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Literature summary for 5.1.3.11 extracted from
- Characterization of an epilactose-producing cellobiose 2-epimerase from Thermoanaerobacterium saccharolyticum (2015), J. Mol. Catal. B, 116, 39-44 .
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene Tsac_2329, phylogenetic tree, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Thermoanaerobacterium saccharolyticum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | 35% inhibition at 1 mM | Thermoanaerobacterium saccharolyticum |  |
| Cu2+ | 20% inhibition at 1 mM | Thermoanaerobacterium saccharolyticum | |
| additional information | no inhibition by EDTA | Thermoanaerobacterium saccharolyticum | |
| Zn2+ | 20% inhibition at 1 mM | Thermoanaerobacterium saccharolyticum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 124.7 | - |
lactose | recombinant enzyme, pH 7.0, 60°C | Thermoanaerobacterium saccharolyticum | |
| 241.1 | - |
cellobiose | recombinant enzyme, pH 7.0, 60°C | Thermoanaerobacterium saccharolyticum | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | the enzyme is metal independent | Thermoanaerobacterium saccharolyticum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| lactose | Thermoanaerobacterium saccharolyticum | - |
epilactose | - |
r | |
| lactose | Thermoanaerobacterium saccharolyticum JW/SL-YS485 / DSM 8691 | - |
epilactose | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermoanaerobacterium saccharolyticum | I3VXT6 | - |
- |
| Thermoanaerobacterium saccharolyticum JW/SL-YS485 / DSM 8691 | I3VXT6 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Thermoanaerobacterium saccharolyticum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 13.5 | - |
substrate lactose, recombinant enzyme, pH 7.0, 60°C | Thermoanaerobacterium saccharolyticum |
| 15.48 | - |
substrate cellobiose, recombinant enzyme, pH 7.0, 60°C | Thermoanaerobacterium saccharolyticum |
| 29.8 | - |
substrate mannobiose, recombinant enzyme, pH 7.0, 60°C | Thermoanaerobacterium saccharolyticum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-O-beta-D-glucosyl-D-mannose | - |
Thermoanaerobacterium saccharolyticum | cellobiose | - |
r | |
| beta-1,4-mannobiose | best substrate | Thermoanaerobacterium saccharolyticum | 4-O-beta-D-mannopyranosyl-D-glucose | - |
r | |
| beta-1,4-mannobiose | best substrate | Thermoanaerobacterium saccharolyticum JW/SL-YS485 / DSM 8691 | 4-O-beta-D-mannopyranosyl-D-glucose | - |
r | |
| cellobiose | - |
Thermoanaerobacterium saccharolyticum | 4-O-beta-D-glucopyranosyl-D-mannose | - |
r | |
| cellobiose | - |
Thermoanaerobacterium saccharolyticum JW/SL-YS485 / DSM 8691 | 4-O-beta-D-glucopyranosyl-D-mannose | - |
r | |
| lactose | - |
Thermoanaerobacterium saccharolyticum | epilactose | - |
r | |
| lactose | at pH 7.0 and 60°C, 50 mM epilactose is produced from 200 mM lactose by a 600 nM of enzyme concentration after reaction for 4 h | Thermoanaerobacterium saccharolyticum | epilactose | - |
r | |
| lactose | - |
Thermoanaerobacterium saccharolyticum JW/SL-YS485 / DSM 8691 | epilactose | - |
r | |
| lactose | at pH 7.0 and 60°C, 50 mM epilactose is produced from 200 mM lactose by a 600 nM of enzyme concentration after reaction for 4 h | Thermoanaerobacterium saccharolyticum JW/SL-YS485 / DSM 8691 | epilactose | - |
r | |
| additional information | no activity with N-acyl-D-glucosamine and D-glucose | Thermoanaerobacterium saccharolyticum | ? | - |
? | |
| additional information | no activity with N-acyl-D-glucosamine and D-glucose | Thermoanaerobacterium saccharolyticum JW/SL-YS485 / DSM 8691 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 46500, recombinant enzyme, SDS-PAGE | Thermoanaerobacterium saccharolyticum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| epilactose-producing cellobiose 2-epimerase | - |
Thermoanaerobacterium saccharolyticum |
| Thsa-CE | - |
Thermoanaerobacterium saccharolyticum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Thermoanaerobacterium saccharolyticum |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 30.9 | - |
lactose | recombinant enzyme, pH 7.0, 60°C | Thermoanaerobacterium saccharolyticum | |
| 100.4 | - |
cellobiose | recombinant enzyme, pH 7.0, 60°C | Thermoanaerobacterium saccharolyticum | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Thermoanaerobacterium saccharolyticum |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.248 | - |
lactose | recombinant enzyme, pH 7.0, 60°C | Thermoanaerobacterium saccharolyticum | |
| 0.417 | - |
cellobiose | recombinant enzyme, pH 7.0, 60°C | Thermoanaerobacterium saccharolyticum | |
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