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Literature summary for 5.1.3.17 extracted from
- A novel bacterial enzyme with D-glucuronyl C5-epimerase activity (2013), J. Biol. Chem., 288, 24332-24339.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene RED65_08024, design and synthesis of a synthetic based on hypothetical protein RED65_08024, codon optimized for the codon usage bias of Escherichia coli, expression of the recombinant His-tagged enzyme in Escherichia coli strain Rossetta(DE3)/pWUR537 | Bermanella marisrubri |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 51914 | - |
x * 51914, sequence calculation, x * 52000, recombinant enzyme, SDS-PAGE | Bermanella marisrubri |
| 52000 | - |
x * 51914, sequence calculation, x * 52000, recombinant enzyme, SDS-PAGE | Bermanella marisrubri |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Heparosan-N-sulfate D-glucuronate | Bermanella marisrubri | - |
Heparosan-N-sulfate L-iduronate | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bermanella marisrubri | Q1N097 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli strain Rossetta(DE3)/pWUR537 by cobalt affinity chromatography | Bermanella marisrubri |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Heparosan-N-sulfate D-glucuronate | - |
Bermanella marisrubri | Heparosan-N-sulfate L-iduronate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer or tetramer | x * 51914, sequence calculation, x * 52000, recombinant enzyme, SDS-PAGE | Bermanella marisrubri |
| More | RED-C5-epimerase is present in a dimeric/tetrameric form, in approximately a 2:1 ratio, and appears to be in a dynamic equilibrium | Bermanella marisrubri |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| D-glucuronyl C5-epimerase | - |
Bermanella marisrubri |
| RED-C5-epimerase | - |
Bermanella marisrubri |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Bermanella marisrubri |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
purified recombinant His-tagged enzyme, inactivation | Bermanella marisrubri |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | the enzyme is probably involved in bacterial capsular polysaccharide biosynthesis, it shows catalytic properties similar to murine heparan sulfate D-glucuronyl C5-epimerase. The biosynthesis of these complex polysaccharides involves complicated reactions that turn the simple glycosaminoglycan backbone into highly heterogeneous structures. One of the modification reactions is the epimerization of D-glucuronic acid to its C5-epimer L-iduronic acid, which is essential for the function of heparan sulfate | Bermanella marisrubri |
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