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Literature summary for 5.1.3.11 extracted from
- Hidden reaction mesophilic cellobiose 2-epimerases produce lactulose (2017), J. Agric. Food Chem., 65, 2530-2539 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene B15CE, sequence comparisons and phylogenetic analysis, recombinant expression of codon-optimized His-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain XL-1 Blue, the recombinant strain produces lactulose | Butyrivibrio sp. AE2015 |
| gene bfce, sequence comparisons and phylogenetic analysis, recombinant expression of codon-optimized His-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain XL-1 Blue, the recombinant strain produces lactulose | Bacteroides fragilis |
| gene BN699_02313, sequence comparisons and phylogenetic analysis, recombinant expression of codon-optimized His-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain XL-1 Blue, the recombinant strain produces lactulose | Firmicutes bacterium |
| gene ce, sequence comparisons and phylogenetic analysis, recombinant expression of codon-optimized His-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain XL-1 Blue, the recombinant strain produces lactulose | Rhodothermus marinus |
| gene ce-ne1, sequence comparisons and phylogenetic analysis, recombinant expression of codon-optimized His-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain XL-1 Blue, the recombinant strain produces lactulose | Ruminococcus albus |
| gene Dfer_1975, sequence comparisons and phylogenetic analysis, recombinant expression of codon-optimized His-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain XL-1 Blue, the recombinant strain produces lactulose | Dyadobacter fermentans |
| gene Rmar_2439, sequence comparisons and phylogenetic analysis, recombinant expression of codon-optimized His-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain XL-1 Blue, the recombinant strain produces lactulose | Rhodothermus marinus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| lactose | Ruminococcus albus | - |
epilactose | - |
r |  |
| lactose | Bacteroides fragilis | - |
epilactose | - |
r | |
| lactose | Rhodothermus marinus | - |
epilactose | - |
r | |
| lactose | Dyadobacter fermentans | - |
epilactose | - |
r | |
| lactose | Firmicutes bacterium | - |
epilactose | - |
r | |
| lactose | Butyrivibrio sp. AE2015 | - |
epilactose | - |
r | |
| lactose | Ruminococcus albus | - |
lactulose | - |
r | |
| lactose | Bacteroides fragilis | - |
lactulose | - |
r | |
| lactose | Rhodothermus marinus | - |
lactulose | - |
r | |
| lactose | Dyadobacter fermentans | - |
lactulose | - |
r | |
| lactose | Firmicutes bacterium | - |
lactulose | - |
r | |
| lactose | Butyrivibrio sp. AE2015 | - |
lactulose | - |
r | |
| lactose | Rhodothermus marinus ATCC 43812 / DSM 4252 / R-10 | - |
epilactose | - |
r | |
| lactose | Rhodothermus marinus ATCC 43812 / DSM 4252 / R-10 | - |
lactulose | - |
r | |
| lactose | Bacteroides fragilis DSM 2151 | - |
epilactose | - |
r | |
| lactose | Bacteroides fragilis DSM 2151 | - |
lactulose | - |
r | |
| lactose | Ruminococcus albus DSM 20455 | - |
epilactose | - |
r | |
| lactose | Ruminococcus albus DSM 20455 | - |
lactulose | - |
r | |
| lactose | Dyadobacter fermentans ATCC 700827 / DSM 18053 / NS114 | - |
epilactose | - |
r | |
| lactose | Dyadobacter fermentans ATCC 700827 / DSM 18053 / NS114 | - |
lactulose | - |
r | |
| lactose | Firmicutes bacterium CAG:534 | - |
epilactose | - |
r | |
| lactose | Firmicutes bacterium CAG:534 | - |
lactulose | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacteroides fragilis | Q5LH66 | - |
- |
| Bacteroides fragilis DSM 2151 | Q5LH66 | - |
- |
| Butyrivibrio sp. AE2015 | - |
- |
- |
| Dyadobacter fermentans | C6VW66 | - |
- |
| Dyadobacter fermentans ATCC 700827 / DSM 18053 / NS114 | C6VW66 | - |
- |
| Firmicutes bacterium | R7AVK7 | - |
- |
| Firmicutes bacterium CAG:534 | R7AVK7 | - |
- |
| Rhodothermus marinus | D0MF61 | - |
- |
| Rhodothermus marinus | F8WRK9 | - |
- |
| Rhodothermus marinus ATCC 43812 / DSM 4252 / R-10 | D0MF61 | - |
- |
| Ruminococcus albus | P0DKY4 | - |
- |
| Ruminococcus albus DSM 20455 | P0DKY4 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and desalting gel filtration | Ruminococcus albus |
| recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and desalting gel filtration | Bacteroides fragilis |
| recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and desalting gel filtration | Rhodothermus marinus |
| recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and desalting gel filtration | Dyadobacter fermentans |
| recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and desalting gel filtration | Firmicutes bacterium |
| recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and desalting gel filtration | Butyrivibrio sp. AE2015 |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 25.85 | - |
recombinant enzyme activity in vivo, substrate lactose, pH 7.0, 10°C | Butyrivibrio sp. AE2015 |
| 38.81 | - |
recombinant enzyme activity in vivo, substrate lactose, pH 7.0, 10°C | Ruminococcus albus |
| 46.91 | - |
recombinant enzyme activity in vivo, substrate lactose, pH 7.0, 10°C | Dyadobacter fermentans |
| 58.91 | - |
recombinant enzyme activity in vivo, substrate lactose, pH 7.0, 10°C | Firmicutes bacterium |
| 76.78 | - |
recombinant enzyme activity in vivo, substrate lactose, pH 7.0, 10°C | Bacteroides fragilis |
| 311.34 | - |
recombinant enzyme activity in vivo, substrate lactose, pH 7.0, 10°C | Rhodothermus marinus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| lactose | - |
Ruminococcus albus | epilactose | - |
r | |
| lactose | - |
Bacteroides fragilis | epilactose | - |
r | |
| lactose | - |
Rhodothermus marinus | epilactose | - |
r | |
| lactose | - |
Dyadobacter fermentans | epilactose | - |
r | |
| lactose | - |
Firmicutes bacterium | epilactose | - |
r | |
| lactose | - |
Butyrivibrio sp. AE2015 | epilactose | - |
r | |
| lactose | - |
Rhodothermus marinus ATCC 43812 / DSM 4252 / R-10 | epilactose | - |
r | |
| lactose | - |
Bacteroides fragilis DSM 2151 | epilactose | - |
r | |
| lactose | - |
Ruminococcus albus DSM 20455 | epilactose | - |
r | |
| lactose | - |
Dyadobacter fermentans ATCC 700827 / DSM 18053 / NS114 | epilactose | - |
r | |
| lactose | - |
Firmicutes bacterium CAG:534 | epilactose | - |
r | |
| lactose | - |
Ruminococcus albus | lactulose | - |
r | |
| lactose | - |
Bacteroides fragilis | lactulose | - |
r | |
| lactose | - |
Rhodothermus marinus | lactulose | - |
r | |
| lactose | - |
Dyadobacter fermentans | lactulose | - |
r | |
| lactose | - |
Firmicutes bacterium | lactulose | - |
r | |
| lactose | - |
Butyrivibrio sp. AE2015 | lactulose | - |
r | |
| lactose | - |
Rhodothermus marinus ATCC 43812 / DSM 4252 / R-10 | lactulose | - |
r | |
| lactose | - |
Bacteroides fragilis DSM 2151 | lactulose | - |
r | |
| lactose | - |
Ruminococcus albus DSM 20455 | lactulose | - |
r | |
| lactose | - |
Dyadobacter fermentans ATCC 700827 / DSM 18053 / NS114 | lactulose | - |
r | |
| lactose | - |
Firmicutes bacterium CAG:534 | lactulose | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 42000, about, recombinant His-tagged enzyme, SDS-PAGE | Ruminococcus albus |
| ? | x * 42000, about, recombinant His-tagged enzyme, SDS-PAGE | Bacteroides fragilis |
| ? | x * 42000, about, recombinant His-tagged enzyme, SDS-PAGE | Rhodothermus marinus |
| ? | x * 42000, about, recombinant His-tagged enzyme, SDS-PAGE | Dyadobacter fermentans |
| ? | x * 42000, about, recombinant His-tagged enzyme, SDS-PAGE | Firmicutes bacterium |
| ? | x * 42000, about, recombinant His-tagged enzyme, SDS-PAGE, x * 47400, about, sequence calculation | Butyrivibrio sp. AE2015 |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| B15CE | - |
Butyrivibrio sp. AE2015 |
| BfCE | - |
Bacteroides fragilis |
| BN699_02313 | - |
Firmicutes bacterium |
| CE-NE1 | - |
Ruminococcus albus |
| cellobiose 2-epimerase | - |
Ruminococcus albus |
| cellobiose 2-epimerase | - |
Bacteroides fragilis |
| cellobiose 2-epimerase | - |
Rhodothermus marinus |
| cellobiose 2-epimerase | - |
Dyadobacter fermentans |
| cellobiose 2-epimerase | - |
Firmicutes bacterium |
| cellobiose 2-epimerase | - |
Butyrivibrio sp. AE2015 |
| DfCE | - |
Dyadobacter fermentans |
| Dfer_1975 | - |
Dyadobacter fermentans |
| FbCE | - |
Firmicutes bacterium |
| RACE | - |
Ruminococcus albus |
| Rmar_2439 | - |
Rhodothermus marinus |
| RmCE | - |
Rhodothermus marinus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Ruminococcus albus |
| 45 | - |
assay at | Bacteroides fragilis |
| 45 | - |
assay at | Firmicutes bacterium |
| 50 | - |
assay at | Dyadobacter fermentans |
| 60 | - |
assay at | Butyrivibrio sp. AE2015 |
| 80 | - |
assay at | Rhodothermus marinus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.3 | - |
assay at | Rhodothermus marinus |
| 7 | - |
assay at | Butyrivibrio sp. AE2015 |
| 7.5 | - |
assay at | Ruminococcus albus |
| 7.5 | - |
assay at | Bacteroides fragilis |
| 7.5 | - |
assay at | Firmicutes bacterium |
| 7.7 | - |
assay at | Dyadobacter fermentans |
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