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Literature summary for 5.1.3.11 extracted from
- Functions, structures, and applications of cellobiose 2-epimerase and glycoside hydrolase family 130 mannoside phosphorylases (2016), Biosci. Biotechnol. Biochem., 80, 1294-1305 .
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | application of the enzyme for production of functional oligosaccharides, overview | Ruminococcus albus |
| synthesis | application of the enzyme for production of functional oligosaccharides, overview | [Eubacterium] cellulosolvens |
| synthesis | application of the enzyme for production of functional oligosaccharides, overview | Bacteroides fragilis |
| synthesis | application of the enzyme for production of functional oligosaccharides, overview | Caldicellulosiruptor saccharolyticus |
| synthesis | application of the enzyme for production of functional oligosaccharides, overview | Rhodothermus marinus |
| synthesis | application of the enzyme for production of functional oligosaccharides, overview | Dyadobacter fermentans |
| synthesis | application of the enzyme for production of functional oligosaccharides, overview | Dictyoglomus turgidum |
| synthesis | application of the enzyme for production of functional oligosaccharides, overview | Flavobacterium johnsoniae |
| synthesis | application of the enzyme for production of functional oligosaccharides, overview | Herpetosiphon aurantiacus |
| synthesis | application of the enzyme for production of functional oligosaccharides, overview | Pedobacter heparinus |
| synthesis | application of the enzyme for production of functional oligosaccharides, overview | Saccharophagus degradans |
| synthesis | application of the enzyme for production of functional oligosaccharides, overview | Spirosoma linguale |
| synthesis | application of the enzyme for production of functional oligosaccharides, overview | Spirochaeta thermophila |
| synthesis | application of the enzyme for production of functional oligosaccharides, overview | Teredinibacter turnerae |
| synthesis | application of the enzyme for production of functional oligosaccharides, overview | Cellvibrio vulgaris |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| sequence comparisons and phylogenetic tree, recombinant expression | Ruminococcus albus |
| sequence comparisons and phylogenetic tree, recombinant expression | [Eubacterium] cellulosolvens |
| sequence comparisons and phylogenetic tree, recombinant expression | Bacteroides fragilis |
| sequence comparisons and phylogenetic tree, recombinant expression | Caldicellulosiruptor saccharolyticus |
| sequence comparisons and phylogenetic tree, recombinant expression | Rhodothermus marinus |
| sequence comparisons and phylogenetic tree, recombinant expression | Dyadobacter fermentans |
| sequence comparisons and phylogenetic tree, recombinant expression | Dictyoglomus turgidum |
| sequence comparisons and phylogenetic tree, recombinant expression | Flavobacterium johnsoniae |
| sequence comparisons and phylogenetic tree, recombinant expression | Herpetosiphon aurantiacus |
| sequence comparisons and phylogenetic tree, recombinant expression | Pedobacter heparinus |
| sequence comparisons and phylogenetic tree, recombinant expression | Saccharophagus degradans |
| sequence comparisons and phylogenetic tree, recombinant expression | Spirosoma linguale |
| sequence comparisons and phylogenetic tree, recombinant expression | Spirochaeta thermophila |
| sequence comparisons and phylogenetic tree, recombinant expression | Teredinibacter turnerae |
| sequence comparisons and phylogenetic tree, recombinant expression | Cellvibrio vulgaris |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.5 | - |
4-O-beta-D-mannopyranosyl-beta-D-mannopyranose | pH 7.5, 30°C, recombinant enzyme | Spirochaeta thermophila |  |
| 3.7 | 5 | cellobiose | pH 7.5, 30°C, recombinant enzyme | Bacteroides fragilis | |
| 4.03 | - |
4-O-beta-D-mannopyranosyl-beta-D-mannopyranose | pH 7.5, 30°C, recombinant enzyme | Cellvibrio vulgaris | |
| 4.3 | - |
cellobiose | pH 7.5, 30°C, recombinant enzyme | Spirochaeta thermophila | |
| 5.25 | - |
4-O-beta-D-mannopyranosyl-beta-D-mannopyranose | pH 7.5, 30°C, recombinant enzyme | Rhodothermus marinus | |
| 5.51 | - |
4-O-beta-D-mannopyranosyl-beta-D-mannopyranose | pH 7.5, 30°C, recombinant enzyme | Bacteroides fragilis | |
| 6.56 | - |
lactose | pH 7.5, 30°C, recombinant enzyme | Bacteroides fragilis | |
| 8.74 | - |
4-O-beta-D-mannopyranosyl-beta-D-mannopyranose | pH 7.5, 30°C, recombinant enzyme | Ruminococcus albus | |
| 11.3 | - |
cellobiose | pH 7.5, 30°C, recombinant enzyme | [Eubacterium] cellulosolvens | |
| 13.8 | - |
cellobiose | pH 7.5, 30°C, recombinant enzyme | Ruminococcus albus | |
| 22.6 | - |
cellobiose | pH 7.5, 30°C, recombinant enzyme | Saccharophagus degradans | |
| 24.5 | - |
cellobiose | pH 7.5, 30°C, recombinant enzyme | Cellvibrio vulgaris | |
| 24.5 | - |
lactose | pH 7.5, 30°C, recombinant enzyme | Pedobacter heparinus | |
| 27.2 | - |
cellobiose | pH 7.5, 30°C, recombinant enzyme | Rhodothermus marinus | |
| 28.2 | - |
cellobiose | pH 7.5, 30°C, recombinant enzyme | Herpetosiphon aurantiacus | |
| 28.7 | - |
lactose | pH 7.5, 30°C, recombinant enzyme | Cellvibrio vulgaris | |
| 28.8 | - |
lactose | pH 7.5, 30°C, recombinant enzyme | Rhodothermus marinus | |
| 29.2 | - |
lactose | pH 7.5, 30°C, recombinant enzyme | Saccharophagus degradans | |
| 29.6 | - |
cellobiose | pH 7.5, 30°C, recombinant enzyme | Pedobacter heparinus | |
| 33 | - |
lactose | pH 7.5, 30°C, recombinant enzyme | Ruminococcus albus | |
| 34.9 | - |
lactose | pH 7.5, 30°C, recombinant enzyme | Flavobacterium johnsoniae | |
| 51.9 | - |
lactose | pH 7.5, 30°C, recombinant enzyme | Herpetosiphon aurantiacus | |
| 53.2 | - |
cellobiose | pH 7.5, 30°C, recombinant enzyme | Flavobacterium johnsoniae | |
| 72 | - |
lactose | pH 7.5, 30°C, recombinant enzyme | [Eubacterium] cellulosolvens | |
| 75.8 | - |
