Literature summary for 5.1.3.20 extracted from

Morrison, J.P.; Tanner, M.E.
A Two-Base Mechanism for Escherichia coli ADP-L-glycero-D-manno-Heptose 6-Epimerase (2007), Biochemistry, 46, 3916-3924.

Search for more literature for 5.1.3.20

Cloned(Commentary)

Cloned (Comment)	Organism
Plasmids encoding hexahistidine-tagged mutant and wildtype enzymes overexpressed in Escherichia coli.	Escherichia coli

Protein Variants

Protein Variants	Comment	Organism
D210N	mutant display activity similar to that of the wild type	Escherichia coli
K178M	mutant has severely compromised epimerase activities that are more than 3 orders of magnitude lower than that of the wild type	Escherichia coli
K208M	mutant display activity similar to that of the wild type	Escherichia coli
Y140F	mutant has severely compromised epimerase activities that are more than 3 orders of magnitude lower than that of the wild type	Escherichia coli

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.1	-	ADP-D-glycero-D-manno-heptose	NMR spectroscopy	Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ADP-D-glycero-D-manno-heptose	Escherichia coli	biosynthetic pathway of L-glycero-D-manno-heptose	ADP-L-glycero-D-manno-heptose	-	r

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
2.7	-	at 23°C	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ADP-D-glycero-D-manno-heptose	biosynthetic pathway of L-glycero-D-manno-heptose	Escherichia coli	ADP-L-glycero-D-manno-heptose	-	r

Synonyms

Synonyms	Comment	Organism
ADP-L-glycero-D-manno-heptose 6-epimerase	-	Escherichia coli
AGME	formerly RfaD	Escherichia coli
hldD	-	Escherichia coli

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Release 2023.1 (January 2023)