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Literature summary for 5.1.1.8 extracted from
- Immuno-profiles of BALB/c mice inoculated with Salmonella vector delivering B-cell mitogen hydroxyproline epimerase (2018), Mol. Immunol., 95, 114-121 .
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | PrpA can be used as a protein adjuvant in a live Salmonella delivery system, in order to increase humoral responses effectively without major interference on the cell mediated immunity | Brucella abortus |
| molecular biology | PrpA can be used as a protein adjuvant in a live Salmonella delivery system, in order to increase humoral responses effectively without major interference on the cell mediated immunity | Brucella abortus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene prpA, live attenuated Salmonella typhimurium (ST) vector constitutively expressing heterologous Brucella abortus PrpA protein (ST-PrpA) is inoculated in BALB/c mice, recombinant expression of His-tagged Brucella abortus PrpA in Escherichia coli strain BL21 | Brucella abortus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| trans-4-hydroxy-L-proline | Brucella abortus | - |
cis-4-hydroxy-D-proline | - |
r |  |
| trans-4-hydroxy-L-proline | Brucella abortus 2308 | - |
cis-4-hydroxy-D-proline | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Brucella abortus | Q2YLF3 | - |
- |
| Brucella abortus 2308 | Q2YLF3 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinnat His-tagged Brucella abortus PrpA from Escherichia coli strain BL21 by nickel affinity chromatography | Brucella abortus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| trans-4-hydroxy-L-proline | - |
Brucella abortus | cis-4-hydroxy-D-proline | - |
r | |
| trans-4-hydroxy-L-proline | - |
Brucella abortus 2308 | cis-4-hydroxy-D-proline | - |
r | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 4-hydroxyproline 2-epimerase | - |
Brucella abortus |
| BaHyPRE | - |
Brucella abortus |
| Hydroxyproline epimerase | - |
Brucella abortus |
| PrpA | - |
Brucella abortus |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Brucella abortus | live attenuated Salmonella typhimurium (ST) vector constitutively expressing heterologous Brucella abortus S19 PrpA protein (ST-PrpA) is inoculated in BALB/c mice. No aggravation of morbidity is observed upon mice inoculation of ST-PrpA. Immunized mice show significantly quicker anti-Salmonella IgG responses as compared to ST only immunization, and an increase interleukin-4 production evident in in vitro pulsed mice splenocytes, splenocytes proliferative profiles and lymphocyte population evaluation, overview | up |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | 4-hydroxyproline 2-epimerase is a member of proline racemase family | Brucella abortus |
| physiological function | the enzyme 4-hydroxyproline 2-epimerase reversibly catalyse the interconversion of trans-4-hydroxy-L-proline and cis-4-hydroxy-D-proline. The Brucella proline racemase (PrpA) or hydroxyproline epimerase (BaHyPRE) also exhibit B-cell mitogenic properties thereby causing B-lymphocytes polyclonal activation and increase secretion of interleukin (IL)-10. Importance of PrpA in brucellosis | Brucella abortus |
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