cellobiose | pH 7.5, 30°C, recombinant enzyme | Dictyoglomus turgidum | |
| 79.6 | - |
lactose | pH 7.5, 30°C, recombinant enzyme | Dictyoglomus turgidum | |
| 95.7 | - |
lactose | pH 7.5, 30°C, recombinant enzyme | Dyadobacter fermentans | |
| 104 | - |
cellobiose | pH 7.5, 30°C, recombinant enzyme | Spirosoma linguale | |
| 179 | - |
cellobiose | pH 7.5, 30°C, recombinant enzyme | Dyadobacter fermentans | |
| 198 | - |
cellobiose | pH 7.5, 30°C, recombinant enzyme | Teredinibacter turnerae | |
| 206 | - |
lactose | pH 7.5, 30°C, recombinant enzyme | Spirosoma linguale | |
| 238 | - |
lactose | pH 7.5, 30°C, recombinant enzyme | Teredinibacter turnerae | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cellobiose | Ruminococcus albus | - |
4-O-beta-D-glucopyranosyl-D-mannose | - |
r | |
| cellobiose | [Eubacterium] cellulosolvens | - |
4-O-beta-D-glucopyranosyl-D-mannose | - |
r | |
| cellobiose | Bacteroides fragilis | - |
4-O-beta-D-glucopyranosyl-D-mannose | - |
r | |
| cellobiose | Caldicellulosiruptor saccharolyticus | - |
4-O-beta-D-glucopyranosyl-D-mannose | - |
r | |
| cellobiose | Rhodothermus marinus | - |
4-O-beta-D-glucopyranosyl-D-mannose | - |
r | |
| cellobiose | Dyadobacter fermentans | - |
4-O-beta-D-glucopyranosyl-D-mannose | - |
r | |
| cellobiose | Dictyoglomus turgidum | - |
4-O-beta-D-glucopyranosyl-D-mannose | - |
r | |
| cellobiose | Flavobacterium johnsoniae | - |
4-O-beta-D-glucopyranosyl-D-mannose | - |
r | |
| cellobiose | Herpetosiphon aurantiacus | - |
4-O-beta-D-glucopyranosyl-D-mannose | - |
r | |
| cellobiose | Pedobacter heparinus | - |
4-O-beta-D-glucopyranosyl-D-mannose | - |
r | |
| cellobiose | Saccharophagus degradans | - |
4-O-beta-D-glucopyranosyl-D-mannose | - |
r | |
| cellobiose | Spirosoma linguale | - |
4-O-beta-D-glucopyranosyl-D-mannose | - |
r | |
| cellobiose | Spirochaeta thermophila | - |
4-O-beta-D-glucopyranosyl-D-mannose | - |
r | |
| cellobiose | Teredinibacter turnerae | - |
4-O-beta-D-glucopyranosyl-D-mannose | - |
r | |
| cellobiose | Cellvibrio vulgaris | - |
4-O-beta-D-glucopyranosyl-D-mannose | - |
r | |
| cellobiose | Bacteroides fragilis ATCC 25285 / DSM 2151 / JCM 11019 / NCTC 9343 | - |
4-O-beta-D-glucopyranosyl-D-mannose | - |
r | |
| cellobiose | Spirochaeta thermophila ATCC 49972 / DSM 6192 / RI 19.B1 | - |
4-O-beta-D-glucopyranosyl-D-mannose | - |
r | |
| cellobiose | Caldicellulosiruptor saccharolyticus ATCC 43494 / DSM 8903 / Tp8T 6331 | - |
4-O-beta-D-glucopyranosyl-D-mannose | - |
r | |
| cellobiose | Herpetosiphon aurantiacus ATCC 23779 / DSM 785 | - |
4-O-beta-D-glucopyranosyl-D-mannose | - |
r | |
| cellobiose | Spirosoma linguale ATCC 33905 / DSM 74 / LMG 10896 | - |
4-O-beta-D-glucopyranosyl-D-mannose | - |
r | |
| cellobiose | Pedobacter heparinus ATCC 13125 / DSM 2366 / CIP 104194 / JCM 7457 / NBRC 12017 / NCIMB 9290 / NRRL B-14731 / HIM 762-3 | - |
4-O-beta-D-glucopyranosyl-D-mannose | - |
r | |
| cellobiose | Saccharophagus degradans 2-40 / ATCC 43961 / DSM 17024 | - |
4-O-beta-D-glucopyranosyl-D-mannose | - |
r | |
| cellobiose | Teredinibacter turnerae ATCC 39867 / T7901 | - |
4-O-beta-D-glucopyranosyl-D-mannose | - |
r | |
| cellobiose | Dyadobacter fermentans ATCC 700827 / DSM 18053 / NS114 | - |
4-O-beta-D-glucopyranosyl-D-mannose | - |
r | |
| cellobiose | Flavobacterium johnsoniae ATCC 17061 / DSM 2064 / UW101 | - |
4-O-beta-D-glucopyranosyl-D-mannose | - |
r | |
| cellobiose | Dictyoglomus turgidum Z-1310 / DSM 6724 | - |
4-O-beta-D-glucopyranosyl-D-mannose | - |
r | |
| cellobiose | Spirochaeta thermophila ATCC 700085 / DSM 6578 / Z-1203 | - |
4-O-beta-D-glucopyranosyl-D-mannose | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacteroides fragilis | Q5LH66 | - |
- |
| Bacteroides fragilis ATCC 25285 / DSM 2151 / JCM 11019 / NCTC 9343 | Q5LH66 | - |
- |
| Caldicellulosiruptor saccharolyticus | A4XGA6 | - |
- |
| Caldicellulosiruptor saccharolyticus ATCC 43494 / DSM 8903 / Tp8T 6331 | A4XGA6 | - |
- |
| Cellvibrio vulgaris | - |
- |
- |
| Dictyoglomus turgidum | B8DZK4 | - |
- |
| Dictyoglomus turgidum Z-1310 / DSM 6724 | B8DZK4 | - |
- |
| Dyadobacter fermentans | C6VW66 | - |
- |
| Dyadobacter fermentans ATCC 700827 / DSM 18053 / NS114 | C6VW66 | - |
- |
| Flavobacterium johnsoniae | A5FA14 | - |
- |
| Flavobacterium johnsoniae ATCC 17061 / DSM 2064 / UW101 | A5FA14 | - |
- |
| Herpetosiphon aurantiacus | A9AYF3 | - |
- |
| Herpetosiphon aurantiacus ATCC 23779 / DSM 785 | A9AYF3 | - |
- |
| Pedobacter heparinus | C6XVU9 | - |
- |
| Pedobacter heparinus ATCC 13125 / DSM 2366 / CIP 104194 / JCM 7457 / NBRC 12017 / NCIMB 9290 / NRRL B-14731 / HIM 762-3 | C6XVU9 | - |
- |
| Rhodothermus marinus | F8WRK9 | - |
- |
| Ruminococcus albus | P0DKY4 | - |
- |
| Saccharophagus degradans | Q21NF7 | - |
- |
| Saccharophagus degradans 2-40 / ATCC 43961 / DSM 17024 | Q21NF7 | - |
- |
| Spirochaeta thermophila | E0RU15 | - |
- |
| Spirochaeta thermophila | G0GBL0 | - |
- |
| Spirochaeta thermophila ATCC 49972 / DSM 6192 / RI 19.B1 | E0RU15 | - |
- |
| Spirochaeta thermophila ATCC 700085 / DSM 6578 / Z-1203 | G0GBL0 | - |
- |
| Spirosoma linguale | D2QDA5 | - |
- |
| Spirosoma linguale ATCC 33905 / DSM 74 / LMG 10896 | D2QDA5 | - |
- |
| Teredinibacter turnerae | C5BUF0 | - |
- |
| Teredinibacter turnerae | K7ZLA5 | - |
- |
| Teredinibacter turnerae ATCC 39867 / T7901 | C5BUF0 | - |
- |
| [Eubacterium] cellulosolvens | B3XZI5 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity | Ruminococcus albus |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| cellobiose = 4-O-beta-D-glucopyranosyl-D-mannose | reaction mechansim, overview | Ruminococcus albus | |
| cellobiose = 4-O-beta-D-glucopyranosyl-D-mannose | reaction mechansim, overview | [Eubacterium] cellulosolvens | |
| cellobiose = 4-O-beta-D-glucopyranosyl-D-mannose | reaction mechansim, overview | Bacteroides fragilis | |
| cellobiose = 4-O-beta-D-glucopyranosyl-D-mannose | reaction mechansim, overview | Caldicellulosiruptor saccharolyticus | |
