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Literature summary for 5.1.3.30 extracted from

  • He, W.; Jiang, B.; Mu, W.; Zhang, T.
    Production of D-allulose with D-psicose 3-epimerase expressed and displayed on the surface of Bacillus subtilis spores (2016), J. Agric. Food Chem., 64, 7201-7207 .
    View publication on PubMed

Application

Application Comment Organism
synthesis D-psicose 3-epimerase is the key enzyme for producing D-allulose from inexpensive D-fructose sugar [Clostridium] scindens

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of D-psicose 3-epimerase and display on the surface of Bacillus subtilis spores. To improve the expression level of D-psicose 3-epimerase, the DPEase gene is fused with the promoter P43 to generate an expression cassette that introduced an identical cohesive end, and the resultant P43-DPE expression cassette is used as a monomeric fragment. The tandem repeats of the expression cassette are constructed by the isocaudamer method and integrated into the amyE gene locus of Bacillus subtilis by double-crossover. After DPEase is expressed in Bacillus subtilis, the antibiotic resistance gene is deleted via the Cre/lox system, thus generating food-grade strains [Clostridium] scindens

Protein Variants

Protein Variants Comment Organism
additional information to improve the expression level of D-psicose 3-epimerase, the DPEase gene is fused with the promoter P43 to generate an expression cassette that introduced an identical cohesive end, and the resultant P43-DPE expression cassette is used as a monomeric fragment. The tandem repeats of the expression cassette are constructed by the isocaudamer method and integrated into the amyE gene locus of Bacillus subtilis by double-crossover. After DPEase is expressed in Bacillus subtilis, the antibiotic resistance gene is deleted via the Cre/lox system, thus generating food-grade strains. D-Psicose (D-allulose) production by freeze-dried enzyme powder, the optimal substrate concentration is 300 g/L of D-fructose, generating 26.67 g/l/h of D-allulose yield with an 8.89% conversion rate [Clostridium] scindens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-psicose [Clostridium] scindens
-
D-fructose
-
r
D-psicose [Clostridium] scindens ATCC 35704
-
D-fructose
-
r

Organism

Organism UniProt Comment Textmining
[Clostridium] scindens
-
-
-
[Clostridium] scindens ATCC 35704
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-psicose
-
[Clostridium] scindens D-fructose
-
r
D-psicose
-
[Clostridium] scindens ATCC 35704 D-fructose
-
r

Synonyms

Synonyms Comment Organism
DPEase
-
[Clostridium] scindens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
assay at [Clostridium] scindens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at [Clostridium] scindens