Cloned (Comment) | Organism |
---|---|
gene age, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) at 37°C with coexpression of chaperones GroEL/GroES, 264fold increased AGE activity and maximum yield of 22.5 mg isolated AvaAGE per liter shake flask culture, compared to the starting conditions, method optimization, enhancement of soluble expression via chaperones, temperature dependence of soluble expression, and enhancement of soluble expression via fusion tags, overview | Trichormus variabilis |
Protein Variants | Comment | Organism |
---|---|---|
additional information | the majority of biocatalytic approaches for Neu5Ac synthesis involve an N-acylglucosamine 2-epimerase (AGE, EC 5.3.1.8) in combination with an N-acetylneuraminate lyase (NAL, EC 4.1.3.3) | Trichormus variabilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-Acyl-D-glucosamine | Trichormus variabilis | - |
N-Acyl-D-mannosamine | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trichormus variabilis | Q3M763 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) | Trichormus variabilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-Acyl-D-glucosamine | - |
Trichormus variabilis | N-Acyl-D-mannosamine | - |
r |
Synonyms | Comment | Organism |
---|---|---|
AGE | - |
Trichormus variabilis |
AvaAGE | - |
Trichormus variabilis |
N-acyl-D-glucosamine 2-epimerase | - |
Trichormus variabilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Trichormus variabilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Trichormus variabilis |
General Information | Comment | Organism |
---|---|---|
additional information | the majority of biocatalytic approaches for Neu5Ac synthesis involve an N-acylglucosamine 2-epimerase (AGE, EC 5.3.1.8) in combination with an N-acetylneuraminate lyase (NAL, EC 4.1.3.3) | Trichormus variabilis |
physiological function | N-acyl-D-glucosamine 2-epimerase (AGE) is an important enzyme for the biocatalytic synthesis of N-acetylneuraminic acid (Neu5Ac) | Trichormus variabilis |