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Literature summary for 5.1.3.11 extracted from
- Characterization of a recombinant cellobiose 2-epimerase from Dictyoglomus turgidum that epimerizes and isomerizes beta-1,4- and alpha-1,4-gluco-oligosaccharides (2012), Biotechnol. Lett., 34, 2061-2068.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of His-tagged enzyme in Escherichia coli | Dictyoglomus turgidum |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 75.8 | - |
cellobiose | purified recombinant His-tagged enzyme, pH 7.0, 70°C | Dictyoglomus turgidum |  |
| 79.6 | - |
lactose | purified recombinant His-tagged enzyme, pH 7.0, 70°C | Dictyoglomus turgidum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 47000 | - |
recombinant His-tagged enzyme, gel filtration | Dictyoglomus turgidum |
| 47200 | - |
1 * 47000, recombinant His-tagged enzyme, SDS-PAGE, 1 * 47200, about, recombinant His-tagged enzyme, sequence calculation | Dictyoglomus turgidum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cellobiose | Dictyoglomus turgidum | - |
D-glucosyl-D-mannose | - |
? | |
| cellobiose | Dictyoglomus turgidum DSM 6724 | - |
D-glucosyl-D-mannose | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Dictyoglomus turgidum | - |
- |
- |
| Dictyoglomus turgidum DSM 6724 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme 10fold from Escherichia coli by heat treatment and metal affinity chromatography | Dictyoglomus turgidum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 14.2 | - |
purified recombinant His-tagged enzyme, substrate lactose, pH 7.0, 70°C | Dictyoglomus turgidum |
| 16.8 | 24.4 | purified recombinant His-tagged enzyme, substrate cellolbiose, pH 7.0, 70°C | Dictyoglomus turgidum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cellobiose | - |
Dictyoglomus turgidum | 4-O-beta-D-glucopyranosyl-D-mannose | - |
? | |
| cellobiose | - |
Dictyoglomus turgidum DSM 6724 | 4-O-beta-D-glucopyranosyl-D-mannose | - |
? | |
| cellobiose | - |
Dictyoglomus turgidum | D-glucosyl-D-mannose | - |
? | |
| cellobiose | - |
Dictyoglomus turgidum DSM 6724 | D-glucosyl-D-mannose | - |
? | |
| cellotriose | - |
Dictyoglomus turgidum | 4-O-beta-D-glucopyranosyl-4-O-beta-D-glucopyranosyl-D-mannose | - |
? | |
| D-glucose | - |
Dictyoglomus turgidum | D-mannose | - |
? | |
| D-glucose | - |
Dictyoglomus turgidum DSM 6724 | D-mannose | - |
? | |
| lactose | - |
Dictyoglomus turgidum | epilactose | - |
? | |
| lactose | - |
Dictyoglomus turgidum DSM 6724 | epilactose | - |
? | |
| lactose | - |
Dictyoglomus turgidum | lactulose | - |
? | |
| maltose | - |
Dictyoglomus turgidum | 4-O-alpha-D-glucopyranosyl-D-mannose | - |
? | |
| maltotriose | - |
Dictyoglomus turgidum | 4-O-alpha-D-glucopyranosyl-4-O-alpha-D-glucopyranosyl-D-mannose | - |
? | |
| additional information | the enzyme catalyzes not only epimerization but also isomerization for beta-1,4- and alpha-1,4-gluco-oligosaccharides. No activity with trehalose, sophorose, laminaribiose, gentiobiose, or xylobiose | Dictyoglomus turgidum | ? | - |
? | |
| additional information | the enzyme catalyzes not only epimerization but also isomerization for beta-1,4- and alpha-1,4-gluco-oligosaccharides. No activity with trehalose, sophorose, laminaribiose, gentiobiose, or xylobiose | Dictyoglomus turgidum DSM 6724 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 47000, recombinant His-tagged enzyme, SDS-PAGE, 1 * 47200, about, recombinant His-tagged enzyme, sequence calculation | Dictyoglomus turgidum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| cellobiose 2-epimerase | - |
Dictyoglomus turgidum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
- |
Dictyoglomus turgidum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | 85 | purified recombinant His-tagged enzyme, thermal inactivation of the cellobiose 2-epimerase follows first-order kinetics and the half-lives of the enzyme at 65°C, 70°C, 75°C, 80°C, and 85°C are 79 h, 55 h, 35 h, 20 h, and 4.8 h, respectively | Dictyoglomus turgidum |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 89.4 | - |
lactose | purified recombinant His-tagged enzyme, pH 7.0, 70°C | Dictyoglomus turgidum | |
| 169.1 | - |
cellobiose | purified recombinant His-tagged enzyme, pH 7.0, 70°C | Dictyoglomus turgidum | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Dictyoglomus turgidum |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | structure, active site, and reaction mechanism of cellobiose 2-epimerase are similar to those of N-acyl-D-glucosamine 2-epimerase | Dictyoglomus turgidum |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.019 | - |
lactose | purified recombinant His-tagged enzyme, pH 7.0, 70°C | Dictyoglomus turgidum | |
| 0.037 | - |
cellobiose | purified recombinant His-tagged enzyme, pH 7.0, 70°C | Dictyoglomus turgidum | |
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