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Literature summary for 5.1.3.6 extracted from

  • Molhoj, M.; Verma, R.; Reiter, W.D.
    The biosynthesis of D-galacturonate in plants. functional cloning and characterization of a membrane-anchored UDP-D-glucuronate 4-epimerase from Arabidopsis (2004), Plant Physiol., 135, 1221-1230.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Pichia pastoris Arabidopsis thaliana

General Stability

General Stability Organism
freezing and thawing of the crude extract in absence of cryoprotectants abolishes enzymatic activity entirely Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
additional information no inhibition: UDP, UDP-D-glucose or UDP-D-galactose Arabidopsis thaliana
UDP-D-Xyl strong Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.19
-
UDP-glucuronate
-
Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome exclusively localized in microsomal fraction, anchored to membrane Arabidopsis thaliana
-
-

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana O22141
-
-
Arabidopsis thaliana Q67ZJ4
-
-
Arabidopsis thaliana Q9M0B6
-
-
Arabidopsis thaliana Q9STI6
-
-

Source Tissue

Source Tissue Comment Organism Textmining
flower strong expression Arabidopsis thaliana
-
leaf strong expression Arabidopsis thaliana
-
leaf moderate expression Arabidopsis thaliana
-
additional information moderate expression throughout the plant Arabidopsis thaliana
-
additional information no expression in root or inflorescence Arabidopsis thaliana
-
root strong expression Arabidopsis thaliana
-
silique strong expression Arabidopsis thaliana
-
silique moderate expression Arabidopsis thaliana
-
stem strong expression Arabidopsis thaliana
-

Storage Stability

Storage Stability Organism
-20°C or -80°C in 50% v/v glycerol, 7 days, enzyme retains approximately 80% of its activity Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no epimerization of UDP-D-glucose and UDP-D-xylulose Arabidopsis thaliana ?
-
?
UDP-glucuronate
-
Arabidopsis thaliana UDP-D-galacturonate
-
?
UDP-glucuronate the recombinant enzyme establishes a 1.3:1 equilibrium between UDP-D-galacturonate and UDP-glucuronate Arabidopsis thaliana UDP-D-galacturonate
-
r

Synonyms

Synonyms Comment Organism
GAE1
-
Arabidopsis thaliana
GAE2
-
Arabidopsis thaliana
GAE3
-
Arabidopsis thaliana
GAE4
-
Arabidopsis thaliana
GAE5
-
Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
-
Arabidopsis thaliana

pH Range

pH Minimum pH Maximum Comment Organism
5 8.9 pH 5.0: about 75% of maximal activity, pH 8.9: 93% of maximal activity Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
NAD+ GAE1 expressed in Pichia pastoris already contains tightly bound NAD+ or NADP+ Arabidopsis thaliana
NADP+ GAE1 expressed in Pichia pastoris already contains tightly bound NAD+ or NADP+ Arabidopsis thaliana