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Literature summary for 5.1.3.11 extracted from

  • Taguchi, H.; Senoura, T.; Hamada, S.; Matsui, H.; Kobayashi, Y.; Watanabe, J.; Wasaki, J.; Ito, S.
    Cloning and sequencing of the gene for cellobiose 2-epimerase from a ruminal strain of Eubacterium cellulosolvens (2008), FEMS Microbiol. Lett., 287, 34-40.
    View publication on PubMed
Search for more literature for 5.1.3.11

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli [Eubacterium] cellulosolvens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
11.3
-
 cellobiose pH 7.5, 35°C [Eubacterium] cellulosolvens
72
-
 lactose pH 7.5, 35°C [Eubacterium] cellulosolvens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
46700
-
 x * 46963, calculated, x * 46700, SDS-PAGE [Eubacterium] cellulosolvens
46963
-
 x * 46963, calculated, x * 46700, SDS-PAGE [Eubacterium] cellulosolvens

Organism

Organism UniProt Comment Textmining
[Eubacterium] cellulosolvens B3XZI5
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme [Eubacterium] cellulosolvens

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cellobiose
-
 [Eubacterium] cellulosolvens 4-O-beta-D-glucopyranosyl-D-mannose
-
 ?
lactose
-
 [Eubacterium] cellulosolvens 4-O-beta-D-galactopyranosyl-D-mannose i.e. epilactose ?
additional information no substrates: N-acetyl-D-glucosamine, UDP-glucose, D-glucose 6-phosphate, glucose, mannose, galactose, fructose, xylose, arabinose, sophorose, laminaribiose, and gentiobiose [Eubacterium] cellulosolvens ?
-
 ?

Subunits

Subunits Comment Organism
? x * 46963, calculated, x * 46700, SDS-PAGE [Eubacterium] cellulosolvens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
 [Eubacterium] cellulosolvens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
28.5
-
 lactose pH 7.5, 35°C [Eubacterium] cellulosolvens
32.5
-
 cellobiose pH 7.5, 35°C [Eubacterium] cellulosolvens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8.5
-
 [Eubacterium] cellulosolvens