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Literature summary for 5.1.3.23 extracted from
- Identification and biochemical characterization of two novel UDP-2,3-diacetamido-2,3-dideoxy-alpha -D-glucuronic acid 2-epimerases from respiratory pathogens (2007), Biochem. J., 405, 123-130.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| His6-WbpI is overexpressed in Escherichia coli RosettaTM cells | Pseudomonas aeruginosa |
| His6WlbD is overexpressed in Escherichia coli RosettaTM cells | Bordetella pertussis |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40300 | - |
x * 40300, His6-WbpI, SDS-PAGE | Pseudomonas aeruginosa |
| 42600 | - |
x * 42600, His6WlbD, SDS-PAGE | Bordetella pertussis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-2,3-diacetamido-2,3-dideoxy-alpha-D-glucuronate | Pseudomonas aeruginosa | the enzyme participates in the biosynthetic pathway for UDP-alpha-D-ManNAc3NAcA (UDP-2,3-diacetamido-2,3-dideoxy-alpha-D-mannuronic acid), an important precursor of the B-band lipopolysaccharide of Pseudomonas aeruginosa serotype O5 | UDP-2,3-diacetamido-2,3-dideoxy-alpha-D-mannuronate | - |
? |  |
| UDP-2,3-diacetamido-2,3-dideoxy-alpha-D-glucuronate | Bordetella pertussis | the enzyme participates in the the biosynthetic pathway for band-A trisaccharide of Bordetella pertussis | UDP-2,3-diacetamido-2,3-dideoxy-alpha-D-mannuronate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bordetella pertussis | - |
- |
- |
| Pseudomonas aeruginosa | - |
serogroup O5 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant | Pseudomonas aeruginosa |
| recombinant | Bordetella pertussis |
Storage Stability
| Storage Stability | Organism |
|---|---|
| His6-WbpI stored at -20°C in the presence of 25% glycerol retains activity for at least 4 weeks | Pseudomonas aeruginosa |
| His6-WlbD stored at -20°C in the presence of 25% glycerol retains activity for at least 4 weeks | Bordetella pertussis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-2,3-diacetamido-2,3-dideoxy-alpha-D-glucuronate | the enzyme participates in the biosynthetic pathway for UDP-alpha-D-ManNAc3NAcA (UDP-2,3-diacetamido-2,3-dideoxy-alpha-D-mannuronic acid), an important precursor of the B-band lipopolysaccharide of Pseudomonas aeruginosa serotype O5 | Pseudomonas aeruginosa | UDP-2,3-diacetamido-2,3-dideoxy-alpha-D-mannuronate | - |
? | |
| UDP-2,3-diacetamido-2,3-dideoxy-alpha-D-glucuronate | the enzyme participates in the the biosynthetic pathway for band-A trisaccharide of Bordetella pertussis | Bordetella pertussis | UDP-2,3-diacetamido-2,3-dideoxy-alpha-D-mannuronate | - |
? | |
| UDP-2,3-diacetamido-2,3-dideoxy-alpha-D-glucuronate | he enzyme is highly specific as UDP-alpha-D-GlcNAc, UDP-alpha-D-GlcNAcA (UDP-2-acetamido-2-deoxy-alpha-D-glucuronic acid) and UDP-alpha-D-GlcNAc3NAc (UDP-2,3-diacetamido-2,3-dideoxy-alpha-D-glucose) cannot act as substrates | Bordetella pertussis | UDP-2,3-diacetamido-2,3-dideoxy-alpha-D-mannuronate | - |
? | |
| UDP-2,3-diacetamido-2,3-dideoxy-alpha-D-glucuronate | the enzyme is highly specific as UDP-alpha-D-GlcNAc, UDP-alpha-D-GlcNAcA (UDP-2-acetamido-2-deoxy-alpha-D-glucuronic acid) and UDP-alpha-D-GlcNAc3NAc (UDP-2,3-diacetamido-2,3-dideoxy-alpha-D-glucose) cannot act as substrates | Pseudomonas aeruginosa | UDP-2,3-diacetamido-2,3-dideoxy-alpha-D-mannuronate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 40300, His6-WbpI, SDS-PAGE | Pseudomonas aeruginosa |
| ? | x * 42600, His6WlbD, SDS-PAGE | Bordetella pertussis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 2,3-diacetamido-2,3-dideoxy-alpha-D-glucuronic acid 2-epimerase | - |
Pseudomonas aeruginosa |
| 2,3-diacetamido-2,3-dideoxy-alpha-D-glucuronic acid 2-epimerase | - |
Bordetella pertussis |
| UDP-2,3-diacetamido-2,3-dideoxy-alpha-D-glucuronic acid 2-epimerases | - |
Pseudomonas aeruginosa |
| UDP-2,3-diacetamido-2,3-dideoxy-alpha-D-glucuronic acid 2-epimerases | - |
Bordetella pertussis |
| UDP-alpha-D-GlcNAc3NAcA 2-epimerase | - |
Pseudomonas aeruginosa |
| UDP-alpha-D-GlcNAc3NAcA 2-epimerase | - |
Bordetella pertussis |
| UDP-GlcNAc3NAcA 2-epimerase | - |
Pseudomonas aeruginosa |
| UDP-GlcNAc3NAcA 2-epimerase | - |
Bordetella pertussis |
| WbpI | - |
Pseudomonas aeruginosa |
| WlbD | - |
Bordetella pertussis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 37 | - |
Pseudomonas aeruginosa |
| 30 | 37 | - |
Bordetella pertussis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Pseudomonas aeruginosa |
| 6 | - |
- |
Bordetella pertussis |
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