| cellobiose = 4-O-beta-D-glucopyranosyl-D-mannose | reaction mechansim, overview | Rhodothermus marinus | |
| cellobiose = 4-O-beta-D-glucopyranosyl-D-mannose | reaction mechansim, overview | Dyadobacter fermentans | |
| cellobiose = 4-O-beta-D-glucopyranosyl-D-mannose | reaction mechansim, overview | Dictyoglomus turgidum | |
| cellobiose = 4-O-beta-D-glucopyranosyl-D-mannose | reaction mechansim, overview | Flavobacterium johnsoniae | |
| cellobiose = 4-O-beta-D-glucopyranosyl-D-mannose | reaction mechansim, overview | Herpetosiphon aurantiacus | |
| cellobiose = 4-O-beta-D-glucopyranosyl-D-mannose | reaction mechansim, overview | Pedobacter heparinus | |
| cellobiose = 4-O-beta-D-glucopyranosyl-D-mannose | reaction mechansim, overview | Saccharophagus degradans | |
| cellobiose = 4-O-beta-D-glucopyranosyl-D-mannose | reaction mechansim, overview | Spirosoma linguale | |
| cellobiose = 4-O-beta-D-glucopyranosyl-D-mannose | reaction mechansim, overview | Spirochaeta thermophila | |
| cellobiose = 4-O-beta-D-glucopyranosyl-D-mannose | reaction mechansim, overview | Teredinibacter turnerae | |
| cellobiose = 4-O-beta-D-glucopyranosyl-D-mannose | reaction mechansim, overview | Cellvibrio vulgaris |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-O-beta-D-mannopyranosyl-beta-D-mannopyranose | - |
Ruminococcus albus | beta-D-mannopyranosyl-(1->4)-D-glucose | - |
? | |
| 4-O-beta-D-mannopyranosyl-beta-D-mannopyranose | - |
[Eubacterium] cellulosolvens | beta-D-mannopyranosyl-(1->4)-D-glucose | - |
? | |
| 4-O-beta-D-mannopyranosyl-beta-D-mannopyranose | - |
Bacteroides fragilis | beta-D-mannopyranosyl-(1->4)-D-glucose | - |
? | |
| 4-O-beta-D-mannopyranosyl-beta-D-mannopyranose | - |
Caldicellulosiruptor saccharolyticus | beta-D-mannopyranosyl-(1->4)-D-glucose | - |
? | |
| 4-O-beta-D-mannopyranosyl-beta-D-mannopyranose | - |
Rhodothermus marinus | beta-D-mannopyranosyl-(1->4)-D-glucose | - |
? | |
| 4-O-beta-D-mannopyranosyl-beta-D-mannopyranose | - |
Dyadobacter fermentans | beta-D-mannopyranosyl-(1->4)-D-glucose | - |
? | |
| 4-O-beta-D-mannopyranosyl-beta-D-mannopyranose | - |
Dictyoglomus turgidum | beta-D-mannopyranosyl-(1->4)-D-glucose | - |
? | |
| 4-O-beta-D-mannopyranosyl-beta-D-mannopyranose | - |
Flavobacterium johnsoniae | beta-D-mannopyranosyl-(1->4)-D-glucose | - |
? | |
| 4-O-beta-D-mannopyranosyl-beta-D-mannopyranose | - |
Herpetosiphon aurantiacus | beta-D-mannopyranosyl-(1->4)-D-glucose | - |
? | |
| 4-O-beta-D-mannopyranosyl-beta-D-mannopyranose | - |
Pedobacter heparinus | beta-D-mannopyranosyl-(1->4)-D-glucose | - |
? | |
| 4-O-beta-D-mannopyranosyl-beta-D-mannopyranose | - |
Saccharophagus degradans | beta-D-mannopyranosyl-(1->4)-D-glucose | - |
? | |
| 4-O-beta-D-mannopyranosyl-beta-D-mannopyranose | - |
Spirosoma linguale | beta-D-mannopyranosyl-(1->4)-D-glucose | - |
? | |
| 4-O-beta-D-mannopyranosyl-beta-D-mannopyranose | - |
Spirochaeta thermophila | beta-D-mannopyranosyl-(1->4)-D-glucose | - |
? | |
| 4-O-beta-D-mannopyranosyl-beta-D-mannopyranose | - |
Teredinibacter turnerae | beta-D-mannopyranosyl-(1->4)-D-glucose | - |
? | |
| 4-O-beta-D-mannopyranosyl-beta-D-mannopyranose | - |
Cellvibrio vulgaris | beta-D-mannopyranosyl-(1->4)-D-glucose | - |
? | |
| 4-O-beta-D-mannopyranosyl-beta-D-mannopyranose | - |
Bacteroides fragilis ATCC 25285 / DSM 2151 / JCM 11019 / NCTC 9343 | beta-D-mannopyranosyl-(1->4)-D-glucose | - |
? | |
| 4-O-beta-D-mannopyranosyl-beta-D-mannopyranose | - |
Spirochaeta thermophila ATCC 49972 / DSM 6192 / RI 19.B1 | beta-D-mannopyranosyl-(1->4)-D-glucose | - |
? | |
| 4-O-beta-D-mannopyranosyl-beta-D-mannopyranose | - |
Caldicellulosiruptor saccharolyticus ATCC 43494 / DSM 8903 / Tp8T 6331 | beta-D-mannopyranosyl-(1->4)-D-glucose | - |
? | |
| 4-O-beta-D-mannopyranosyl-beta-D-mannopyranose | - |
Herpetosiphon aurantiacus ATCC 23779 / DSM 785 | beta-D-mannopyranosyl-(1->4)-D-glucose | - |
? | |
| 4-O-beta-D-mannopyranosyl-beta-D-mannopyranose | - |
Spirosoma linguale ATCC 33905 / DSM 74 / LMG 10896 | beta-D-mannopyranosyl-(1->4)-D-glucose | - |
? | |
| 4-O-beta-D-mannopyranosyl-beta-D-mannopyranose | - |
Pedobacter heparinus ATCC 13125 / DSM 2366 / CIP 104194 / JCM 7457 / NBRC 12017 / NCIMB 9290 / NRRL B-14731 / HIM 762-3 | beta-D-mannopyranosyl-(1->4)-D-glucose | - |
? | |
| 4-O-beta-D-mannopyranosyl-beta-D-mannopyranose | - |
Saccharophagus degradans 2-40 / ATCC 43961 / DSM 17024 | beta-D-mannopyranosyl-(1->4)-D-glucose | - |
? | |
| 4-O-beta-D-mannopyranosyl-beta-D-mannopyranose | - |
Teredinibacter turnerae ATCC 39867 / T7901 | beta-D-mannopyranosyl-(1->4)-D-glucose | - |
? | |
| 4-O-beta-D-mannopyranosyl-beta-D-mannopyranose | - |
Dyadobacter fermentans ATCC 700827 / DSM 18053 / NS114 | beta-D-mannopyranosyl-(1->4)-D-glucose | - |
? | |
| 4-O-beta-D-mannopyranosyl-beta-D-mannopyranose | - |
Flavobacterium johnsoniae ATCC 17061 / DSM 2064 / UW101 | beta-D-mannopyranosyl-(1->4)-D-glucose | - |
? | |
| 4-O-beta-D-mannopyranosyl-beta-D-mannopyranose | - |
Dictyoglomus turgidum Z-1310 / DSM 6724 | beta-D-mannopyranosyl-(1->4)-D-glucose | - |
? | |
| 4-O-beta-D-mannopyranosyl-beta-D-mannopyranose | - |
Spirochaeta thermophila ATCC 700085 / DSM 6578 / Z-1203 | beta-D-mannopyranosyl-(1->4)-D-glucose | - |
? | |
| cellobiose | - |
Ruminococcus albus | 4-O-beta-D-glucopyranosyl-D-mannose | - |
r | |
| cellobiose | - |
[Eubacterium] cellulosolvens | 4-O-beta-D-glucopyranosyl-D-mannose | - |
r | |
| cellobiose | - |
Bacteroides fragilis | 4-O-beta-D-glucopyranosyl-D-mannose | - |
r | |
| cellobiose | - |
Caldicellulosiruptor saccharolyticus | 4-O-beta-D-glucopyranosyl-D-mannose | - |
r | |
| cellobiose | - |
Rhodothermus marinus | 4-O-beta-D-glucopyranosyl-D-mannose | - |
r | |
| cellobiose | - |
Dyadobacter fermentans | 4-O-beta-D-glucopyranosyl-D-mannose | - |
r | |
| cellobiose | - |
Dictyoglomus turgidum | 4-O-beta-D-glucopyranosyl-D-mannose | - |
r | |
| cellobiose | - |
Flavobacterium johnsoniae | 4-O-beta-D-glucopyranosyl-D-mannose | - |
r | |
| cellobiose | - |
