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Literature summary for 5.1.3.3 extracted from

  • Sheshukova, E.V.; Komarova, T.V.; Pozdyshev, D.V.; Ershova, N.M.; Shindyapina, A.V.; Tashlitsky, V.N.; Sheval, E.V.; Dorokhov, Y.L.
    The intergenic interplay between aldose 1-epimerase-like protein and pectin methylesterase in abiotic and biotic stress control (2017), Front. Plant Sci., 8, 1646 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.1.3.3

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of a proNbAELP:GUS construct in Nicotinana benthamiana leaves via Agrobacterium tumefaciens strain GV3101 mediated transformation using the conventional leaf disc method, the NbAELP transcription promoter is sensitive to methanol, quantitive real-time PCR enzyme expression analysis Nicotiana benthamiana

Localization

Localization Comment Organism GeneOntology No. Textmining
cell wall primarily Nicotiana benthamiana 5618
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Organism

Organism UniProt Comment Textmining
Nicotiana benthamiana
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Source Tissue

Source Tissue Comment Organism Textmining
leaf very low enzyme level Nicotiana benthamiana
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additional information mRNA levels of the methanol-inducible gene encoding Nicotiana benthamiana aldose 1-epimerase-like protein (NbAELP) in the leaves of intact plants are very low compared with roots. Stress and pathogen attack increase the accumulation of the NbAELP mRNA in the leaves Nicotiana benthamiana
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root
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 Nicotiana benthamiana
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Synonyms

Synonyms Comment Organism
aldose 1-epimerase-like protein
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 Nicotiana benthamiana
NbAELP
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 Nicotiana benthamiana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
28 30 assay at Nicotiana benthamiana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Nicotiana benthamiana

Expression

Organism Comment Expression
Nicotiana benthamiana stress and pathogen attack increase the accumulation of the mRNA of methanol-inducible gene encoding Nicotiana benthamiana aldose 1-epimerase-like protein (NbAELP) in the leaves of intact plants up

General Information

General Information Comment Organism
evolution enzyme NbAELP belongs to the aldose 1-epimerases, classified in the family of mutarotases Nicotiana benthamiana
malfunction knockdown of enzyme NbAELP results in high activity of pectin methylesterase (PME) in the cell wall and a decrease in the leaf glucose level, creating unfavorable conditions for Agrobacterium tumefaciens reproduction in injected leaves. NbAELP gene knockdown followed by NbPME overexpression leads to dwarfism and leaf structure modifications Nicotiana benthamiana
metabolism the mechanical damage that often precedes the penetration of a leaf by a pathogen promotes the activation of pectin methylesterase (PME). The activation of PME leads to the emission of methanol, resulting in a priming effect on intact leaves, which is accompanied by an increased sensitivity to Tobacco mosaic virus (TMV) and resistance to bacteria, e.g. by induction of enzyme NbAELP Nicotiana benthamiana
physiological function methanol-inducible NbAELP is capable of binding the Tobacco mosaic virus (TMV) movement protein (MPTMV) in vitro and is likely to affect the cellular nucleocytoplasmic transport, which may explain the sensitivity of NbAELP knockdown plants to TMV. Although NbAELP is primarily detected in the cell wall, the influence of this protein on cellular PME mRNA levels might be associated with reduced transcriptional activity of the pectin methylesterase (PME) gene in the nucleus. Plant wounding and/or pathogen attack lead to PME activation and increased methanol emission, followed by increased NbAELP expression, which results in reversion of PME mRNA level and methanol emission to levels found in the intact plant. NbAELP downregulates mRNA accumulation directed by PME transcription promoter and downregulates the synthesis of PME mRNA by the feedback principle Nicotiana benthamiana