BRENDA - Enzyme Database show
show all sequences of 5.1.3.36

Heparosan-glucuronate 5-epimerase: molecular cloning and characterization of a novel enzyme

Mochizuki, H.; Yamagishi, K.; Suzuki, K.; Kim, Y.S.; Kimata, K.; Glycobiology 25, 735-744 (2015)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in insect cells
Achatina fulica
Inhibitors
Inhibitors
Commentary
Organism
Structure
CaCl2
-
Achatina fulica
CHAPS
0.1%, completely abolishes activity
Achatina fulica
MgCl2
-
Achatina fulica
MnCl2
-
Achatina fulica
NaCl
-
Achatina fulica
sodium deoxycholate
-
Achatina fulica
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
75000
-
x * 75000, SDS-PAGE
Achatina fulica
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
[heparosan]-D-glucuronate
Achatina fulica
it is suggested that the enzyme participates in the biosynthetic pathway of acharan sulfate
[heparosan]-L-iduronate
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Achatina fulica
A0A0C6EX43
-
-
Purification (Commentary)
Commentary
Organism
-
Achatina fulica
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
[heparosan]-D-glucuronate
it is suggested that the enzyme participates in the biosynthetic pathway of acharan sulfate
729809
Achatina fulica
[heparosan]-L-iduronate
-
-
-
?
[heparosan]-D-glucuronate
no activity with [heparosan]-N-sulfate D-glucuronate. At equilibrium of the epimerization, the proportion of IdoA in the reaction product reaches up to 30% of total hexuronic acid
729809
Achatina fulica
[heparosan]-L-iduronate
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 75000, SDS-PAGE
Achatina fulica
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Achatina fulica
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
35
-
unstable above
Achatina fulica
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
-
-
Achatina fulica
pH Range
pH Minimum
pH Maximum
Commentary
Organism
5.5
7
pH 5.0: about 50% of maximal activity, pH 7.0: about 40% of maximal activity
Achatina fulica
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in insect cells
Achatina fulica
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
CaCl2
-
Achatina fulica
CHAPS
0.1%, completely abolishes activity
Achatina fulica
MgCl2
-
Achatina fulica
MnCl2
-
Achatina fulica
NaCl
-
Achatina fulica
sodium deoxycholate
-
Achatina fulica
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
75000
-
x * 75000, SDS-PAGE
Achatina fulica
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
[heparosan]-D-glucuronate
Achatina fulica
it is suggested that the enzyme participates in the biosynthetic pathway of acharan sulfate
[heparosan]-L-iduronate
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Achatina fulica
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
[heparosan]-D-glucuronate
it is suggested that the enzyme participates in the biosynthetic pathway of acharan sulfate
729809
Achatina fulica
[heparosan]-L-iduronate
-
-
-
?
[heparosan]-D-glucuronate
no activity with [heparosan]-N-sulfate D-glucuronate. At equilibrium of the epimerization, the proportion of IdoA in the reaction product reaches up to 30% of total hexuronic acid
729809
Achatina fulica
[heparosan]-L-iduronate
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 75000, SDS-PAGE
Achatina fulica
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Achatina fulica
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
35
-
unstable above
Achatina fulica
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
-
-
Achatina fulica
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
5.5
7
pH 5.0: about 50% of maximal activity, pH 7.0: about 40% of maximal activity
Achatina fulica
Other publictions for EC 5.1.3.36
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
729809
Mochizuki
Heparosan-glucuronate 5-epimer ...
Achatina fulica
Glycobiology
25
735-744
2015
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1
-
-
-
6
-
-
-
1
1
-
2
-
-
1
-
-
-
-
-
2
1
1
-
1
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
6
-
-
-
-
1
1
-
-
-
1
-
-
-
-
2
1
1
-
1
-
1
1
-
-
-
-
-
-
-
-