Herpetosiphon aurantiacus | 4-O-beta-D-glucopyranosyl-D-mannose | - |
r | |
| cellobiose | - |
Pedobacter heparinus | 4-O-beta-D-glucopyranosyl-D-mannose | - |
r | |
| cellobiose | - |
Saccharophagus degradans | 4-O-beta-D-glucopyranosyl-D-mannose | - |
r | |
| cellobiose | - |
Spirosoma linguale | 4-O-beta-D-glucopyranosyl-D-mannose | - |
r | |
| cellobiose | - |
Spirochaeta thermophila | 4-O-beta-D-glucopyranosyl-D-mannose | - |
r | |
| cellobiose | - |
Teredinibacter turnerae | 4-O-beta-D-glucopyranosyl-D-mannose | - |
r | |
| cellobiose | - |
Cellvibrio vulgaris | 4-O-beta-D-glucopyranosyl-D-mannose | - |
r | |
| cellobiose | - |
Bacteroides fragilis ATCC 25285 / DSM 2151 / JCM 11019 / NCTC 9343 | 4-O-beta-D-glucopyranosyl-D-mannose | - |
r | |
| cellobiose | - |
Spirochaeta thermophila ATCC 49972 / DSM 6192 / RI 19.B1 | 4-O-beta-D-glucopyranosyl-D-mannose | - |
r | |
| cellobiose | - |
Caldicellulosiruptor saccharolyticus ATCC 43494 / DSM 8903 / Tp8T 6331 | 4-O-beta-D-glucopyranosyl-D-mannose | - |
r | |
| cellobiose | - |
Herpetosiphon aurantiacus ATCC 23779 / DSM 785 | 4-O-beta-D-glucopyranosyl-D-mannose | - |
r | |
| cellobiose | - |
Spirosoma linguale ATCC 33905 / DSM 74 / LMG 10896 | 4-O-beta-D-glucopyranosyl-D-mannose | - |
r | |
| cellobiose | - |
Pedobacter heparinus ATCC 13125 / DSM 2366 / CIP 104194 / JCM 7457 / NBRC 12017 / NCIMB 9290 / NRRL B-14731 / HIM 762-3 | 4-O-beta-D-glucopyranosyl-D-mannose | - |
r | |
| cellobiose | - |
Saccharophagus degradans 2-40 / ATCC 43961 / DSM 17024 | 4-O-beta-D-glucopyranosyl-D-mannose | - |
r | |
| cellobiose | - |
Teredinibacter turnerae ATCC 39867 / T7901 | 4-O-beta-D-glucopyranosyl-D-mannose | - |
r | |
| cellobiose | - |
Dyadobacter fermentans ATCC 700827 / DSM 18053 / NS114 | 4-O-beta-D-glucopyranosyl-D-mannose | - |
r | |
| cellobiose | - |
Flavobacterium johnsoniae ATCC 17061 / DSM 2064 / UW101 | 4-O-beta-D-glucopyranosyl-D-mannose | - |
r | |
| cellobiose | - |
Dictyoglomus turgidum Z-1310 / DSM 6724 | 4-O-beta-D-glucopyranosyl-D-mannose | - |
r | |
| cellobiose | - |
Spirochaeta thermophila ATCC 700085 / DSM 6578 / Z-1203 | 4-O-beta-D-glucopyranosyl-D-mannose | - |
r | |
| cellotetraose | - |
Ruminococcus albus | beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-D-mannose | - |
r | |
| cellotriose | - |
Ruminococcus albus | beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-D-mannose | - |
r | |
| lactose | - |
Ruminococcus albus | epilactose | - |
r | |
| lactose | - |
[Eubacterium] cellulosolvens | epilactose | - |
r | |
| lactose | - |
Bacteroides fragilis | epilactose | - |
r | |
| lactose | - |
Caldicellulosiruptor saccharolyticus | epilactose | - |
r | |
| lactose | - |
Rhodothermus marinus | epilactose | - |
r | |
| lactose | - |
Dyadobacter fermentans | epilactose | - |
r | |
| lactose | - |
Dictyoglomus turgidum | epilactose | - |
r | |
| lactose | - |
Flavobacterium johnsoniae | epilactose | - |
r | |
| lactose | - |
Herpetosiphon aurantiacus | epilactose | - |
r | |
| lactose | - |
Pedobacter heparinus | epilactose | - |
r | |
| lactose | - |
Saccharophagus degradans | epilactose | - |
r | |
| lactose | - |
Spirosoma linguale | epilactose | - |
r | |
| lactose | - |
Spirochaeta thermophila | epilactose | - |
r | |
| lactose | - |
Teredinibacter turnerae | epilactose | - |
r | |
| lactose | - |
Cellvibrio vulgaris | epilactose | - |
r | |
| lactose | - |
Bacteroides fragilis ATCC 25285 / DSM 2151 / JCM 11019 / NCTC 9343 | epilactose | - |
r | |
| lactose | - |
Spirochaeta thermophila ATCC 49972 / DSM 6192 / RI 19.B1 | epilactose | - |
r | |
| lactose | - |
Caldicellulosiruptor saccharolyticus ATCC 43494 / DSM 8903 / Tp8T 6331 | epilactose | - |
r | |
| lactose | - |
Herpetosiphon aurantiacus ATCC 23779 / DSM 785 | epilactose | - |
r | |
| lactose | - |
Spirosoma linguale ATCC 33905 / DSM 74 / LMG 10896 | epilactose | - |
r | |
| lactose | - |
Pedobacter heparinus ATCC 13125 / DSM 2366 / CIP 104194 / JCM 7457 / NBRC 12017 / NCIMB 9290 / NRRL B-14731 / HIM 762-3 | epilactose | - |
r | |
| lactose | - |
Saccharophagus degradans 2-40 / ATCC 43961 / DSM 17024 | epilactose | - |
r | |
| lactose | - |
Teredinibacter turnerae ATCC 39867 / T7901 | epilactose | - |
r | |
| lactose | - |
Dyadobacter fermentans ATCC 700827 / DSM 18053 / NS114 | epilactose | - |
r | |
| lactose | - |
Flavobacterium johnsoniae ATCC 17061 / DSM 2064 / UW101 | epilactose | - |
r | |
| lactose | - |
Dictyoglomus turgidum Z-1310 / DSM 6724 | epilactose | - |
r | |
| lactose | - |
Spirochaeta thermophila ATCC 700085 / DSM 6578 / Z-1203 | epilactose | - |
r | |
| additional information | the enzyme catalyzes the epimerization of a D-glucose residue at the reducing end of cellobiose to a D-mannose residue, i.e. cellobiose is converted to beta-D-glucopyranosyl-(1->4)-D-mannose. Cellobiose isomerase is the sole enzyme catalyzing the epimerization of oligosaccharides among known carbohydrate isomerases/epimerases. The enzyme also has epimerization activity toward cellotriose, cellotetraose, lactose, and beta-(1->4)-mannobiose (Manbeta1->4Man). beta-D-Glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-D-mannose, beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-D-mannose, epilactose [beta-D-galactopyranosyl-(1->4)-D-mannose], and beta-D-mannopyranosyl-(1->4)-D-glucose (Manbeta1->4Glc) are generated from cellotriose, cellotetraose, lactose, and Manbeta1->4Man, respectively. In the reactions with lactose and cellobiose, the conversion levels of epilactose and Glcbeta1->4Man are both approximately 30%. Among beta-(1->4)-linked disaccharides, Manbeta1->4Man is the best substrate for RaCE in terms of catalytic efficiency. No epimerization activity of RaCE is detectable toward monosaccharides (N-acetyl-D-glucosamine, uridine 5'-diphosphate-D-glucose, D-glucose 6-phosphate, D-glucose, and D-mannose) or glucobioses linked other than by a beta-(1->4)-linkage [e.g. maltose: alpha-(1->4)-linkage, sophorose: beta-(1->2)-linkage, laminaribiose: beta-(1->3)-linkage, or gentiobiose: beta-(1->6)-linkage]. Thus enzyme RaCE is highly specific to a beta-(1->4)-linkage at the reducing end of substrates, but can catalyze the conversion of substrates with a D-glucosyl, D-mannosyl, or D-galactosyl residue next to the reducing end sugar residue | Ruminococcus albus | ? | - |
