Literature summary for 5.1.3.2 extracted from

Schulz, J.M.; Watson, A.L.; Sanders, R.; Ross, K.L.; Thoden, J.B.; Holden, H.M.; Fridovich-Keil, J.L.
Determinants of function and substrate specificity in human UDP-galactose 4'-epimerase (2004), J. Biol. Chem., 279, 32796-32803.

Search for more literature for 5.1.3.2

Cloned(Commentary)

Cloned (Comment)	Organism
mutant enzymes S132A, Y157F, S132A/Y157F and C307Y are expressed in a null-background strain of Saccharomyces cerevisiae. S132a/Y157F and C307Y are also overexpressed in Pichia pastoris	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
C307Y	normal activity with respect to UDP-galactose, complete loss of activity with respect to UDP-N-acetylgalactosamine	Homo sapiens
S132A	complete loss of activity with respect to interconversion of UDP-glucose and UDP-galactose and of UDP-GalNAc and UDP-GlcNAc	Homo sapiens
S132A/Y157F	complete loss of activity with respect to interconversion of UDP-glucose and UDP-galactose and of UDP-GalNAc and UDP-GlcNAc	Homo sapiens
Y157F	complete loss of activity with respect to interconversion of UDP-glucose and UDP-galactose and of UDP-GalNAc and UDP-GlcNAc	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
UDP-galactose	Homo sapiens	final step of the Leloir pathway	UDP-glucose	-	r

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant wild-type and mutant enzymes S132A, Y157F, S132A/Y157F and C307Y	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
UDP-galactose	-	Homo sapiens	UDP-glucose	-	r
UDP-galactose	final step of the Leloir pathway	Homo sapiens	UDP-glucose	-	r
UDP-GalNAc	-	Homo sapiens	UDP-GlcNAc	-	r

Synonyms

Synonyms	Comment	Organism
UDP-galactose 4'-epimerase	-	Homo sapiens

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Release 2023.1 (January 2023)