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Literature summary for 5.1.3.11 extracted from
- Lactulose production from lactose by recombinant cellobiose 2-epimerase in permeabilised Escherichia coli cells (2015), Int. J. Food Sci. Technol., 50, 1625-1631 .
No PubMed abstract available
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | most efficient enzyme for the enzymatic synthesis of lactulose. Ethanol-permeabilised Escherichia coli cells containing the enzyme are used as biocatalysts for lactulose production. The reaction conditions for maximum lactulose production are optimised to be 600 g/l lactose, pH 7.5, 80°C and 12.5 U/ml of whole-cell biocatalyst. After incubated at Na2HPO4-NaH2PO4 buffer for 2 h, approximately 390.59 g/l lactulose is obtained with a conversion yield of 65.1%. The lowest production and conversion yield of epilactose are also found in Na2HPO4-NaH2PO4 buffer with a final concentration of 11.7 g/l and a conversion yield less than 2%. The results represent a promising technology to attain high production and conversion yield of lactulose with a high purity on industrial scale | Caldicellulosiruptor saccharolyticus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli BL21 (DE3) | Caldicellulosiruptor saccharolyticus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | lactulose production from lactose by recombinant cellobiose 2-epimerase in permeabilised Escherichia coli ethanol-permeabilised cells. The reaction conditions for maximum lactulose production are optimised to be 600 g/l lactose, pH 7.5, 80°C and 12.5 U ml of whole-cell biocatalyst. After incubated at Na2HPO4-NaH2PO4 buffer for 2 h, approximately 390.59 g/l lactulose is obtained with a conversion yield of 65.1%. Method optimization, detailed overview. A Na2HPO4-NaH2PO4 buffer system results in the highest lactulose conversion yield and the lowest epilactose production | Caldicellulosiruptor saccharolyticus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| lactose | Caldicellulosiruptor saccharolyticus | - |
lactulose | - |
r |  |
| lactose | Caldicellulosiruptor saccharolyticus ATCC 43494 / DSM 8903 / Tp8T 6331 | - |
lactulose | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Caldicellulosiruptor saccharolyticus | A4XGA6 | - |
- |
| Caldicellulosiruptor saccharolyticus ATCC 43494 / DSM 8903 / Tp8T 6331 | A4XGA6 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| lactose | - |
Caldicellulosiruptor saccharolyticus | epilactose | - |
? | |
| lactose | - |
Caldicellulosiruptor saccharolyticus ATCC 43494 / DSM 8903 / Tp8T 6331 | epilactose | - |
? | |
| lactose | - |
Caldicellulosiruptor saccharolyticus | lactulose | - |
? | |
| lactose | - |
Caldicellulosiruptor saccharolyticus | lactulose | - |
r | |
| lactose | - |
Caldicellulosiruptor saccharolyticus ATCC 43494 / DSM 8903 / Tp8T 6331 | lactulose | - |
? | |
| lactose | - |
Caldicellulosiruptor saccharolyticus ATCC 43494 / DSM 8903 / Tp8T 6331 | lactulose | - |
r | |
| additional information | enzyme CsCE not only has the ability to convert lactose into lactulose, but can also epimerise lactose into epilactose. A Na2HPO4-NaH2PO4 buffer system results in the highest lactulose conversion yield and the lowest epilactose production | Caldicellulosiruptor saccharolyticus | ? | - |
? | |
| additional information | enzyme CsCE not only has the ability to convert lactose into lactulose, but can also epimerise lactose into epilactose. A Na2HPO4-NaH2PO4 buffer system results in the highest lactulose conversion yield and the lowest epilactose production | Caldicellulosiruptor saccharolyticus ATCC 43494 / DSM 8903 / Tp8T 6331 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| cellobiose 2-epimerase | - |
Caldicellulosiruptor saccharolyticus |
| Csac_0294 | - |
Caldicellulosiruptor saccharolyticus |
| CsCE | - |
Caldicellulosiruptor saccharolyticus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
- |
Caldicellulosiruptor saccharolyticus |
| 80 | - |
assay at | Caldicellulosiruptor saccharolyticus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 10 | 90 | activity range, above 10% of maximal activity within this range | Caldicellulosiruptor saccharolyticus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Caldicellulosiruptor saccharolyticus |
| 7.5 | - |
assay at | Caldicellulosiruptor saccharolyticus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8.5 | over 60% activity within this range | Caldicellulosiruptor saccharolyticus |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | Caldicellulosiruptor saccharolyticus cellobiose 2-epimerase (CsCE) catalyses the single substrate lactose into lactulose and is a highly efficient enzyme for the enzymatic synthesis of lactulose | Caldicellulosiruptor saccharolyticus |
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