? | |
| additional information | the enzyme catalyzes the epimerization of a D-glucose residue at the reducing end of cellobiose to a D-mannose residue, i.e. cellobiose is converted to beta-D-glucopyranosyl-(1->4)-D-mannose. Enzyme cellobiose 2-epimerase, CE, is highly specific for disaccharides | [Eubacterium] cellulosolvens | ? | - |
? | |
| additional information | the enzyme catalyzes the epimerization of a D-glucose residue at the reducing end of cellobiose to a D-mannose residue, i.e. cellobiose is converted to beta-D-glucopyranosyl-(1->4)-D-mannose. Enzyme cellobiose 2-epimerase, CE, is highly specific for disaccharides | Bacteroides fragilis | ? | - |
? | |
| additional information | the enzyme catalyzes the epimerization of a D-glucose residue at the reducing end of cellobiose to a D-mannose residue, i.e. cellobiose is converted to beta-D-glucopyranosyl-(1->4)-D-mannose. Enzyme cellobiose 2-epimerase, CE, is highly specific for disaccharides | Caldicellulosiruptor saccharolyticus | ? | - |
? | |
| additional information | the enzyme catalyzes the epimerization of a D-glucose residue at the reducing end of cellobiose to a D-mannose residue, i.e. cellobiose is converted to beta-D-glucopyranosyl-(1->4)-D-mannose. Enzyme cellobiose 2-epimerase, CE, is highly specific for disaccharides | Rhodothermus marinus | ? | - |
? | |
| additional information | the enzyme catalyzes the epimerization of a D-glucose residue at the reducing end of cellobiose to a D-mannose residue, i.e. cellobiose is converted to beta-D-glucopyranosyl-(1->4)-D-mannose. Enzyme cellobiose 2-epimerase, CE, is highly specific for disaccharides | Dyadobacter fermentans | ? | - |
? | |
| additional information | the enzyme catalyzes the epimerization of a D-glucose residue at the reducing end of cellobiose to a D-mannose residue, i.e. cellobiose is converted to beta-D-glucopyranosyl-(1->4)-D-mannose. Enzyme cellobiose 2-epimerase, CE, is highly specific for disaccharides | Dictyoglomus turgidum | ? | - |
? | |
| additional information | the enzyme catalyzes the epimerization of a D-glucose residue at the reducing end of cellobiose to a D-mannose residue, i.e. cellobiose is converted to beta-D-glucopyranosyl-(1->4)-D-mannose. Enzyme cellobiose 2-epimerase, CE, is highly specific for disaccharides | Flavobacterium johnsoniae | ? | - |
? | |
| additional information | the enzyme catalyzes the epimerization of a D-glucose residue at the reducing end of cellobiose to a D-mannose residue, i.e. cellobiose is converted to beta-D-glucopyranosyl-(1->4)-D-mannose. Enzyme cellobiose 2-epimerase, CE, is highly specific for disaccharides | Herpetosiphon aurantiacus | ? | - |
? | |
| additional information | the enzyme catalyzes the epimerization of a D-glucose residue at the reducing end of cellobiose to a D-mannose residue, i.e. cellobiose is converted to beta-D-glucopyranosyl-(1->4)-D-mannose. Enzyme cellobiose 2-epimerase, CE, is highly specific for disaccharides | Pedobacter heparinus | ? | - |
? | |
| additional information | the enzyme catalyzes the epimerization of a D-glucose residue at the reducing end of cellobiose to a D-mannose residue, i.e. cellobiose is converted to beta-D-glucopyranosyl-(1->4)-D-mannose. Enzyme cellobiose 2-epimerase, CE, is highly specific for disaccharides | Saccharophagus degradans | ? | - |
? | |
| additional information | the enzyme catalyzes the epimerization of a D-glucose residue at the reducing end of cellobiose to a D-mannose residue, i.e. cellobiose is converted to beta-D-glucopyranosyl-(1->4)-D-mannose. Enzyme cellobiose 2-epimerase, CE, is highly specific for disaccharides | Spirosoma linguale | ? | - |
? | |
| additional information | the enzyme catalyzes the epimerization of a D-glucose residue at the reducing end of cellobiose to a D-mannose residue, i.e. cellobiose is converted to beta-D-glucopyranosyl-(1->4)-D-mannose. Enzyme cellobiose 2-epimerase, CE, is highly specific for disaccharides | Spirochaeta thermophila | ? | - |
? | |
| additional information | the enzyme catalyzes the epimerization of a D-glucose residue at the reducing end of cellobiose to a D-mannose residue, i.e. cellobiose is converted to beta-D-glucopyranosyl-(1->4)-D-mannose. Enzyme cellobiose 2-epimerase, CE, is highly specific for disaccharides | Teredinibacter turnerae | ? | - |
? | |
| additional information | the enzyme catalyzes the epimerization of a D-glucose residue at the reducing end of cellobiose to a D-mannose residue, i.e. cellobiose is converted to beta-D-glucopyranosyl-(1->4)-D-mannose. Enzyme cellobiose 2-epimerase, CE, is highly specific for disaccharides | Cellvibrio vulgaris | ? | - |
? | |
| additional information | the enzyme catalyzes the epimerization of a D-glucose residue at the reducing end of cellobiose to a D-mannose residue, i.e. cellobiose is converted to beta-D-glucopyranosyl-(1->4)-D-mannose. Enzyme cellobiose 2-epimerase, CE, is highly specific for disaccharides | Bacteroides fragilis ATCC 25285 / DSM 2151 / JCM 11019 / NCTC 9343 | ? | - |
? | |
| additional information | the enzyme catalyzes the epimerization of a D-glucose residue at the reducing end of cellobiose to a D-mannose residue, i.e. cellobiose is converted to beta-D-glucopyranosyl-(1->4)-D-mannose. Enzyme cellobiose 2-epimerase, CE, is highly specific for disaccharides | Spirochaeta thermophila ATCC 49972 / DSM 6192 / RI 19.B1 | ? | - |
? | |
| additional information | the enzyme catalyzes the epimerization of a D-glucose residue at the reducing end of cellobiose to a D-mannose residue, i.e. cellobiose is converted to beta-D-glucopyranosyl-(1->4)-D-mannose. Enzyme cellobiose 2-epimerase, CE, is highly specific for disaccharides | Caldicellulosiruptor saccharolyticus ATCC 43494 / DSM 8903 / Tp8T 6331 | ? | - |
