Data extracted from this reference:
Cloned(Commentary)
overexpression in Escherichia coli BL21(DE3)pLysS
Actinomyces sp.
Engineering
E151G
in the absence of 1,10-phenanthroline, the activity of the single mutant variant is similar to that of the wild type
Actinomyces sp.
E76G
in the absence of 1,10-phenanthroline, the activity of the single mutant variant is similar to that of the wild type
Actinomyces sp.
E76G/E151G
inactive mutant enzyme
Actinomyces sp.
E76G/H99G
inactive mutant enzyme
Actinomyces sp.
H14G
in the absence of 1,10-phenanthroline, the activity of the single mutant variant is similar to that of the wild type
Actinomyces sp.
H14G/E151G
inactive mutant enzyme
Actinomyces sp.
H14G/E76G
inactive mutant enzyme
Actinomyces sp.
H14G/H99G
inactive mutant enzyme
Actinomyces sp.
H99G
in the absence of 1,10-phenanthroline, the activity of the single mutant variant is similar to that of the wild type
Actinomyces sp.
H99G/E151G
inactive mutant enzyme
Actinomyces sp.
Metals/Ions
Co2+
can effectively rescue the activity of H99G mutant enzyme from which the metal ion has been removed with 1,10-phenanthroline
Actinomyces sp.
additional information
each monomer contains two beta,alpha,beta,beta,beta scaffolds that form a metal binding site with two histidine and two glutamic acid residues
Actinomyces sp.
Ni2+
can effectively rescue the activity of H99G mutant enzyme from which the metal ion has been removed with 1,10-phenanthroline
Actinomyces sp.
Molecular Weight [Da]
24000
2 * 24000, polyhistidine-tagged protein, SDS-PAGE
Actinomyces sp.
48000
polyhistidine-tagged protein, gel filtration, nondenaturing-PAGE
Actinomyces sp.
Natural Substrates/ Products (Substrates)
2-epi-5-epi-valiolone
Actinomyces sp.
the enzyme is involved in the biosynthesis of cetoniacytone A
5-epi-valiolone
?
2-epi-5-epi-valiolone
Actinomyces sp. Lu 9419
the enzyme is involved in the biosynthesis of cetoniacytone A
5-epi-valiolone
?
Organism
Actinomyces sp. Lu 9419
A1YPR3
Reaction
2-epi-5-epi-valiolone = 5-epi-valiolone
proposed reaction mechanism
Actinomyces sp.
Substrates and Products (Substrate)
2-epi-5-epi-valiolone
the enzyme is involved in the biosynthesis of cetoniacytone A
729590
Actinomyces sp.
5-epi-valiolone
?
2-epi-5-epi-valiolone
no activity with 1L-epi-2-inosose, D-(+)-gluconic acid delta-lactone, D-mannose, shikimic acid, dehydroshikimic acid, and aminodehydroshikimic acid
729590
Actinomyces sp.
5-epi-valiolone
?
2-epi-5-epi-valiolone
the enzyme is involved in the biosynthesis of cetoniacytone A
729590
Actinomyces sp. Lu 9419
5-epi-valiolone
?
2-epi-5-epi-valiolone
no activity with 1L-epi-2-inosose, D-(+)-gluconic acid delta-lactone, D-mannose, shikimic acid, dehydroshikimic acid, and aminodehydroshikimic acid
729590
Actinomyces sp. Lu 9419
5-epi-valiolone
?
Subunits
homodimer
2 * 24000, polyhistidine-tagged protein, SDS-PAGE
Actinomyces sp.
Synonyms
Temperature Optimum [°C]
30
assay at
Actinomyces sp.
pH Optimum
7.5
assay at
Actinomyces sp.
Cloned(Commentary) (protein specific)
overexpression in Escherichia coli BL21(DE3)pLysS
Actinomyces sp.
Engineering (protein specific)
E151G
in the absence of 1,10-phenanthroline, the activity of the single mutant variant is similar to that of the wild type
Actinomyces sp.
E76G
in the absence of 1,10-phenanthroline, the activity of the single mutant variant is similar to that of the wild type
Actinomyces sp.
E76G/E151G
inactive mutant enzyme
Actinomyces sp.
E76G/H99G
inactive mutant enzyme
Actinomyces sp.
H14G
in the absence of 1,10-phenanthroline, the activity of the single mutant variant is similar to that of the wild type
Actinomyces sp.
H14G/E151G
inactive mutant enzyme
Actinomyces sp.
H14G/E76G
inactive mutant enzyme
Actinomyces sp.
H14G/H99G
inactive mutant enzyme
Actinomyces sp.
H99G
in the absence of 1,10-phenanthroline, the activity of the single mutant variant is similar to that of the wild type
Actinomyces sp.
H99G/E151G
inactive mutant enzyme
Actinomyces sp.
Metals/Ions (protein specific)
Co2+
can effectively rescue the activity of H99G mutant enzyme from which the metal ion has been removed with 1,10-phenanthroline
Actinomyces sp.
additional information
each monomer contains two beta,alpha,beta,beta,beta scaffolds that form a metal binding site with two histidine and two glutamic acid residues
Actinomyces sp.
Ni2+
can effectively rescue the activity of H99G mutant enzyme from which the metal ion has been removed with 1,10-phenanthroline
Actinomyces sp.
Molecular Weight [Da] (protein specific)
24000
2 * 24000, polyhistidine-tagged protein, SDS-PAGE
Actinomyces sp.
48000
polyhistidine-tagged protein, gel filtration, nondenaturing-PAGE
Actinomyces sp.
Natural Substrates/ Products (Substrates) (protein specific)
2-epi-5-epi-valiolone
Actinomyces sp.
the enzyme is involved in the biosynthesis of cetoniacytone A
5-epi-valiolone
?
2-epi-5-epi-valiolone
Actinomyces sp. Lu 9419
the enzyme is involved in the biosynthesis of cetoniacytone A
5-epi-valiolone
?
Substrates and Products (Substrate) (protein specific)
2-epi-5-epi-valiolone
the enzyme is involved in the biosynthesis of cetoniacytone A
729590
Actinomyces sp.
5-epi-valiolone
?
2-epi-5-epi-valiolone
no activity with 1L-epi-2-inosose, D-(+)-gluconic acid delta-lactone, D-mannose, shikimic acid, dehydroshikimic acid, and aminodehydroshikimic acid
729590
Actinomyces sp.
5-epi-valiolone
?
2-epi-5-epi-valiolone
the enzyme is involved in the biosynthesis of cetoniacytone A
729590
Actinomyces sp. Lu 9419
5-epi-valiolone
?
2-epi-5-epi-valiolone
no activity with 1L-epi-2-inosose, D-(+)-gluconic acid delta-lactone, D-mannose, shikimic acid, dehydroshikimic acid, and aminodehydroshikimic acid
729590
Actinomyces sp. Lu 9419
5-epi-valiolone
?
Subunits (protein specific)
homodimer
2 * 24000, polyhistidine-tagged protein, SDS-PAGE
Actinomyces sp.
Temperature Optimum [°C] (protein specific)
30
assay at
Actinomyces sp.
pH Optimum (protein specific)
7.5
assay at
Actinomyces sp.
General Information
physiological function
the enzyme is involved in the biosynthesis of cetoniacytone A
Actinomyces sp.
General Information (protein specific)
physiological function
the enzyme is involved in the biosynthesis of cetoniacytone A
Actinomyces sp.
Other publictions for EC 5.1.3.33
729590
Wu
Biosynthetic gene cluster of c ...
Actinomyces sp., Actinomyces sp. Lu 9419
Chembiochem
10
304-314
2009
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