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Literature summary for 5.1.1.8 extracted from

  • Watanabe, S.; Hiraoka, Y.; Endo, S.; Tanimoto, Y.; Tozawa, Y.; Watanabe, Y.
    An enzymatic method to estimate the content of L-hydroxyproline (2015), J. Biotechnol., 199, 9-16 .
    View publication on PubMed
Search for more literature for 5.1.1.8

Application

Application Comment Organism
analysis estimation of the content of L-hydroxyprolines using coupling systems with metabolic enzymes of the trans-4-hydroxy-L-proline pathway (hydroxyproline 2-epimerase (HypE) and cis-4-hydroxy-D-proline dehydrogenase (HypDH)) and the trans-3-hydroxy-L-proline pathway (trans-3-hydroxy-L-proline dehydratase (T3LHypD) and DELTA1-pyrroline-2-carboxylate reductase (Pyr2CR)) from microorganisms. A functional expression system of recombinant HypDH with a heterooligomeric structure is constructed in Escherichia coli cells. Enzymological characterization reveals that the beta-subunit acts as a catalytic subunit, and also that assembly with other subunit(s) improves the kinetics for cis-4-hydroxy-D-proline and thermostability. By using a spectrophotometric assay with different wavelengths, the contents of trans-4-hydroxy-L-proline and trans-3-hydroxy-L-proline are successfully estimated within the ranges of 0.004-1 mM and 0.05-1 mM, respectively, and are consistent with the contents determined by HPLC. This enzymatic method is used to measure the content of trans-4-hydroxy-L-proline in the acid-hydrolysate of collagen, and blood plasma Azospirillum brasilense

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
trans-4-hydroxy-L-proline Azospirillum brasilense no activity with L-proline cis-4-hydroxy-D-proline
-
 ?

Organism

Organism UniProt Comment Textmining
Azospirillum brasilense S6CGK1
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
trans-4-hydroxy-L-proline no activity with L-proline Azospirillum brasilense cis-4-hydroxy-D-proline
-
 ?

Synonyms

Synonyms Comment Organism
AbLhpA
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 Azospirillum brasilense
Hydroxyproline 2-epimerase
-
 Azospirillum brasilense
HypE
-
 Azospirillum brasilense

General Information

General Information Comment Organism
metabolism the enzyme is involved in the metabolic pathway of trans-4-hydroxy-L-proline Azospirillum brasilense