? | |
| additional information | the enzyme catalyzes the epimerization of a D-glucose residue at the reducing end of cellobiose to a D-mannose residue, i.e. cellobiose is converted to beta-D-glucopyranosyl-(1->4)-D-mannose. Enzyme cellobiose 2-epimerase, CE, is highly specific for disaccharides | Herpetosiphon aurantiacus ATCC 23779 / DSM 785 | ? | - |
? | |
| additional information | the enzyme catalyzes the epimerization of a D-glucose residue at the reducing end of cellobiose to a D-mannose residue, i.e. cellobiose is converted to beta-D-glucopyranosyl-(1->4)-D-mannose. Enzyme cellobiose 2-epimerase, CE, is highly specific for disaccharides | Spirosoma linguale ATCC 33905 / DSM 74 / LMG 10896 | ? | - |
? | |
| additional information | the enzyme catalyzes the epimerization of a D-glucose residue at the reducing end of cellobiose to a D-mannose residue, i.e. cellobiose is converted to beta-D-glucopyranosyl-(1->4)-D-mannose. Enzyme cellobiose 2-epimerase, CE, is highly specific for disaccharides | Pedobacter heparinus ATCC 13125 / DSM 2366 / CIP 104194 / JCM 7457 / NBRC 12017 / NCIMB 9290 / NRRL B-14731 / HIM 762-3 | ? | - |
? | |
| additional information | the enzyme catalyzes the epimerization of a D-glucose residue at the reducing end of cellobiose to a D-mannose residue, i.e. cellobiose is converted to beta-D-glucopyranosyl-(1->4)-D-mannose. Enzyme cellobiose 2-epimerase, CE, is highly specific for disaccharides | Saccharophagus degradans 2-40 / ATCC 43961 / DSM 17024 | ? | - |
? | |
| additional information | the enzyme catalyzes the epimerization of a D-glucose residue at the reducing end of cellobiose to a D-mannose residue, i.e. cellobiose is converted to beta-D-glucopyranosyl-(1->4)-D-mannose. Enzyme cellobiose 2-epimerase, CE, is highly specific for disaccharides | Teredinibacter turnerae ATCC 39867 / T7901 | ? | - |
? | |
| additional information | the enzyme catalyzes the epimerization of a D-glucose residue at the reducing end of cellobiose to a D-mannose residue, i.e. cellobiose is converted to beta-D-glucopyranosyl-(1->4)-D-mannose. Enzyme cellobiose 2-epimerase, CE, is highly specific for disaccharides | Dyadobacter fermentans ATCC 700827 / DSM 18053 / NS114 | ? | - |
? | |
| additional information | the enzyme catalyzes the epimerization of a D-glucose residue at the reducing end of cellobiose to a D-mannose residue, i.e. cellobiose is converted to beta-D-glucopyranosyl-(1->4)-D-mannose. Enzyme cellobiose 2-epimerase, CE, is highly specific for disaccharides | Flavobacterium johnsoniae ATCC 17061 / DSM 2064 / UW101 | ? | - |
? | |
| additional information | the enzyme catalyzes the epimerization of a D-glucose residue at the reducing end of cellobiose to a D-mannose residue, i.e. cellobiose is converted to beta-D-glucopyranosyl-(1->4)-D-mannose. Enzyme cellobiose 2-epimerase, CE, is highly specific for disaccharides | Dictyoglomus turgidum Z-1310 / DSM 6724 | ? | - |
? | |
| additional information | the enzyme catalyzes the epimerization of a D-glucose residue at the reducing end of cellobiose to a D-mannose residue, i.e. cellobiose is converted to beta-D-glucopyranosyl-(1->4)-D-mannose. Enzyme cellobiose 2-epimerase, CE, is highly specific for disaccharides | Spirochaeta thermophila ATCC 700085 / DSM 6578 / Z-1203 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 43000, SDS-PAGE | Ruminococcus albus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| BfCE | - |
Bacteroides fragilis |
| CE-NE1 | - |
Ruminococcus albus |
| ce13 | - |
[Eubacterium] cellulosolvens |
| cellobiose 2-epimerase | - |
Ruminococcus albus |
| cellobiose 2-epimerase | - |
[Eubacterium] cellulosolvens |
| cellobiose 2-epimerase | - |
Bacteroides fragilis |
| cellobiose 2-epimerase | - |
Caldicellulosiruptor saccharolyticus |
| cellobiose 2-epimerase | - |
Rhodothermus marinus |
| cellobiose 2-epimerase | - |
Dyadobacter fermentans |
| cellobiose 2-epimerase | - |
Dictyoglomus turgidum |
| cellobiose 2-epimerase | - |
Flavobacterium johnsoniae |
| cellobiose 2-epimerase | - |
Herpetosiphon aurantiacus |
| cellobiose 2-epimerase | - |
Pedobacter heparinus |
| cellobiose 2-epimerase | - |
Saccharophagus degradans |
| cellobiose 2-epimerase | - |
Spirosoma linguale |
| cellobiose 2-epimerase | - |
Spirochaeta thermophila |
| cellobiose 2-epimerase | - |
Teredinibacter turnerae |
| cellobiose 2-epimerase | - |
Cellvibrio vulgaris |
| Csac_0294 | - |
Caldicellulosiruptor saccharolyticus |
| CsCE | - |
Caldicellulosiruptor saccharolyticus |
| CvCE | - |
Cellvibrio vulgaris |
| DfCE | - |
Dyadobacter fermentans |
| Dfer_1975 | - |
Dyadobacter fermentans |
| DtCE | - |
Dictyoglomus turgidum |
| Dtur_0652 | - |
Dictyoglomus turgidum |
| EcCE | - |
[Eubacterium] cellulosolvens |
| FjCE | - |
Flavobacterium johnsoniae |
| Fjoh_4956 | - |
Flavobacterium johnsoniae |
| HaCE | - |
Herpetosiphon aurantiacus |
| Haur_0887 | - |
Herpetosiphon aurantiacus |
| PhCE | - |
Pedobacter heparinus |
| Phep_3983 | - |
Pedobacter heparinus |
| RACE | - |
Ruminococcus albus |
| RACE | - |
Bacteroides fragilis |
| RmCE | - |
Rhodothermus marinus |
| SdCE | - |
Saccharophagus degradans |
| Sde_0508 | - |
Saccharophagus degradans |
| SlCE | - |
Spirosoma linguale |
| Slin_0271 | - |
Spirosoma linguale |
| Spith_0082 | - |
Spirochaeta thermophila |
| StCE | - |
Spirochaeta thermophila |
| STHERM_c00950 | - |
Spirochaeta thermophila |
| TERTU_4095 | - |
Teredinibacter turnerae |
| TtCE | - |
Teredinibacter turnerae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Ruminococcus albus |
| 35 | - |
- |
[Eubacterium] cellulosolvens |
| 35 | - |
- |
Flavobacterium johnsoniae |
| 35 | - |
- |
Pedobacter heparinus |
| 35 | - |
- |
Saccharophagus degradans |
| 35 | - |
- |
Teredinibacter turnerae |
| 40 | - |
- |
Cellvibrio vulgaris |
| 45 | - |
- |
Bacteroides fragilis |
| 45 | - |
- |
Herpetosiphon aurantiacus |
| 45 | - |
- |
Spirosoma linguale |
