Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 5.1.3.31 extracted from

  • Itoh, H.; Okaya, H.; Khan, A. R.; Tajima, S.; Hayakawa, S.; Izumori, K.
    Purification and characterization of D-tagatose 3-epimerase from Pseudomonas sp. ST-24 (1994), Biosci. Biotechnol. Biochem., 58, 2168-2171.
No PubMed abstract available
Search for more literature for 5.1.3.31

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 1 mM, about 90% inhibition Pseudomonas sp.
Cu2+ 1 mM, about 60% inhibition Pseudomonas sp.
Hg2+ 1 mM, about 90% inhibition Pseudomonas sp.
p-chloromercuribenzoate 1 mM, about 90% inhibition Pseudomonas sp.
Zn2+ 1 mM, about 60% inhibition Pseudomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
55
-
 D-tagatose pH 7.5, 30°C Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33000
-
 2 * 33000, SDS-PAGE Pseudomonas sp.
68000
-
 gel filtration Pseudomonas sp.

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
-
-
Pseudomonas sp. ST-24
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas sp.

Source Tissue

Source Tissue Comment Organism Textmining
culture condition:D-glucose-grown cell cells grown on a medium containing 1% D-glucose and 0.05% D-tagatose Pseudomonas sp.
-
culture condition:D-tagatose-grown cell cells grown on a medium containing 1% D-glucose and 0.05% D-tagatose Pseudomonas sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
30
-
 pH 7.5, 30°C, substrate: D-tagatose Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-psicose epimerization of D-psicose at 60% compared to the activity with D-tagatose, epimerization of D-fructose at 20% compared to the activity with D-tagatose Pseudomonas sp. D-fructose
-
 r
D-psicose epimerization of D-psicose at 60% compared to the activity with D-tagatose, epimerization of D-fructose at 20% compared to the activity with D-tagatose Pseudomonas sp. ST-24 D-fructose
-
 r
D-ribulose epimerization of D-ribulose at 90% compared to the activity with D-tagatose, epimerization of D-xylulose at 40% compared to the activity with D-tagatose Pseudomonas sp. D-xylulose
-
 r
D-ribulose epimerization of D-ribulose at 90% compared to the activity with D-tagatose, epimerization of D-xylulose at 40% compared to the activity with D-tagatose Pseudomonas sp. ST-24 D-xylulose
-
 r
D-tagatose epimerization of D-sorbose at 20% compared to the activity with D-tagatose Pseudomonas sp. D-sorbose
-
 r
D-tagatose epimerization of D-sorbose at 20% compared to the activity with D-tagatose Pseudomonas sp. ST-24 D-sorbose
-
 r
L-ribulose epimerization of L-ribulose at 70% compared to the activity with D-tagatose, epimerization of L-xylulose at 20% compared to the activity with D-tagatose Pseudomonas sp. L-xylulose
-
 r
L-ribulose epimerization of L-ribulose at 70% compared to the activity with D-tagatose, epimerization of L-xylulose at 20% compared to the activity with D-tagatose Pseudomonas sp. ST-24 L-xylulose
-
 r
additional information no activity with D-fructose 6-phosphate and D-ribulose 5-phosphate Pseudomonas sp. ?
-
 ?
additional information no activity with D-fructose 6-phosphate and D-ribulose 5-phosphate Pseudomonas sp. ST-24 ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 33000, SDS-PAGE Pseudomonas sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Pseudomonas sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 10 min, stable Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 9 30°C Pseudomonas sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
7 11 30°C, 1 h, stable Pseudomonas sp.

pI Value

Organism Comment pI Value Maximum pI Value
Pseudomonas sp. isoelectric focusing
-
 4.6