Cloned (Comment) | Organism |
---|---|
expression in Pichia pastoris | Arabidopsis thaliana |
General Stability | Organism |
---|---|
freezing and thawing of the crude extract in absence of cryoprotectants abolishes enzymatic activity entirely | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | no inhibition: UDP, UDP-D-glucose or UDP-D-galactose | Arabidopsis thaliana | |
UDP-D-Xyl | strong | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.19 | - |
UDP-glucuronate | - |
Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | exclusively localized in microsomal fraction, anchored to membrane | Arabidopsis thaliana | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | O22141 | - |
- |
Arabidopsis thaliana | Q67ZJ4 | - |
- |
Arabidopsis thaliana | Q9M0B6 | - |
- |
Arabidopsis thaliana | Q9STI6 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
flower | strong expression | Arabidopsis thaliana | - |
leaf | strong expression | Arabidopsis thaliana | - |
leaf | moderate expression | Arabidopsis thaliana | - |
additional information | moderate expression throughout the plant | Arabidopsis thaliana | - |
additional information | no expression in root or inflorescence | Arabidopsis thaliana | - |
root | strong expression | Arabidopsis thaliana | - |
silique | strong expression | Arabidopsis thaliana | - |
silique | moderate expression | Arabidopsis thaliana | - |
stem | strong expression | Arabidopsis thaliana | - |
Storage Stability | Organism |
---|---|
-20°C or -80°C in 50% v/v glycerol, 7 days, enzyme retains approximately 80% of its activity | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | no epimerization of UDP-D-glucose and UDP-D-xylulose | Arabidopsis thaliana | ? | - |
? | |
UDP-glucuronate | - |
Arabidopsis thaliana | UDP-D-galacturonate | - |
? | |
UDP-glucuronate | the recombinant enzyme establishes a 1.3:1 equilibrium between UDP-D-galacturonate and UDP-glucuronate | Arabidopsis thaliana | UDP-D-galacturonate | - |
r |
Synonyms | Comment | Organism |
---|---|---|
GAE1 | - |
Arabidopsis thaliana |
GAE2 | - |
Arabidopsis thaliana |
GAE3 | - |
Arabidopsis thaliana |
GAE4 | - |
Arabidopsis thaliana |
GAE5 | - |
Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
- |
Arabidopsis thaliana |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 8.9 | pH 5.0: about 75% of maximal activity, pH 8.9: 93% of maximal activity | Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | GAE1 expressed in Pichia pastoris already contains tightly bound NAD+ or NADP+ | Arabidopsis thaliana | |
NADP+ | GAE1 expressed in Pichia pastoris already contains tightly bound NAD+ or NADP+ | Arabidopsis thaliana |