| 50 | - |
- |
Dyadobacter fermentans |
| 60 | - |
- |
Spirochaeta thermophila |
| 70 | - |
- |
Dictyoglomus turgidum |
| 75 | - |
- |
Caldicellulosiruptor saccharolyticus |
| 80 | - |
- |
Rhodothermus marinus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
stable up to | Flavobacterium johnsoniae |
| 30 | - |
stable up to | Pedobacter heparinus |
| 30 | - |
stable up to | Saccharophagus degradans |
| 40 | - |
stable up to | [Eubacterium] cellulosolvens |
| 40 | - |
stable up to | Cellvibrio vulgaris |
| 45 | - |
stable up to | Teredinibacter turnerae |
| 50 | - |
stable up to | Dyadobacter fermentans |
| 50 | - |
stable up to | Herpetosiphon aurantiacus |
| 50 | - |
stable up to | Spirosoma linguale |
| 60 | - |
stable up to | Spirochaeta thermophila |
| 80 | - |
stable up to | Caldicellulosiruptor saccharolyticus |
| 80 | - |
stable up to | Rhodothermus marinus |
| 80 | - |
stable up to | Dictyoglomus turgidum |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2 | 8 | cellobiose | pH 7.5, 30°C, recombinant enzyme | Spirochaeta thermophila | |
| 5.43 | - |
lactose | pH 7.5, 30°C, recombinant enzyme | Pedobacter heparinus | |
| 7.02 | - |
cellobiose | pH 7.5, 30°C, recombinant enzyme | Pedobacter heparinus | |
| 7.82 | - |
lactose | pH 7.5, 30°C, recombinant enzyme | Saccharophagus degradans | |
| 14 | - |
lactose | pH 7.5, 30°C, recombinant enzyme | Herpetosiphon aurantiacus | |
| 17.5 | - |
lactose | pH 7.5, 30°C, recombinant enzyme | Flavobacterium johnsoniae | |
| 18.6 | - |
lactose | pH 7.5, 30°C, recombinant enzyme | Cellvibrio vulgaris | |
| 18.7 | - |
cellobiose | pH 7.5, 30°C, recombinant enzyme | Herpetosiphon aurantiacus | |
| 26.1 | - |
cellobiose | pH 7.5, 30°C, recombinant enzyme | Saccharophagus degradans | |
| 28.5 | - |
cellobiose | pH 7.5, 30°C, recombinant enzyme | [Eubacterium] cellulosolvens | |
| 32.5 | - |
lactose | pH 7.5, 30°C, recombinant enzyme | [Eubacterium] cellulosolvens | |
| 35.8 | - |
4-O-beta-D-mannopyranosyl-beta-D-mannopyranose | pH 7.5, 30°C, recombinant enzyme | Spirochaeta thermophila | |
| 38.8 | - |
cellobiose | pH 7.5, 30°C, recombinant enzyme | Cellvibrio vulgaris | |
| 39.9 | - |
cellobiose | pH 7.5, 30°C, recombinant enzyme | Flavobacterium johnsoniae | |
| 44.9 | - |
lactose | pH 7.5, 30°C, recombinant enzyme | Dyadobacter fermentans | |
| 52.1 | - |
lactose | pH 7.5, 30°C, recombinant enzyme | Ruminococcus albus | |
| 63.8 | - |
cellobiose | pH 7.5, 30°C, recombinant enzyme | Ruminococcus albus | |
| 67.6 | - |
cellobiose | pH 7.5, 30°C, recombinant enzyme | Bacteroides fragilis | |
| 79.5 | - |
lactose | pH 7.5, 30°C, recombinant enzyme | Bacteroides fragilis | |
| 80.8 | - |
cellobiose | pH 7.5, 30°C, recombinant enzyme | Rhodothermus marinus | |
| 89.4 | - |
lactose | pH 7.5, 30°C, recombinant enzyme | Dictyoglomus turgidum | |
| 92.1 | - |
lactose | pH 7.5, 30°C, recombinant enzyme | Spirosoma linguale | |
| 102 | - |
4-O-beta-D-mannopyranosyl-beta-D-mannopyranose | pH 7.5, 30°C, recombinant enzyme | Rhodothermus marinus | |
| 102 | - |
4-O-beta-D-mannopyranosyl-beta-D-mannopyranose | pH 7.5, 30°C, recombinant enzyme | Cellvibrio vulgaris | |
| 104 | - |
4-O-beta-D-mannopyranosyl-beta-D-mannopyranose | pH 7.5, 30°C, recombinant enzyme | Bacteroides fragilis | |
| 111 | - |
lactose | pH 7.5, 30°C, recombinant enzyme | Rhodothermus marinus | |
| 165 | - |
cellobiose | pH 7.5, 30°C, recombinant enzyme | Teredinibacter turnerae | |
| 169 | - |
cellobiose | pH 7.5, 30°C, recombinant enzyme | Dictyoglomus turgidum | |
| 175 | - |
lactose | pH 7.5, 30°C, recombinant enzyme | Teredinibacter turnerae | |
| 201 | - |
4-O-beta-D-mannopyranosyl-beta-D-mannopyranose | pH 7.5, 30°C, recombinant enzyme | Ruminococcus albus | |
| 222 | - |
cellobiose | pH 7.5, 30°C, recombinant enzyme | Spirosoma linguale | |
| 240 | - |
cellobiose | pH 7.5, 30°C, recombinant enzyme | Dyadobacter fermentans | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.3 | - |
- |
Rhodothermus marinus |
| 6.3 | - |
- |
Pedobacter heparinus |
| 7 | - |
- |
Dictyoglomus turgidum |
| 7 | - |
- |
Spirochaeta thermophila |
| 7.3 | - |
- |
Herpetosiphon aurantiacus |
| 7.5 | - |
- |
Ruminococcus albus |
| 7.5 | - |
- |
[Eubacterium] cellulosolvens |
| 7.5 | - |
- |
Bacteroides fragilis |
| 7.5 | - |
- |
Caldicellulosiruptor saccharolyticus |
| 7.7 | - |
- |
Dyadobacter fermentans |
| 7.7 | - |
- |
Saccharophagus degradans |
| 7.7 | - |
- |
Spirosoma linguale |
| 8 | - |
- |
Cellvibrio vulgaris |
| 8.4 | - |
- |
Flavobacterium johnsoniae |
| 8.8 | - |
- |
Teredinibacter turnerae |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 2.2 | 9.5 | stable at | Spirosoma linguale |
| 3.2 | 10.8 | stable at | Rhodothermus marinus |
| 3.2 | 10.2 | stable at | Dyadobacter fermentans |
| 3.4 | 10.2 | stable at | Teredinibacter turnerae |
| 4 | 8 | stable at | [Eubacterium] cellulosolvens |
| 4.7 | 10.8 | stable at | Saccharophagus degradans |
| 4.7 | 9.8 | stable at | Flavobacterium johnsoniae |
| 5.2 | 10 | stable at | Cellvibrio vulgaris |
| 5.3 | 11.8 | stable at | Pedobacter heparinus |
| 8 | 9.4 | stable at | Herpetosiphon aurantiacus |
General Information
| General Information | Comment | Organism |
|---|---|---|
| additional information | enzyme structure-function relationship, overview | Ruminococcus albus |
| additional information | enzyme structure-function relationship, overview | [Eubacterium] cellulosolvens |
| additional information | enzyme structure-function relationship, overview | Bacteroides fragilis |
| additional information | enzyme structure-function relationship, overview | Caldicellulosiruptor saccharolyticus |
| additional information | enzyme structure-function relationship, overview | Rhodothermus marinus |
| additional information | enzyme structure-function relationship, overview | Dyadobacter fermentans |
| additional information | enzyme structure-function relationship, overview | Dictyoglomus turgidum |
| additional information | enzyme structure-function relationship, overview | Flavobacterium johnsoniae |
