Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Azotobacter vinelandii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | 1 mM, complete loss of activity | Azotobacter vinelandii | |
NaCl | additions up to 0.1 M do not reduce the activity much, at 0.5 M NaCl less than 10% of the activity is retained | Azotobacter vinelandii | |
Zn2+ | 1.5 mM, complete inhibition | Azotobacter vinelandii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.018 | - |
[alginate]-beta-D-mannuronate | pH 6.8, 37°C | Azotobacter vinelandii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | required, optimum concentration 1-3 mM | Azotobacter vinelandii | |
Sr2+ | may substitute for Ca2+ with an efficiency of about 30% | Azotobacter vinelandii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
57700 | - |
x * 57700, calculated, x * 79000, SDS-PAGE | Azotobacter vinelandii |
79000 | - |
x * 57700, calculated, x * 79000, SDS-PAGE | Azotobacter vinelandii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Azotobacter vinelandii | Q44493 | - |
- |
Renatured (Comment) | Organism |
---|---|
epimerase activity of AlgE4 preincubated with 1 mM Na2EDTA can be restored by the addition of a molar excess of Ca2+ | Azotobacter vinelandii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme either slides along the alginate chain during catalysis or recognizes a pre-existing G residue as a preferred substrate in its consecutive attacks | Azotobacter vinelandii | ? | - |
? | |
[alginate]-beta-D-mannuronate | - |
Azotobacter vinelandii | [alginate]-alpha-L-guluronate | during epimerization of alginate, the fraction of GMG blocks increases linearly as a function of the total fraction of G residues and comparably much faster than that of MMG blocks | ? |
Subunits | Comment | Organism |
---|---|---|
? | x * 57700, calculated, x * 79000, SDS-PAGE | Azotobacter vinelandii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Azotobacter vinelandii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
stable up to | Azotobacter vinelandii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
14 | - |
[alginate]-beta-D-mannuronate | pH 6.8, 37°C | Azotobacter vinelandii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7 | - |
Azotobacter vinelandii |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8 | - |
complete loss of activity above | Azotobacter vinelandii |