Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, using 25% (w/v) PEG 3350, 100 mM Bis-Tris (pH 5.5), and 200 mM NaCl | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
D175A | inactive | Homo sapiens |
D37A | the mutation almost abolishes the enzymatic activity | Homo sapiens |
H35A | the mutation almost abolishes the enzymatic activity | Homo sapiens |
H70A | completely insoluble | Homo sapiens |
L12A | the mutation results in more than 50% decrease in the activity | Homo sapiens |
M141A | the mutation results in about 30% decrease in the activity | Homo sapiens |
M39A | the mutation results in about 10% decrease in the activity | Homo sapiens |
M72A | the mutation results in an almost 50% decrease in the activity | Homo sapiens |
S10A | the mutation almost abolishes the enzymatic activity | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | RPE uses Fe2+ for catalysis | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q96AT9 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-affinity column chromatography and Superdex G75 gel filtration | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-Ribulose 5-phosphate | - |
Homo sapiens | D-Xylulose 5-phosphate | - |
r |
Synonyms | Comment | Organism |
---|---|---|
D-ribulose 5-phosphate 3-epimerase | - |
Homo sapiens |
RPE | - |
Homo sapiens |