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Literature summary for 5.1.3.8 extracted from

  • Kao, C.H.; Chen, Y.Y.; Wang, L.R.; Lee, Y.C.
    Production of N-acetyl-D-neuraminic acid by recombinant single whole cells co-expressing N-acetyl-D-glucosamine-2-epimerase and N-acetyl-D-neuraminic acid aldolase (2018), Mol. Biotechnol., 60, 427-434 .
    View publication on PubMed
Search for more literature for 5.1.3.8

Application

Application Comment Organism
synthesis recombinant Escherichia coli cells synchronously expressing N-acetyl-D-glucosamine-2-epimerase and N-acetyl-D-neuraminic acid aldolase as biocatalysts can potentially be used in the industrial mass production of Neu5Ac Anabaena sp. CH1

Cloned(Commentary)

Cloned (Comment) Organism
gene bage, recombinant expression of the His-tagged enzyme in Escherichia coli strain BL21(DE3), coexpression with N-acetyl-D-neuraminic acid aldolase gene nanA from Escherichia coli Anabaena sp. CH1

Protein Variants

Protein Variants Comment Organism
additional information production of N-acetyl-D-neuraminic acid by recombinant single whole cells co-expressing N-acetyl-D-glucosamine-2-epimerase and N-acetyl-D-neuraminic acid aldolase in Escherichia coli. Method development and evaluation, overview Anabaena sp. CH1

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N-acetyl-D-glucosamine Anabaena sp. CH1
-
 N-acetyl-D-mannosamine
-
 r
N-Acyl-D-glucosamine Anabaena sp. CH1
-
 N-Acyl-D-mannosamine
-
 r

Organism

Organism UniProt Comment Textmining
Anabaena sp. CH1 A4UA16
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, copurification with recombinant Escherichia coli N-acetyl-D-neuraminic acid aldolase Anabaena sp. CH1

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-acetyl-D-glucosamine
-
 Anabaena sp. CH1 N-acetyl-D-mannosamine
-
 r
N-Acyl-D-glucosamine
-
 Anabaena sp. CH1 N-Acyl-D-mannosamine
-
 r

Subunits

Subunits Comment Organism
? x * 42000, recombinant His-tagged enzyme, SDS-PAGE Anabaena sp. CH1

Synonyms

Synonyms Comment Organism
AGE
-
 Anabaena sp. CH1
bage
-
 Anabaena sp. CH1
GlcNAc-2-epimerase
-
 Anabaena sp. CH1
N-acetyl-D-glucosamine-2-epimerase
-
 Anabaena sp. CH1

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Anabaena sp. CH1

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Anabaena sp. CH1