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Literature summary for 5.1.3.36 extracted from
- Heparosan-glucuronate 5-epimerase: molecular cloning and characterization of a novel enzyme (2015), Glycobiology, 25, 735-744.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in insect cells | Lissachatina fulica |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| CaCl2 | - |
Lissachatina fulica |  |
| CHAPS | 0.1%, completely abolishes activity | Lissachatina fulica | |
| MgCl2 | - |
Lissachatina fulica | |
| MnCl2 | - |
Lissachatina fulica | |
| NaCl | - |
Lissachatina fulica | |
| sodium deoxycholate | - |
Lissachatina fulica | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 75000 | - |
x * 75000, SDS-PAGE | Lissachatina fulica |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| [heparosan]-D-glucuronate | Lissachatina fulica | it is suggested that the enzyme participates in the biosynthetic pathway of acharan sulfate | [heparosan]-L-iduronate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lissachatina fulica | A0A0C6EX43 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Lissachatina fulica |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| [heparosan]-D-glucuronate | it is suggested that the enzyme participates in the biosynthetic pathway of acharan sulfate | Lissachatina fulica | [heparosan]-L-iduronate | - |
? | |
| [heparosan]-D-glucuronate | no activity with [heparosan]-N-sulfate D-glucuronate. At equilibrium of the epimerization, the proportion of IdoA in the reaction product reaches up to 30% of total hexuronic acid | Lissachatina fulica | [heparosan]-L-iduronate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 75000, SDS-PAGE | Lissachatina fulica |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| HG-5epi | - |
Lissachatina fulica |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Lissachatina fulica |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
unstable above | Lissachatina fulica |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Lissachatina fulica |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 7 | pH 5.0: about 50% of maximal activity, pH 7.0: about 40% of maximal activity | Lissachatina fulica |
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Release 2023.1 (January 2023)
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