| additional information | enzyme structure-function relationship, overview | Herpetosiphon aurantiacus |
| additional information | enzyme structure-function relationship, overview | Pedobacter heparinus |
| additional information | enzyme structure-function relationship, overview | Saccharophagus degradans |
| additional information | enzyme structure-function relationship, overview | Spirosoma linguale |
| additional information | enzyme structure-function relationship, overview | Spirochaeta thermophila |
| additional information | enzyme structure-function relationship, overview | Teredinibacter turnerae |
| additional information | enzyme structure-function relationship, overview | Cellvibrio vulgaris |
| physiological function | physiological function of cellobiose 2-epimerase in carbohydrate metabolism, overview | Ruminococcus albus |
| physiological function | physiological function of cellobiose 2-epimerase in carbohydrate metabolism, overview | [Eubacterium] cellulosolvens |
| physiological function | physiological function of cellobiose 2-epimerase in carbohydrate metabolism, overview | Bacteroides fragilis |
| physiological function | physiological function of cellobiose 2-epimerase in carbohydrate metabolism, overview | Caldicellulosiruptor saccharolyticus |
| physiological function | physiological function of cellobiose 2-epimerase in carbohydrate metabolism, overview | Rhodothermus marinus |
| physiological function | physiological function of cellobiose 2-epimerase in carbohydrate metabolism, overview | Dyadobacter fermentans |
| physiological function | physiological function of cellobiose 2-epimerase in carbohydrate metabolism, overview | Dictyoglomus turgidum |
| physiological function | physiological function of cellobiose 2-epimerase in carbohydrate metabolism, overview | Flavobacterium johnsoniae |
| physiological function | physiological function of cellobiose 2-epimerase in carbohydrate metabolism, overview | Herpetosiphon aurantiacus |
| physiological function | physiological function of cellobiose 2-epimerase in carbohydrate metabolism, overview | Pedobacter heparinus |
| physiological function | physiological function of cellobiose 2-epimerase in carbohydrate metabolism, overview | Saccharophagus degradans |
| physiological function | physiological function of cellobiose 2-epimerase in carbohydrate metabolism, overview | Spirosoma linguale |
| physiological function | physiological function of cellobiose 2-epimerase in carbohydrate metabolism, overview | Spirochaeta thermophila |
| physiological function | physiological function of cellobiose 2-epimerase in carbohydrate metabolism, overview | Teredinibacter turnerae |
| physiological function | physiological function of cellobiose 2-epimerase in carbohydrate metabolism, overview | Cellvibrio vulgaris |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.222 | - |
lactose | pH 7.5, 30°C, recombinant enzyme | Pedobacter heparinus | |
| 0.237 | - |
cellobiose | pH 7.5, 30°C, recombinant enzyme | Pedobacter heparinus | |
| 0.268 | - |
lactose | pH 7.5, 30°C, recombinant enzyme | Saccharophagus degradans | |
| 0.27 | - |
lactose | pH 7.5, 30°C, recombinant enzyme | Herpetosiphon aurantiacus | |
| 0.447 | - |
lactose | pH 7.5, 30°C, recombinant enzyme | Spirosoma linguale | |
| 0.451 | - |
lactose | pH 7.5, 30°C, recombinant enzyme | [Eubacterium] cellulosolvens | |
| 0.469 | - |
lactose | pH 7.5, 30°C, recombinant enzyme | Dyadobacter fermentans | |
| 0.501 | - |
lactose | pH 7.5, 30°C, recombinant enzyme | Flavobacterium johnsoniae | |
| 0.648 | - |
lactose | pH 7.5, 30°C, recombinant enzyme | Cellvibrio vulgaris | |
| 0.663 | - |
cellobiose | pH 7.5, 30°C, recombinant enzyme | Herpetosiphon aurantiacus | |
| 0.735 | - |
lactose | pH 7.5, 30°C, recombinant enzyme | Teredinibacter turnerae | |
| 0.75 | - |
cellobiose | pH 7.5, 30°C, recombinant enzyme | Flavobacterium johnsoniae | |
| 0.833 | - |
cellobiose | pH 7.5, 30°C, recombinant enzyme | Teredinibacter turnerae | |
| 1.12 | - |
lactose | pH 7.5, 30°C, recombinant enzyme | Dictyoglomus turgidum | |
| 1.15 | - |
cellobiose | pH 7.5, 30°C, recombinant enzyme | Saccharophagus degradans | |
| 1.34 | - |
cellobiose | pH 7.5, 30°C, recombinant enzyme | Dyadobacter fermentans | |
| 1.58 | - |
cellobiose | pH 7.5, 30°C, recombinant enzyme | Cellvibrio vulgaris | |
| 1.58 | - |
lactose | pH 7.5, 30°C, recombinant enzyme | Ruminococcus albus | |
| 2.13 | - |
cellobiose | pH 7.5, 30°C, recombinant enzyme | Spirosoma linguale | |
| 2.23 | - |
cellobiose | pH 7.5, 30°C, recombinant enzyme | Dictyoglomus turgidum | |
| 2.52 | - |
cellobiose | pH 7.5, 30°C, recombinant enzyme | [Eubacterium] cellulosolvens | |
| 2.97 | - |
cellobiose | pH 7.5, 30°C, recombinant enzyme | Rhodothermus marinus | |
| 3.85 | - |
lactose | pH 7.5, 30°C, recombinant enzyme | Rhodothermus marinus | |
| 4.62 | - |
cellobiose | pH 7.5, 30°C, recombinant enzyme | Ruminococcus albus | |
| 6.51 | - |
cellobiose | pH 7.5, 30°C, recombinant enzyme | Spirochaeta thermophila | |
| 10.2 | - |
4-O-beta-D-mannopyranosyl-beta-D-mannopyranose | pH 7.5, 30°C, recombinant enzyme | Spirochaeta thermophila | |
| 12.1 | - |
lactose | pH 7.5, 30°C, recombinant enzyme | Bacteroides fragilis | |
| 18 | - |
cellobiose | pH 7.5, 30°C, recombinant enzyme | Bacteroides fragilis | |
| 18.9 | - |
4-O-beta-D-mannopyranosyl-beta-D-mannopyranose | pH 7.5, 30°C, recombinant enzyme | Bacteroides fragilis | |
| 19.4 | - |
4-O-beta-D-mannopyranosyl-beta-D-mannopyranose | pH 7.5, 30°C, recombinant enzyme | Rhodothermus marinus | |
| 23 | - |
4-O-beta-D-mannopyranosyl-beta-D-mannopyranose | pH 7.5, 30°C, recombinant enzyme | Ruminococcus albus | |
| 25.3 | - |
4-O-beta-D-mannopyranosyl-beta-D-mannopyranose | pH 7.5, 30°C, recombinant enzyme | Cellvibrio vulgaris | |
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Release 2023.1 (January 2023)
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