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Literature summary for 5.1.3.11 extracted from
- Efficient production of lactulose from whey powder by cellobiose 2-epimerase in an enzymatic membrane reactor (2017), Biores. Technol., 233, 305-312 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene Csac_0294, recombinant expression in Bacillus subtilis strain WB800, subcloning in Escherichia coli strain DH5alpha | Caldicellulosiruptor saccharolyticus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | efficient production of lactulose from whey powder, a by-product of cheese industry, by cellobiose 2-epimerase in an enzymatic membrane reactor using the recombinant enzyme, expressed in Bacillus subtilis, with great operational stability, method optimization and evaluation, overview. Optimal pH stability is displayed between pH 7.0 and 8.0. The transformation of whey powder solution into lactulose is conducted by 7.5 U/mg enzyme CsCE at 70°C in 50 mL 50 mM PBS, pH 7.0, with different concentration whey powder (lactose), 58.5% lactose is obtained from whey powder compared to 29.6% by the chemical method | Caldicellulosiruptor saccharolyticus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| lactose | Caldicellulosiruptor saccharolyticus | - |
epilactose | - |
r |  |
| lactose | Caldicellulosiruptor saccharolyticus ATCC 43494 / DSM 8903 / Tp8T 6331 | - |
epilactose | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Caldicellulosiruptor saccharolyticus | A4XGA6 | - |
- |
| Caldicellulosiruptor saccharolyticus ATCC 43494 / DSM 8903 / Tp8T 6331 | A4XGA6 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| lactose | - |
Caldicellulosiruptor saccharolyticus | epilactose | - |
r | |
| lactose | - |
Caldicellulosiruptor saccharolyticus ATCC 43494 / DSM 8903 / Tp8T 6331 | epilactose | - |
r | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| cellobiose 2-epimerase | - |
Caldicellulosiruptor saccharolyticus |
| CsCE | - |
Caldicellulosiruptor saccharolyticus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
assay at | Caldicellulosiruptor saccharolyticus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
purified recombinant enzyme, pH 7.0, 40 min, 80% of original activity is retained below 80°C | Caldicellulosiruptor saccharolyticus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Caldicellulosiruptor saccharolyticus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8 | at pH 6.5-7.5, the activity is over 80% of maximal activity, but decrease gradually at pH above pH 8.0 or below pH 6.0 | Caldicellulosiruptor saccharolyticus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 8 | purified recombinant enzyme, stable over a broad pH range, with nearly 80% of activity remaining at pH 5.0-8.5 after 40 min pre-treatment | Caldicellulosiruptor saccharolyticus |
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