Any feedback?
Literature summary for 5.1.3.13 extracted from
- Identification of a dTDP-rhamnose biosynthetic pathway that oscillates with the molting cycle in Caenorhabditis elegans (2016), Biochem. J., 473, 1507-1521 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Caenorhabditis elegans |
| gene rml-3, sequence comparisons and phyylogenetic analysis of rml genes, recombinant expression in Escherichia coli strain BL21(DE3), coexpression with genes rml-1, rml-2, and rml-4 | Caenorhabditis elegans |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | Rml enzymes knockouts by expression of iRNA, phenotypes, overview. RNAi against rml-3 does not affect embryonic or larval development | Caenorhabditis elegans |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dTDP-4-dehydro-6-deoxy-alpha-D-glucose | Caenorhabditis elegans | - |
dTDP-4-dehydro-beta-L-rhamnose | - |
? |  |
| dTDP-4-dehydro-6-deoxy-alpha-D-glucose | Caenorhabditis elegans | - |
dTDP-4-dehydro-beta-L-rhamnose | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Caenorhabditis elegans | Q17993 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Caenorhabditis elegans |
| recombinant enzyme from Escherichia coli strain BL21(DE3) | Caenorhabditis elegans |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| embryo | - |
Caenorhabditis elegans | - |
| hypodermis | - |
Caenorhabditis elegans | - |
| larva | - |
Caenorhabditis elegans | - |
| additional information | transcriptional reporters for the rhamnose biosynthetic genes are expressed highly in the embryo, in the hypodermis during molting cycles and in the hypodermal seam cells specifically before the molt to the stress-resistant dauer larval stage | Caenorhabditis elegans | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dTDP-4-dehydro-6-deoxy-alpha-D-glucose | - |
Caenorhabditis elegans | dTDP-4-dehydro-beta-L-rhamnose | - |
? | |
| dTDP-4-dehydro-6-deoxy-alpha-D-glucose | - |
Caenorhabditis elegans | dTDP-4-dehydro-beta-L-rhamnose | - |
r | |
| additional information | mass spectrometric analysis of reaction products in the assay of coupled enzymes, RML-1, RML-2, RML-3, and RML-4. Reductase activity toward dTDP-4-keto-6-deoxyglucose is observed, but only when both RML-4-RML-5 and RML-3 are added to the reaction mixture in the presence ofNAD(P)H, RML-3 may catalyze the 3,5-epimerization reaction | Caenorhabditis elegans | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 3,5-epimerase | - |
Caenorhabditis elegans |
| C14F11.6 | - |
Caenorhabditis elegans |
| dTDP-4-dehydrorhamnose 3,5-epimerase | - |
Caenorhabditis elegans |
| rml-3 | - |
Caenorhabditis elegans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Caenorhabditis elegans |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | the expression of the rhamnose biosynthetic genes by RNAi results in significant reductions in dTDP-rhamnose, but has no effect on the biosynthesis of a closely related sugar, ascarylose, found in the ascaroside pheromones. RNAi against rml-3 does not affect embryonic or larval development | Caenorhabditis elegans |
| metabolism | biosynthesis of dTDP-L-rhamnose: RML-1 activates glucose 1-phosphate in the presence of either dTTP or UTP to yield dTDP-glucose or UDP-glucose, respectively. RML-2 is a dTDP-glucose 4,6-dehydratase, converting dTDP-glucose into dTDP-4-keto-6-deoxyglucose. And coincubation of dTDP-4-keto-6-deoxyglucose with RML-3 (3,5-epimerase) and RML-4 (4-keto-reductase) produces dTDP-rhamnose. A co-regulated protein, RML-5, forms a complex with RML-4. Rhamnose biosynthesis may play an important role in hypodermal development or the production of the cuticle or surface coat during molting | Caenorhabditis elegans |
| metabolism | the enzyme is involved in the dTDP-rhamnose biosynthetic pathway | Caenorhabditis elegans |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
