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Literature summary for 5.1.3.11 extracted from

  • Wasaki, J.; Taguchi, H.; Senoura, T.; Akasaka, H.; Watanabe, J.; Kawaguchi, K.; Komata, Y.; Hanashiro, K.; Ito, S.
    Identification and distribution of cellobiose 2-epimerase genes by a PCR-based metagenomic approach (2015), Appl. Microbiol. Biotechnol., 99, 4287-4295 .
    View publication on PubMed
Search for more literature for 5.1.3.11

Cloned(Commentary)

Cloned (Comment) Organism
gene md1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis and tree, recombinant expression in Escherichia coli uncultured bacterium
gene md2, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis and tree, recombinant expression in Escherichia coli uncultured bacterium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.92
-
 cellobiose recombinant enzyme, pH 7.5, 25°C uncultured bacterium
10.8
-
 cellobiose recombinant enzyme, pH 7.5, 30°C uncultured bacterium
21.8
-
 mannobiose recombinant enzyme, pH 7.5, 30°C uncultured bacterium
25.1
-
 mannobiose recombinant enzyme, pH 7.5, 25°C uncultured bacterium

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cellobiose uncultured bacterium
-
 4-O-beta-D-glucopyranosyl-D-mannose
-
 r

Organism

Organism UniProt Comment Textmining
uncultured bacterium A0A0A1H8D7
-
-
uncultured bacterium A0A0A1HAT7
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Escherichia coli uncultured bacterium

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.7
-
 purified recombinant enzyme, pH 7.5, 25°C, substrate epilactose uncultured bacterium
9.06
-
 purified recombinant enzyme, pH 7.5, 25°C, substrate lactose uncultured bacterium
10.4
-
 purified recombinant enzyme, pH 7.5, 30°C, substrate lactose uncultured bacterium
21.1
-
 purified recombinant enzyme, pH 7.5, 30°C, substrate epilactose uncultured bacterium
62.3
-
 purified recombinant enzyme, pH 7.5, 25°C, substrate cellobiose uncultured bacterium
71.6
-
 purified recombinant enzyme, pH 7.5, 30°C, substrate cellobiose uncultured bacterium
97.5
-
 purified recombinant enzyme, pH 7.5, 30°C, substrate beta-1,4-mannobiose uncultured bacterium
201
-
 purified recombinant enzyme, pH 7.5, 25°C, substrate beta-1,4-mannobiose uncultured bacterium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cellobiose
-
 uncultured bacterium 4-O-beta-D-glucopyranosyl-D-mannose
-
 r
cellobiose (beta1,4) uncultured bacterium 4-O-beta-D-glucopyranosyl-D-mannose
-
 r
cellobiose (beta1,4), best substrate of md2 uncultured bacterium 4-O-beta-D-glucopyranosyl-D-mannose
-
 r
epilactose (beta1,4) uncultured bacterium lactose
-
 r
lactose (beta1,4) uncultured bacterium epilactose
-
 r
mannobiose (beta1,4) uncultured bacterium 4-O-beta-D-mannopyranosyl-D-glucose
-
 r
mannobiose (beta1,4), best substrate of md1 uncultured bacterium 4-O-beta-D-mannopyranosyl-D-glucose
-
 r
additional information substrate specificitiy of mD1 toward beta-linked disaccharides, overview. The enzyme is active on mannobiose (beta-1,4), cellobiose (beta-1,4), lactose (beta-1,4), and epilactose (beta-1,4), but not on laminaribiose (beta-1,3), gentiobiose (beta-1,6), and xylobiose (beta-1,4). No epimerization activity toward monosaccharides such as mannose, glucose, xylose, galactose, fructose, and GlcNAc and alpha-linked disaccharides such as trehalose (alpha-1,1), kojibiose (alpha-1,2), nigerose (alpha-1,3), maltose (alpha-1,4), sucrose (alpha-1,4), and melibiose (alpha-1,6). Enzyme mD1 favors mannobiose over cellobiose for catalysis uncultured bacterium ?
-
 ?
additional information substrate specificitiy of mD1 toward beta-linked disaccharides, overview. The enzyme is active on mannobiose (beta-1,4), cellobiose (beta-1,4), lactose (beta-1,4), and epilactose (beta-1,4), but not on laminaribiose (beta-1,3), gentiobiose (beta-1,6), and xylobiose (beta-1,4). No epimerization activity toward monosaccharides such as mannose, glucose, xylose, galactose, fructose, and GlcNAc and alpha-linked disaccharides such as trehalose (alpha-1,1), kojibiose (alpha-1,2), nigerose (alpha-1,3), maltose (alpha-1,4), sucrose (alpha-1,4), and melibiose (alpha-1,6). Enzyme mD2 favors mannobiose over cellobiose for catalysis uncultured bacterium ?
-
 ?

Subunits

Subunits Comment Organism
? x * 44800, recombinant enzyme, SDS-PAGE uncultured bacterium
? x * 46600, recombinant enzyme, SDS-PAGE uncultured bacterium

Synonyms

Synonyms Comment Organism
cellobiose 2-epimerase
-
 uncultured bacterium
md1
-
 uncultured bacterium
md2
-
 uncultured bacterium

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
-
 uncultured bacterium
30
-
-
 uncultured bacterium

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
109
-
 cellobiose recombinant enzyme, pH 7.5, 30°C uncultured bacterium
113
-
 mannobiose recombinant enzyme, pH 7.5, 25°C uncultured bacterium
137
-
 mannobiose recombinant enzyme, pH 7.5, 30°C uncultured bacterium
166
-
 cellobiose recombinant enzyme, pH 7.5, 25°C uncultured bacterium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 uncultured bacterium

General Information

General Information Comment Organism
evolution the cluster of CE and CE-like proteins is functionally and evolutionarily separated from that of N-acetyl-D-glucosamine 2-epimerase (AGE) and AGE-like proteins, phylogenetic analysis uncultured bacterium

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.5
-
 mannobiose recombinant enzyme, pH 7.5, 25°C uncultured bacterium
6.33
-
 mannobiose recombinant enzyme, pH 7.5, 30°C uncultured bacterium
11
-
 cellobiose recombinant enzyme, pH 7.5, 30°C uncultured bacterium
42.8
-
 cellobiose recombinant enzyme, pH 7.5, 25°C uncultured bacterium