BRENDA - Enzyme Database show
show all sequences of 5.1.3.28

Biosynthesis of UDP-N-acetyl-L-fucosamine, a precursor to the biosynthesis of lipopolysaccharide in Pseudomonas aeruginosa serotype O11

Mulrooney, E.F.; Poon, K.K.; McNally, D.J.; Brisson, J.R.; Lam, J.S.; J. Biol. Chem. 280, 19535-19542 (2005)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
overexpression in Escherichia coli
Pseudomonas aeruginosa
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
UDP-2-acetamido-2,6-dideoxy-beta-L-talose
Pseudomonas aeruginosa
the enzyme is involved in bacterial polysaccharide biosynthesis
UDP-N-acetyl-beta-L-fucosamine
-
-
?
UDP-2-acetamido-2,6-dideoxy-beta-L-talose
Pseudomonas aeruginosa serotype O11
the enzyme is involved in bacterial polysaccharide biosynthesis
UDP-N-acetyl-beta-L-fucosamine
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas aeruginosa
Q9XC59
-
-
Pseudomonas aeruginosa serotype O11
Q9XC59
-
-
Purification (Commentary)
Commentary
Organism
-
Pseudomonas aeruginosa
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
UDP-2-acetamido-2,6-dideoxy-beta-L-talose
the enzyme is involved in bacterial polysaccharide biosynthesis
725364
Pseudomonas aeruginosa
UDP-N-acetyl-beta-L-fucosamine
-
-
-
?
UDP-2-acetamido-2,6-dideoxy-beta-L-talose
UDP-2-acetamido-2,6-dideoxy-beta-L-talose i.e. UDP-N-acetyl-beta-L-pneumosamine. At pH 9.0, the C-2 epimerization reaction catalyzed by WbjD favors the production of UDP-N-acetyl-beta-L-fucosamine over UDP-2-acetamido-2,6-dideoxy-beta-L-talose
725364
Pseudomonas aeruginosa
UDP-N-acetyl-beta-L-fucosamine
-
-
-
r
UDP-2-acetamido-2,6-dideoxy-beta-L-talose
the enzyme is involved in bacterial polysaccharide biosynthesis
725364
Pseudomonas aeruginosa serotype O11
UDP-N-acetyl-beta-L-fucosamine
-
-
-
?
UDP-2-acetamido-2,6-dideoxy-beta-L-talose
UDP-2-acetamido-2,6-dideoxy-beta-L-talose i.e. UDP-N-acetyl-beta-L-pneumosamine. At pH 9.0, the C-2 epimerization reaction catalyzed by WbjD favors the production of UDP-N-acetyl-beta-L-fucosamine over UDP-2-acetamido-2,6-dideoxy-beta-L-talose
725364
Pseudomonas aeruginosa serotype O11
UDP-N-acetyl-beta-L-fucosamine
-
-
-
r
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
37
-
assay at
Pseudomonas aeruginosa
Cloned(Commentary) (protein specific)
Commentary
Organism
overexpression in Escherichia coli
Pseudomonas aeruginosa
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
UDP-2-acetamido-2,6-dideoxy-beta-L-talose
Pseudomonas aeruginosa
the enzyme is involved in bacterial polysaccharide biosynthesis
UDP-N-acetyl-beta-L-fucosamine
-
-
?
UDP-2-acetamido-2,6-dideoxy-beta-L-talose
Pseudomonas aeruginosa serotype O11
the enzyme is involved in bacterial polysaccharide biosynthesis
UDP-N-acetyl-beta-L-fucosamine
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Pseudomonas aeruginosa
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
UDP-2-acetamido-2,6-dideoxy-beta-L-talose
the enzyme is involved in bacterial polysaccharide biosynthesis
725364
Pseudomonas aeruginosa
UDP-N-acetyl-beta-L-fucosamine
-
-
-
?
UDP-2-acetamido-2,6-dideoxy-beta-L-talose
UDP-2-acetamido-2,6-dideoxy-beta-L-talose i.e. UDP-N-acetyl-beta-L-pneumosamine. At pH 9.0, the C-2 epimerization reaction catalyzed by WbjD favors the production of UDP-N-acetyl-beta-L-fucosamine over UDP-2-acetamido-2,6-dideoxy-beta-L-talose
725364
Pseudomonas aeruginosa
UDP-N-acetyl-beta-L-fucosamine
-
-
-
r
UDP-2-acetamido-2,6-dideoxy-beta-L-talose
the enzyme is involved in bacterial polysaccharide biosynthesis
725364
Pseudomonas aeruginosa serotype O11
UDP-N-acetyl-beta-L-fucosamine
-
-
-
?
UDP-2-acetamido-2,6-dideoxy-beta-L-talose
UDP-2-acetamido-2,6-dideoxy-beta-L-talose i.e. UDP-N-acetyl-beta-L-pneumosamine. At pH 9.0, the C-2 epimerization reaction catalyzed by WbjD favors the production of UDP-N-acetyl-beta-L-fucosamine over UDP-2-acetamido-2,6-dideoxy-beta-L-talose
725364
Pseudomonas aeruginosa serotype O11
UDP-N-acetyl-beta-L-fucosamine
-
-
-
r
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
37
-
assay at
Pseudomonas aeruginosa
General Information
General Information
Commentary
Organism
physiological function
the enzyme is involved in bacterial polysaccharide biosynthesis
Pseudomonas aeruginosa
General Information (protein specific)
General Information
Commentary
Organism
physiological function
the enzyme is involved in bacterial polysaccharide biosynthesis
Pseudomonas aeruginosa
Other publictions for EC 5.1.3.28
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
725364
Mulrooney
Biosynthesis of UDP-N-acetyl-L ...
Pseudomonas aeruginosa, Pseudomonas aeruginosa serotype O11
J. Biol. Chem.
280
19535-19542
2005
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1
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726897
Kneidinger
Biosynthesis of 2-acetamido-2, ...
Pseudomonas aeruginosa
Biochem. J.
371
989-995
2003
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727843
Kneidinger
Three highly conserved protein ...
Pseudomonas aeruginosa, Pseudomonas aeruginosa O11, Staphylococcus aureus, Staphylococcus aureus Newman
J. Biol. Chem.
278
3615-3627
2003
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2
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14
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