Literature summary for 5.1.2.6 extracted from
Hoshiko, S.; Kunimoto, Y.; Arima, K.; Beppu, T.
Mechanism of L-alloisocitric acid fermentation: isocitrate epimerase activity in the cell-free extract of Penicillium purpurogenum (1982), Agric. Biol. Chem., 46, 143-151.
No PubMed abstract available
Activating Compound
Activating Compound |
Comment |
Organism |
Structure |
pyridoxal 5'-phosphate |
slight stimulation |
Talaromyces purpureogenus |
|
General Stability
General Stability |
Organism |
30% v/v glycerol plus 1 mM DTT stabilize against heat inactivation, slight protection by leupeptin, pepstatin, and PMSF |
Talaromyces purpureogenus |
Localization
Localization |
Comment |
Organism |
GeneOntology No. |
Textmining |
mitochondrion |
- |
Talaromyces purpureogenus |
5739 |
- |
Organism
Organism |
UniProt |
Comment |
Textmining |
Talaromyces purpureogenus |
- |
- |
- |
Purification (Commentary)
Purification (Comment) |
Organism |
partial |
Talaromyces purpureogenus |
Substrates and Products (Substrate)
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
(1R,2S)-1-Hydroxypropane-1,2,3-tricarboxylate |
i.e. threo-D-isocitrate |
Talaromyces purpureogenus |
(1S, 2S)-1-Hydroxypropane-1,2,3-tricarboxylate |
i.e. erythro-L-isocitrate |
? |
|
Temperature Stability [°C]
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Comment |
Organism |
20 |
- |
room temperature, half life: 12 h, with 30% glycerol, and 1 mM DTT, half-life: less than 3 h, without stabilizers |
Talaromyces purpureogenus |
pH Optimum
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
4.7 |
5.6 |
synthesis of erythro-isocitrate |
Talaromyces purpureogenus |
pH Range
pH Minimum |
pH Maximum |
Comment |
Organism |
3 |
8 |
3: about 35% of maximal activity, 8: about 30% of maximal activity, synthesis of erythro-isocitrate |
Talaromyces purpureogenus |