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Literature summary for 5.1.3.11 extracted from
- Epilactose production by 2 cellobiose 2-epimerases in natural milk (2014), J. Dairy Sci., 97, 155-161 .
Application
| Application | Comment | Organism |
|---|---|---|
| food industry | the enzyme may introduce an added value for particular dairy products by in situ production of the prebiotic sugar epilactose | Flavobacterium johnsoniae |
| food industry | the enzyme may introduce an added value for particular dairy products by in situ production of the prebiotic sugar epilactose | Pedobacter heparinus |
| nutrition | the enzyme may introduce an added value for particular dairy products by in situ production of the prebiotic sugar epilactose | Flavobacterium johnsoniae |
| nutrition | the enzyme may introduce an added value for particular dairy products by in situ production of the prebiotic sugar epilactose | Pedobacter heparinus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA and amino acid sequence determination and analysis, recombinant expression in Escherichia coli | Flavobacterium johnsoniae |
| DNA and amino acid sequence determination and analysis, recombinant expression in Escherichia coli | Pedobacter heparinus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | enzyme production evaluation for large-sclae production, parameters, overview | Flavobacterium johnsoniae |
| additional information | enzyme production evaluation for large-sclae production, parameters, overview | Pedobacter heparinus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| lactose | Flavobacterium johnsoniae | - |
epilactose | - |
r |  |
| lactose | Pedobacter heparinus | - |
epilactose | - |
r | |
| lactose | Pedobacter heparinus ATCC 13125 / DSM 2366 / CIP 104194 / JCM 7457 / NBRC 12017 / NCIMB 9290 / NRRL B-14731 / HIM 762-3 | - |
epilactose | - |
r | |
| lactose | Flavobacterium johnsoniae ATCC 17061 / DSM 2064 / UW101 | - |
epilactose | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Flavobacterium johnsoniae | A5FA14 | - |
- |
| Flavobacterium johnsoniae ATCC 17061 / DSM 2064 / UW101 | A5FA14 | - |
- |
| Pedobacter heparinus | C6XVU9 | - |
- |
| Pedobacter heparinus ATCC 13125 / DSM 2366 / CIP 104194 / JCM 7457 / NBRC 12017 / NCIMB 9290 / NRRL B-14731 / HIM 762-3 | C6XVU9 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme from Escherichia coli | Flavobacterium johnsoniae |
| recombinant enzyme from Escherichia coli | Pedobacter heparinus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| lactose | - |
Flavobacterium johnsoniae | epilactose | - |
r | |
| lactose | - |
Pedobacter heparinus | epilactose | - |
r | |
| lactose | - |
Pedobacter heparinus ATCC 13125 / DSM 2366 / CIP 104194 / JCM 7457 / NBRC 12017 / NCIMB 9290 / NRRL B-14731 / HIM 762-3 | epilactose | - |
r | |
| lactose | - |
Flavobacterium johnsoniae ATCC 17061 / DSM 2064 / UW101 | epilactose | - |
r | |
| additional information | the enzyme is capable of converting lactose to prebiotic epilactose not only in buffer but also in a complex milk system. No side products are detected other than epilactose | Flavobacterium johnsoniae | ? | - |
? | |
| additional information | the enzyme is capable of converting lactose to prebiotic epilactose not only in buffer but also in a complex milk system. No side products are detected other than epilactose | Pedobacter heparinus | ? | - |
? | |
| additional information | the enzyme is capable of converting lactose to prebiotic epilactose not only in buffer but also in a complex milk system. No side products are detected other than epilactose | Pedobacter heparinus ATCC 13125 / DSM 2366 / CIP 104194 / JCM 7457 / NBRC 12017 / NCIMB 9290 / NRRL B-14731 / HIM 762-3 | ? | - |
? | |
| additional information | the enzyme is capable of converting lactose to prebiotic epilactose not only in buffer but also in a complex milk system. No side products are detected other than epilactose | Flavobacterium johnsoniae ATCC 17061 / DSM 2064 / UW101 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| cellobiose 2-epimerase | - |
Flavobacterium johnsoniae |
| cellobiose 2-epimerase | - |
Pedobacter heparinus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Flavobacterium johnsoniae |
| 8 | - |
assay at | Pedobacter heparinus |
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