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Literature summary for 5.1.3.11 extracted from
- Lactulose production from lactose as a single substrate by a thermostable cellobiose 2-epimerase from Caldicellulosiruptor saccharolyticus (2012), Biores. Technol., 104, 668-672.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Caldicellulosiruptor saccharolyticus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | the enzyme is not inhibited by EDTA | Caldicellulosiruptor saccharolyticus |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | the enzyme is not activated by monovalent or divalent cations | Caldicellulosiruptor saccharolyticus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 47000 | - |
x * 47000, recombinant His-tagged enzyme, SDS-PAGE | Caldicellulosiruptor saccharolyticus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Caldicellulosiruptor saccharolyticus | A4XGA6 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme 10fold from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Caldicellulosiruptor saccharolyticus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 30 | - |
purified recombinnat His-tagged enzyme, substrate lactose, pH 7.5, 80°C | Caldicellulosiruptor saccharolyticus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| lactose | cellobiose 2-epimerase from Caldicellulosiruptor saccharolyticus can be used in the presence of borate for the production of lactulose from lactose, method development, overview | Caldicellulosiruptor saccharolyticus | lactulose | - |
? |  |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 47000, recombinant His-tagged enzyme, SDS-PAGE | Caldicellulosiruptor saccharolyticus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| cellobiose 2-epimerase | - |
Caldicellulosiruptor saccharolyticus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
- |
Caldicellulosiruptor saccharolyticus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
the enzyme displays first-order kinetics for thermal inactivation, and the half-lives (inactivation rate constants) at 65°C, 70°C, 75°C, and 80°C are 74 h, 34 h, 16h , and 5 h, respectively | Caldicellulosiruptor saccharolyticus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Caldicellulosiruptor saccharolyticus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| additional information | the activity of the cellobiose 2-epimerase is not affected by the addition of the cofactor ATP, FAD, FMN, NAD+, NADP+, NADH, or NADPH | Caldicellulosiruptor saccharolyticus |
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Release 2023.1 (January 2023)
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