Crystallization (Comment) | Organism |
---|---|
of mutant K166R | Pseudomonas putida |
Protein Variants | Comment | Organism |
---|---|---|
K166E | K166R retains low level of racemase activity. K166R mutant catalyzes the elimination of Br- from only the (R)-enantiomer of (R,S)-p-(bromomethyl)mandelate | Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.34 | - |
(R)-mandelate | wild type | Pseudomonas putida | |
0.35 | - |
(S)-Mandelate | wild type | Pseudomonas putida | |
0.59 | - |
(S)-Mandelate | mutant K166R | Pseudomonas putida | |
1.4 | - |
(S)-Mandelate | mutant K166R | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Purification (Comment) | Organism |
---|---|
wild type and mutant K166R | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-mandelate | - |
Pseudomonas putida | L-mandelate | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.46 | - |
(S)-Mandelate | mutant K166R | Pseudomonas putida | |
480 | - |
(R)-mandelate | wild type enzyme | Pseudomonas putida |