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Literature summary for 5.1.3.18 extracted from

  • Wolucka, B.A.; Van Montagu, M.
    GDP-mannose 3',5'-epimerase forms GDP-L-gulose, a putative intermediate for the de novo biosynthesis of vitamin C in plants (2003), J. Biol. Chem., 278, 47483-47490.
    View publication on PubMed
Search for more literature for 5.1.3.18

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
GDP potent competitive inhibitor Arabidopsis thaliana
GDP-D-glucose potent competitive inhibitor Arabidopsis thaliana
GDP-L-fucose complex type of inhibition, IC50: 0.07 mM Arabidopsis thaliana
L-ascorbic acid 1 mM, 15% inhibition Arabidopsis thaliana
L-galactono-1,4-lactone 1 mM, 14% inhibition Arabidopsis thaliana
NADH
-
 Arabidopsis thaliana
NADPH
-
 Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0045
-
 GDP-D-mannose native enzyme Arabidopsis thaliana
0.018
-
 GDP-D-mannose recombinant enzyme with N-terminal His-tag Arabidopsis thaliana
0.031
-
 GDP-D-mannose recombinant enzyme with N-terminal glutathione-S-transferase tag Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GDP-D-mannose Arabidopsis thaliana the enzyme catalyzes a reversible epimerization that precedes the committed step in the biosynthesis of vitamin C, resulting in the hydrolysis of the highly energetic glycosyl-diphosphoryl linkage. The plant enzyme undergoes a complex regulation and could control the carbonflux into the vitamin C pathway in response to the redox state of thecell, stress conditions, and GDP-sugar demand for the cell wall glycoprotein biosynthesis GDP-L-galactose
-
 r

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GDP-D-mannose the reaction mixture contains at equilibrium GDP-D-mannose, GDP-L-galactose and GDP-L-gulose in a ratio of 1:0.4:0.2 Arabidopsis thaliana GDP-L-galactose
-
 r
GDP-D-mannose the enzyme catalyzes a reversible epimerization that precedes the committed step in the biosynthesis of vitamin C, resulting in the hydrolysis of the highly energetic glycosyl-diphosphoryl linkage. The plant enzyme undergoes a complex regulation and could control the carbonflux into the vitamin C pathway in response to the redox state of thecell, stress conditions, and GDP-sugar demand for the cell wall glycoprotein biosynthesis Arabidopsis thaliana GDP-L-galactose
-
 r

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.007
-
 GDP-D-mannose recombinant enzyme with N-terminal His-tag Arabidopsis thaliana
0.01
-
 GDP-D-mannose recombinant enzyme with N-terminal glutathione-S-transferase tag Arabidopsis thaliana
0.041
-
 GDP-D-mannose native enzyme Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
NAD+ stimulates Arabidopsis thaliana
NADP+ stimulates Arabidopsis thaliana

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.07
-
 complex type of inhibition, IC50: 0.07 mM Arabidopsis thaliana GDP-L-fucose