BRENDA - Enzyme Database show
show all sequences of 5.1.3.21

A novel enzyme, maltose 1-epimerase from Lactobacillus brevis IFO 3345

Shirokane, Y.; Suzuki, M.; FEBS Lett. 367, 177-179 (1995)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
Cu2+
-
Lactobacillus brevis
Hg2+
-
Lactobacillus brevis
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.2
-
beta-maltose
-
Lactobacillus brevis
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
43000
-
gel filtration
Lactobacillus brevis
45000
-
1 * 45000, SDS-PAGE
Lactobacillus brevis
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Lactobacillus brevis
-
IFO 3345
-
Purification (Commentary)
Commentary
Organism
-
Lactobacillus brevis
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1710
-
-
Lactobacillus brevis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
alpha-D-glucose
at 26% of the activity with beta-maltose
492266
Lactobacillus brevis
beta-D-glucose
-
-
-
r
alpha-lactose
at 5% of the activity with beta-maltose
492266
Lactobacillus brevis
beta-lactose
-
-
-
?
beta-cellobiose
at 4% of the activity with beta-maltose
492266
Lactobacillus brevis
alpha-cellobiose
-
-
-
?
beta-D-glucose
at 27% of the activity with beta-maltose
492266
Lactobacillus brevis
alpha-D-glucose
-
-
-
r
beta-Maltose
-
492266
Lactobacillus brevis
alpha-Maltose
-
492266
Lactobacillus brevis
r
Subunits
Subunits
Commentary
Organism
monomer
1 * 45000, SDS-PAGE
Lactobacillus brevis
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
40
-
-
Lactobacillus brevis
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
45
-
10 min, 50 mM phosphate buffer, stable
Lactobacillus brevis
60
-
10 min, 50 mM phosphate buffer, 50% loss of activity
Lactobacillus brevis
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
7
-
Lactobacillus brevis
pH Range
pH Minimum
pH Maximum
Commentary
Organism
5
8.5
more than 80% of activity in the pH-range pH 5.0-8.5
Lactobacillus brevis
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Cu2+
-
Lactobacillus brevis
Hg2+
-
Lactobacillus brevis
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.2
-
beta-maltose
-
Lactobacillus brevis
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
43000
-
gel filtration
Lactobacillus brevis
45000
-
1 * 45000, SDS-PAGE
Lactobacillus brevis
Purification (Commentary) (protein specific)
Commentary
Organism
-
Lactobacillus brevis
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1710
-
-
Lactobacillus brevis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
alpha-D-glucose
at 26% of the activity with beta-maltose
492266
Lactobacillus brevis
beta-D-glucose
-
-
-
r
alpha-lactose
at 5% of the activity with beta-maltose
492266
Lactobacillus brevis
beta-lactose
-
-
-
?
beta-cellobiose
at 4% of the activity with beta-maltose
492266
Lactobacillus brevis
alpha-cellobiose
-
-
-
?
beta-D-glucose
at 27% of the activity with beta-maltose
492266
Lactobacillus brevis
alpha-D-glucose
-
-
-
r
beta-Maltose
-
492266
Lactobacillus brevis
alpha-Maltose
-
492266
Lactobacillus brevis
r
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 45000, SDS-PAGE
Lactobacillus brevis
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
40
-
-
Lactobacillus brevis
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
45
-
10 min, 50 mM phosphate buffer, stable
Lactobacillus brevis
60
-
10 min, 50 mM phosphate buffer, 50% loss of activity
Lactobacillus brevis
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
7
-
Lactobacillus brevis
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
5
8.5
more than 80% of activity in the pH-range pH 5.0-8.5
Lactobacillus brevis
Other publictions for EC 5.1.3.21
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
492266
Shirokane
A novel enzyme, maltose 1-epim ...
Lactobacillus brevis
FEBS Lett.
367
177-179
1995
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2
1
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2
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2
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1
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1
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5
1
1
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2
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1
1
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2
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1
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2
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1
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1
-
5
1
1
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2
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1
1
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