Literature summary for 5.1.3.2 extracted from

Lee, M.J.; Gravelat, F.N.; Cerone, R.P.; Baptista, S.D.; Campoli, P.V.; Choe, S.I.; Kravtsov, I.; Vinogradov, E.; Creuzenet, C.; Liu, H.; Berghuis, A.M.; Latge, J.P.; Filler, S.G.; Fontaine, T.; Sheppard, D.C.
Overlapping and distinct roles of Aspergillus fumigatus UDP-glucose 4-epimerases in galactose metabolism and the synthesis of galactose-containing cell wall polysaccharides (2014), J. Biol. Chem., 289, 1243-1256.

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Cloned(Commentary)

Cloned (Comment)	Organism
genes uge3 and uge5, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21(DE3), expression as fluorescence labeled proteins uge3-gfp and uge5-rfp in Aspergillus. fumigatus strain Af293	Aspergillus fumigatus

Protein Variants

Protein Variants	Comment	Organism
additional information	generation of single disruption mutants and DELTAuge3 DELTAuge5 double mutant, phenotypes, overview	Aspergillus fumigatus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cell wall	-	Aspergillus fumigatus	5618	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
UDP-alpha-D-glucose	Aspergillus fumigatus	-	UDP-alpha-D-galactose	-	?
UDP-alpha-D-glucose	Aspergillus fumigatus Af293	-	UDP-alpha-D-galactose	-	?

Organism

Organism	UniProt	Comment	Textmining
Aspergillus fumigatus	-	genes uge3 and uge5 encoding isozymes Uge3 and Uge5	-
Aspergillus fumigatus Af293	-	genes uge3 and uge5 encoding isozymes Uge3 and Uge5	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His6-tagged isozymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration	Aspergillus fumigatus

Source Tissue

Source Tissue	Comment	Organism	Textmining
conidium	-	Aspergillus fumigatus	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
UDP-alpha-D-glucose	-	Aspergillus fumigatus	UDP-alpha-D-galactose	-	?
UDP-alpha-D-glucose	-	Aspergillus fumigatus Af293	UDP-alpha-D-galactose	-	?

Subunits

Subunits	Comment	Organism
More	homology structural modeling, overview	Aspergillus fumigatus

Synonyms

Synonyms	Comment	Organism
UGE3	-	Aspergillus fumigatus
UGE5	-	Aspergillus fumigatus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Aspergillus fumigatus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	assay at	Aspergillus fumigatus

General Information

General Information	Comment	Organism
malfunction	In the absence of Uge5, Uge3 activity is sufficient for growth on galactose and the synthesis of galactosaminogalactan containing lower levels of galactose but not the synthesis of galactofuranose. A double deletion of uge5 and uge3 blocked growth on galactose and synthesis of both galactofuranose and galactosaminogalactan, phenotypes, overview	Aspergillus fumigatus
metabolism	overlapping and distinct roles of UDP-glucose 4-epimerases in galactose metabolism and the synthesis of galactose-containing cell wall polysaccharides, galactosaminogalactan synthesis requires the UDP-glucose 4-epimerases, Uge5 and Uge3, whereas galactomannan synthesis requires Uge5 alone, overview	Aspergillus fumigatus
additional information	homology structural modeling, overview	Aspergillus fumigatus
physiological function	Uge5 is the dominant UDP-glucose 4-epimerase in Aspergillus fumigatus and is essential for normal growth in galactose-based medium. Uge5 is required for synthesis of the galactofuranose component of galactomannan and contributes galactose to the synthesis of galactosaminogalactan. Uge3 can mediate production of both UDP-galactose and UDP-N-acetylgalactosamine, cf. EC 5.1.3.7, and is required for the production of galactosaminogalactan but not galactomannan	Aspergillus fumigatus

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Release 2023.1 (January 2023)