Literature summary for 5.1.3.8 extracted from

Wang, Z.; Zhuang, W.; Cheng, J.; Sun, W.; Wu, J.; Chen, Y.; Ying, H.
In vivo multienzyme complex coconstruction of N-acetylneuraminic acid lyase and N-acetylglucosamine-2-epimerase for biosynthesis of N-acetylneuraminic acid (2017), J. Agric. Food Chem., 65, 7467-7475 .

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Cloned(Commentary)

Cloned (Comment)	Organism
recombinant enzyme expression in Escherichia coli, coexpression with N-acetylneuraminic acid lyase from Corynebacterium glutamicum ATCC 13032 (CgNal, EC 4.1.3.3)	Anabaena sp. CH1

Protein Variants

Protein Variants	Comment	Organism
additional information	metabolic channeling enables efficient transfer of the intermediates by forming a multienzyme complex. To leverage the metabolic channeling for improved biosynthesis, N-acetylneuraminic acid lyase from Corynebacterium glutamicum ATCC 13032 (CgNal, EC 4.1.3.3) and N-acetylglucosamine-2-epimerase from Anabaena sp. CH1 (anAGE) are coexpressed in Escherichia coli and the whole cell are used to synthesize N-acetylneuraminic acid (Neu5Ac) from N-acetylglucosamine (GlcNAc) and pyruvate. To get the multienzyme complex, a polycistronic plasmid with high levels of CgNal and anAGE expression is constructed by tuning the orders of the genes. The Shine-Dalgarno (SD) sequence and aligned spacing (AS) distance are optimized. The Escherichia coli strain Rosetta harboring the polycistronic plasmid pET-28a-SD2-AS1-CgNal-SD-AS-anAGE increases the production of Neu5Ac by 58.7% to 92.5 g/l in 36 h by whole-cell catalysis and by 21.9% up to 112.8 g/l in 24 h with the addition of Triton X-100	Anabaena sp. CH1

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
N-Acyl-D-glucosamine	Anabaena sp. CH1	-	N-Acyl-D-mannosamine	-	r

Organism

Organism	UniProt	Comment	Textmining
Anabaena sp. CH1	A4UA16	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
N-Acyl-D-glucosamine	-	Anabaena sp. CH1	N-Acyl-D-mannosamine	-	r

Synonyms

Synonyms	Comment	Organism
AGE	-	Anabaena sp. CH1
anAGE	-	Anabaena sp. CH1

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Release 2023.1 (January 2023)