Any feedback?
Literature summary for 5.1.3.37 extracted from
- Pseudomonas aeruginosa C5-mannuronan epimerase: steady-state kinetics and characterization of the product (2006), Biochemistry, 45, 552-560.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| erxpression in Escherichia coli | Pseudomonas aeruginosa |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | the Km decreases from 80 microM for substrate containing fewer than 15 residues to 4 microM for substrate containing over 100 residues, pH 7.3 | Pseudomonas aeruginosa |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no Ca2+ ions required for activity, and the Ca2+-alginate complex is not a substrate for the enzyme | Pseudomonas aeruginosa |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas aeruginosa | - |
- |
- |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -80 °C, 10% (v/v) glycerol, retains full catalytic activity for at least two months | Pseudomonas aeruginosa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the minimal substrate contains 9 monosaccharide residues. Tracts of adjacent guluronate residues are readily formed. The reaction reaches an apparent equilibrium when the guluronate composition of the polymer is 75% | Pseudomonas aeruginosa | ? | - |
? |  |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Pseudomonas aeruginosa |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | the maximum velocity of the reaction increases from 0.0018 per s to 0.0218 per s as the substrate size increases from 10 to 20 residues, and no additional increase in kcat is observed for substrates up to 100 residues in length, pH 7.3, temperature not specified in the publication | Pseudomonas aeruginosa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.3 | - |
assay at | Pseudomonas aeruginosa |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
