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Literature summary for 5.1.3.1 extracted from

  • Liang, W.; Ouyang, S.; Shaw, N.; Joachimiak, A.; Zhang, R.; Liu, Z.J.
    Conversion of D-ribulose 5-phosphate to D-xylulose 5-phosphate: new insights from structural and biochemical studies on human RPE (2011), FASEB J., 25, 497-504.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 25% (w/v) PEG 3350, 100 mM Bis-Tris (pH 5.5), and 200 mM NaCl Homo sapiens

Protein Variants

Protein Variants Comment Organism
D175A inactive Homo sapiens
D37A the mutation almost abolishes the enzymatic activity Homo sapiens
H35A the mutation almost abolishes the enzymatic activity Homo sapiens
H70A completely insoluble Homo sapiens
L12A the mutation results in more than 50% decrease in the activity Homo sapiens
M141A the mutation results in about 30% decrease in the activity Homo sapiens
M39A the mutation results in about 10% decrease in the activity Homo sapiens
M72A the mutation results in an almost 50% decrease in the activity Homo sapiens
S10A the mutation almost abolishes the enzymatic activity Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ RPE uses Fe2+ for catalysis Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q96AT9
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-affinity column chromatography and Superdex G75 gel filtration Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-Ribulose 5-phosphate
-
Homo sapiens D-Xylulose 5-phosphate
-
r

Synonyms

Synonyms Comment Organism
D-ribulose 5-phosphate 3-epimerase
-
Homo sapiens
RPE
-
Homo sapiens