Literature summary for 5.1.3.3 extracted from

Chittori, S.; Simanshu, D.K.; Savithri, H.S.; Murthy, M.R.
Structure of the putative mutarotase YeaD from Salmonella typhimurium: structural comparison with galactose mutarotases (2007), Acta Crystallogr. Sect. D, 63, 197-205.

Cloned(Commentary)

Cloned (Comment)	Organism
cloned into pRSET-C vector (Invitrogen), expression in Escherichia coli	Salmonella enterica
gene yeaD, expression of the His-tagged enzyme in Escherichia coli strain BL21(DE3)	Salmonella enterica subsp. enterica serovar Typhimurium
overexpression in Escherichia coli	Salmonella enterica subsp. enterica serovar Typhimurium

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging-drop vapour-diffusion method	Salmonella enterica subsp. enterica serovar Typhimurium
purified recombinant His-tagged enzyme, hanging-drop vapour-diffusion method, 0.003 ml of 10 mg/ml protein in 15-25% PEG 8000, 0.1 M Na citrate, pH 5.5, and 0.2-0.5 mM NaCl for monoclinic crystals, or in 1.5-2 M ammonium sulfate, 25 mM Mg formate, 0.1 M Na cacodylate, pH 5.5, for orthorhombic crystals, cocrystallization of YeaD with ligands glucose, galactose, mannose, fructose, fucose, xylose, lyxose, lactose, maltose, sucrose and glucose 6-phosphate, incubation at 4°C for 12 h in a 1:10 molar ratio, X-ray diffraction structure determination and analysis the orthorhombic form at 1.9 A resolution and in the monoclinic form at 2.5 A resolution	Salmonella enterica subsp. enterica serovar Typhimurium

Crystallization (Comment)

Organism

hanging-drop vapour-diffusion method

Salmonella enterica subsp. enterica serovar Typhimurium

purified recombinant His-tagged enzyme, hanging-drop vapour-diffusion method, 0.003 ml of 10 mg/ml protein in 15-25% PEG 8000, 0.1 M Na citrate, pH 5.5, and 0.2-0.5 mM NaCl for monoclinic crystals, or in 1.5-2 M ammonium sulfate, 25 mM Mg formate, 0.1 M Na cacodylate, pH 5.5, for orthorhombic crystals, cocrystallization of YeaD with ligands glucose, galactose, mannose, fructose, fucose, xylose, lyxose, lactose, maltose, sucrose and glucose 6-phosphate, incubation at 4°C for 12 h in a 1:10 molar ratio, X-ray diffraction structure determination and analysis the orthorhombic form at 1.9 A resolution and in the monoclinic form at 2.5 A resolution

Salmonella enterica subsp. enterica serovar Typhimurium

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
34000	-	SDS-PAGE	Salmonella enterica subsp. enterica serovar Typhimurium
34000	-	recombinant His-tagged enzyme, gel filtration	Salmonella enterica subsp. enterica serovar Typhimurium

Organism

Organism	UniProt	Comment	Textmining
Salmonella enterica	-	-	-
Salmonella enterica subsp. enterica serovar Typhimurium	-	-	-
Salmonella enterica subsp. enterica serovar Typhimurium	-	gene yeaD	-

Purification (Commentary)

Purification (Comment)	Organism
NiNTA affinity chromatography	Salmonella enterica subsp. enterica serovar Typhimurium
NiNTA affinity column chromatography	Salmonella enterica
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography	Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	identification of residues involved in catalysis and substrate binding, role of Arg74 and Arg99, found only in YeaD-like proteins, in ligand anchoring, the specificity of YeaD is different from those of galactose mutarotase, overview	Salmonella enterica subsp. enterica serovar Typhimurium	?	-	?

Subunits

Subunits	Comment	Organism
monomer	-	Salmonella enterica subsp. enterica serovar Typhimurium
monomer	1 x 34000, recombinant His-tagged enzyme, SDS-PAGE, the enzyme is a monomer in solution, but two monomers A and B related by a noncrystallographic twofold axis are found in the asymmetric unit of the orthorhombic form, while two pairs of such monomers (AB and CD) are observed in the monoclinic crystal form, overview	Salmonella enterica subsp. enterica serovar Typhimurium
More	YeaD adopts the beta-sandwich fold, sugar ligand binding structure, overview	Salmonella enterica subsp. enterica serovar Typhimurium

Synonyms

Synonyms	Comment	Organism
aldose 1-epimerase	-	Salmonella enterica subsp. enterica serovar Typhimurium
aldose 1-epimerase	-	Salmonella enterica
Mutarotase	-	Salmonella enterica subsp. enterica serovar Typhimurium
mutarotase YeaD	-	Salmonella enterica subsp. enterica serovar Typhimurium
mutarotase YeaD	-	Salmonella enterica
stYeaD	-	Salmonella enterica subsp. enterica serovar Typhimurium
YeaD	-	Salmonella enterica subsp. enterica serovar Typhimurium