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Literature summary for 5.1.3.1 extracted from
- Deletion of D-ribulose-5-phosphate 3-epimerase (RPE1) induces simultaneous utilization of xylose and glucose in xylose-utilizing Saccharomyces cerevisiae (2015), Biotechnol. Lett., 37, 1031-1036 .
Application
| Application | Comment | Organism |
|---|---|---|
| biotechnology | efficient utilization of the available glucose and xylose in the lignocellulosic hydrolysates is the important issue for economic cellulosic ethanol production. Simultaneous utilization of xylose is realized by the coupling of glucose metabolism and xylose utilization through RPE1 deletion in xylose-utilizing Saccharomyces cerevisiae | Saccharomyces cerevisiae |
| synthesis | efficient utilization of the available glucose and xylose in the lignocellulosic hydrolysates is the important issue for economic cellulosic ethanol production. Simultaneous utilization of xylose is realized by the coupling of glucose metabolism and xylose utilization through RPE1 deletion in xylose-utilizing Saccharomyces cerevisiae | Saccharomyces cerevisiae |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene RPE1, subcloning in Escherichia coli DH5alpha, recombinant expression in Saccharomyces cerevisiae strain L2612 | Saccharomyces cerevisiae |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | deletion of D-ribulose-5-phosphate 3-epimerase (RPE1) inducing simultaneous utilization of xylose and glucose in xylose-utilizing Saccharomyces cerevisiae, construction of RPE1 deletion strain, SyBE_Sc17004 (RPE1::TRP1), by one-step gene disruption strategy. To create a xylose-utilizing yeast, XYL1 and XYL2 genes from Scheffersomyces stipitis and XKS1 from Saccharomyces cerevisiae strain L2612 are assembled and inserted into the genome of strain L2612 at the Delta-locus, resulting in strain SyBE_Sc17002. Strain SyBE_Sc17004 is created by deleting RPE1 in SyBE_Sc17002. Triggering xylose consumption by the deletion of RPE1 at the beginning of mixed sugar fermentation | Saccharomyces cerevisiae |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-Ribulose 5-phosphate | Saccharomyces cerevisiae | - |
D-Xylulose 5-phosphate | - |
r |  |
| D-Ribulose 5-phosphate | Saccharomyces cerevisiae ATCC 204508 / S288c | - |
D-Xylulose 5-phosphate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | P46969 | - |
- |
| Saccharomyces cerevisiae ATCC 204508 / S288c | P46969 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-Ribulose 5-phosphate | - |
Saccharomyces cerevisiae | D-Xylulose 5-phosphate | - |
r | |
| D-Ribulose 5-phosphate | - |
Saccharomyces cerevisiae ATCC 204508 / S288c | D-Xylulose 5-phosphate | - |
r | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| D-ribulose-5-phosphate 3-epimerase | - |
Saccharomyces cerevisiae |
| rpe1 | - |
Saccharomyces cerevisiae |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | deletion of D-ribulose-5-phosphate 3-epimerase (RPE1) induces simultaneous utilization of xylose and glucose in xylose-utilizing Saccharomyces cerevisiae | Saccharomyces cerevisiae |
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