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Literature summary for 5.1.3.1 extracted from

  • Chen, Y.R.; Larimer, F.W.; Serpersu, E.H.; Hartman, F.C.
    Identification of a catalytic aspartyl residue of D-ribulose 5-phosphate 3-epimerase by site-directed mutagenesis (1999), J. Biol. Chem., 274, 2132-2136.
    View publication on PubMed
Search for more literature for 5.1.3.1

Protein Variants

Protein Variants Comment Organism
D186A KM-value for D-ribulose 5-phosphate is increased 10fold, turnover-number is decreased to less than 0.1% of the wild-type value Spinacia oleracea
D186E KM-value for D-ribulose 5-phosphate is unaltered, turnover-number is decreased to less than 0.1% of the wild-type value Spinacia oleracea
D186N KM-value for D-ribulose 5-phosphate is unaltered, turnover-number is decreased to less than 0.1% of the wild-type value Spinacia oleracea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.22
-
 D-ribulose 5-phosphate wild-type enzyme Spinacia oleracea
0.28
-
 D-ribulose 5-phosphate mutant enzyme D186N Spinacia oleracea
0.3
-
 D-ribulose 5-phosphate mutant enzyme D186E Spinacia oleracea
2.1
-
 D-ribulose 5-phosphate mutant enzyme D186A Spinacia oleracea

Organism

Organism UniProt Comment Textmining
Spinacia oleracea Q43157
-
-

Purification (Commentary)

Purification (Comment) Organism
mutant enzymes D186A, D186N, D186E Spinacia oleracea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-Ribulose 5-phosphate
-
 Spinacia oleracea D-Xylulose 5-phosphate
-
 ?

Synonyms

Synonyms Comment Organism
Ru5P epimerase
-
 Spinacia oleracea

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00033
-
 D-ribulose 5-phosphate mutant enzyme D186A Spinacia oleracea
0.13
-
 D-ribulose 5-phosphate mutant enzyme D186N Spinacia oleracea
1.1
-
 D-ribulose 5-phosphate mutant enzyme D186E Spinacia oleracea
7100
-
 D-ribulose 5-phosphate wild-type enzyme Spinacia oleracea

pI Value

Organism Comment pI Value Maximum pI Value
Spinacia oleracea wild-type enzyme and mutant enzyme D186E, denaturing isoelectric focusing, pH gradient 5-8 6.8 6.3
Spinacia oleracea mutant enzymes D186A and D186N, denaturing isoelectric focusing, pH gradient 5-8
-
 6.6