureus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-acetyl-D-mannosamine-6-phosphate
-
Staphylococcus aureus N-acetyl-D-glucosamine-6-phosphate
-
r
N-acetyl-D-mannosamine-6-phosphate
-
Staphylococcus aureus USA300 N-acetyl-D-glucosamine-6-phosphate
-
r

Synonyms

Synonyms Comment Organism
N-acetylmannosamine-6-phosphate 2-epimerase
-
Staphylococcus aureus
nanE
-
Staphylococcus aureus

General Information

General Information Comment Organism
metabolism the Nan-Nag cluster of genes encodes enzymes that scavenge sialic acid from the host, transport it into the bacterial cell and degrade it via a series of reactions into fructose-6-phosphate. This pathway has been documented in several bacterial pathogens. The N-acetylmannosamine-6-phosphate 2-epimerase NanE is part of the sialic acid catabolism Staphylococcus aureus
additional information structure comparison with the enzyme structure, PDB ID 1y0e, overview Staphylococcus aureus