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Literature summary for 5.1.3.20 extracted from

  • Kowatz, T.; Morrison, J.P.; Tanner, M.E.; Naismith, J.H.
    The crystal structure of the Y140F mutant of ADP-L-glycero-D-manno-heptose 6-epimerase bound to ADP-beta-D-mannose suggests a one base mechanism (2010), Protein Sci., 19, 1337-1343.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.1.3.20

Cloned(Commentary)

Cloned (Comment) Organism
mutant enzyme Y140F is expressed in Escherichia coli BL21(DE3) cells Escherichia coli

Crystallization (Commentary)

Crystallization (Comment) Organism
Y140F mutant enzyme bound to ADP-beta-D-mannose, hanging drop vapor diffusion method, using 2M (NH4)2SO4, 0.1 M HEPES-Na, pH 7.1, 2% (w7v) PEG 400 Escherichia coli

Protein Variants

Protein Variants Comment Organism
Y140F the mutant shows 0.08% epimerase activity compared to the wild type enzyme Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P67910
-
-

Purification (Commentary)

Purification (Comment) Organism
metal affinity column chromatography, hydrophobic affinity column chromatography, and gel filtration Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP-D-glycero-D-mannoheptose
-
 Escherichia coli ADP-L-glycero-D-mannoheptose
-
 ?

Subunits

Subunits Comment Organism
pentamer x-ray crystallography Escherichia coli

Synonyms

Synonyms Comment Organism
ADP-L-glycero-D-manno-heptose 6-epimerase
-
 Escherichia coli
AGME
-
 Escherichia coli

Cofactor

Cofactor Comment Organism Structure
NAD+ AGME preferentially utilizes NADP+ but can use NAD+ (lower activity) Escherichia coli
NADP+ AGME preferentially utilizes NADP+ Escherichia coli